连作对大豆根际土壤细菌菌群结构的影响

doi:10.13560/j.cnki.biotech.bull.1985.2017-0728

摘要/Abstract

摘要： 土壤细菌的菌群结构是土壤生态环境质量的重要组成部分。为探明大豆连作种植后根际土壤细菌群落结构变化,试验选取连作0年大豆根际土样与连作2年大豆根际土壤,利用Illumina高通量测序技术对比研究了其中的细菌群落结构。结果表明,连作0年根际土壤中的细菌丰富度和多样性指数均高于连作2年大豆根际土壤中相应值。大豆根际土壤中的噬纤维菌科（Cytophagaceae）、鞘氨纯单胞菌（Sphingomonas）、慢生根瘤菌（Bradyrhizobium）及链霉菌属（Streptomyces）等一些有益菌的相对丰度要变化并不显著。大豆连作会降低土壤的细菌多样性,改变细菌菌群结构。但是在短期连作过程中,不同作物根际微生物类群的变化规律各不相同,难以用来推断作物连作所存在普遍现象。以期试验结果同时为缓解短期大豆连作障碍提高大豆产量提供一定的理论依据。

关键词: 连作, 大豆, 根际土壤, 细菌群落, Illumina高通量测序

Abstract: The flora structure of soil bacteria is an important part of soil ecological environment quality. In order to find out the changes of bacterial flora structure in the rhizosphere soil after continuous cropping of soybean,soybean rhizosphere soil samples of zero- and 2-year of continuous cropping were selected,and their bacterial flora structures were studied using Illumina high-throughput sequencing technique. The results showed that the bacterial abundances and diversity indexes in the rhizosphere soil of zero-year continuous cropping were higher than those of the rhizosphere soil of 2-year continuous cropping. The abundances of these beneficial bacteria Cytophagaceae,Sphingomonas,Bradyrhizobium,and Streptomyces varied insignificantly. Soybean continuous cropping reduced the bacterial diversity of the soil and changed the bacterial flora structure. However,during the short-term continuous cropping process,the variation pattern of rhizosphere microbes in different crops differed,thus it was difficult to infer a common phenomenon resulted from continuous cropping. The results are aimed to provide a theoretical basis for alleviating the short-term soybean continuous cropping obstacle and increasing the yield of soybean.

Key words: continuous cropping, soybean, rhizosphere soil, bacterial flora, Ilumina high-throughput sequencing

殷继忠, 李亮, 接伟光, 蔡柏岩. 连作对大豆根际土壤细菌菌群结构的影响[J]. 生物技术通报, 2018, 34(1): 230-238.

YIN Ji-zhong, LI Liang, JIE Wei-guang, CAI Bai-yan. Effects of Continuous Cropping on Bacterial Flora Structure in Soybean Rhizosphere Soil[J]. Biotechnology Bulletin, 2018, 34(1): 230-238.

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