生物技术通报 ›› 2019, Vol. 35 ›› Issue (4): 76-81.doi: 10.13560/j.cnki.biotech.bull.1985.2018-0907

• 研究报告 • 上一篇    下一篇

中华鳖MyoD1基因SNP鉴定及其与生长性状的关联分析

黄龙, 吴本丽, 何吉祥, 陈静, 宋光同, 汪翔, 张烨, 武松   

  1. 1. 安徽省农业科学院水产研究所,合肥 230031;
    2. 水产增养殖安徽省重点实验室,合肥 230031
  • 收稿日期:2018-10-22 出版日期:2019-04-26 发布日期:2019-05-05
  • 作者简介:黄龙,男,博士,助理研究员,研究方向:动物遗传育种与繁殖;E-mail:huanglong26@126.com
  • 基金资助:
    安徽省农业科学院院长青年创新基金(17B0509),安徽省科技攻关计划项目(1604a0702018),安徽省重点研究与开发计划项目(1704g07020118)

SNP Identification of MyoD1 Gene and Its Correlation with Growth Traits in Pelodiscus sinensis

HUANG Long, WU Ben-li, HE Ji-xiang, CHEN Jing, SONG Guang-tong, WANG Xiang, ZHANG Ye, WU Song   

  1. 1. Fisher Research Institute,Anhui Academy of Agricultural Sciences,Hefei 230031;
    2. Key Laboratory of Aquaculture & Stock Enhancement for Anhui Province,Hefei 230031;
  • Received:2018-10-22 Published:2019-04-26 Online:2019-05-05

摘要: 为了研究生肌决定因子1(MyoD1)基因多态性与中华鳖生长性状的相关性,采用直接测序法在MyoD1基因上共检测到6个SNP位点(T-49G、A-38G、C91T、A187T、C880T和T1522A),其中C880T位于外显子上,属于错义突变。对从同批繁殖、同块稻田养殖的2冬龄中华鳖群体中随机选取的178只个体中各位点的基因型进行检测。结果显示,所有位点在中华鳖群体中的平均有效等位基因数、平均观测杂合度和平均期望杂合度分别为1.636 5、0.349 3和0.375 4,除A187T 位点外,其余5个位点的基因型频率分布均符合Hardy-Weinberg定律。采用一般线性模型分析各位点与中华鳖生长性状之间的相关性,研究发现,T-49G位点GG基因型个体的背甲宽显著大于TT基因型,A-38G位点AG基因型个体的背甲宽显著大于AA基因型,A187T位点TT基因型个体的体高显著大于AA基因型,T1522A位点AA基因型个体的体质量显著大于TT、TA基因型,其余位点不同基因型个体间的生长性状均不存在显著差异。T-49G、A-38G、A187T和T1522A位点与生长性状显著相关,可作为中华鳖分子标记辅助育种的候选标记。

关键词: 中华鳖, 生肌决定因子1基因, 单核苷酸多态性标记, 生长性状, 关联分析

Abstract: In order to study the correlation between DNA polymorphisms of MyoD1 gene and growth traits of P. sinensis,direct sequencing method was used and 6 SNP loci(T-49G,A-38G,C91T,A187T,C880T and T1522A)in P. sinensis MyoD1 gene were identified. Among them,one SNP(C880T)was located in exons and missense mutation happened. Randomly selecting 178 randomly 2-winter-age P. sinensis bred in the same batch and cultivated in the same rice field,the genotypes of each SNP locus of them were detected. The results showed that the average effective number of allele(Ne),average observed heterozygosity(Ho),and average expected heterozygosity(He)of these SNPs was 1.636 5,0.349 3 and 0.375 4,respectively. Chi-square test showed that 5 SNP loci(T-49G,A-38G,C91T,C880T,and T1522A)were in Hardy-Weinberg equilibrium state in P. sinensis population. A general linear model was established for correlation analysis between different genotypes at SNP loci of MyoD1 gene and various growth traits. At the locus of T-49G,individuals with the GG genotype had significantly greater carapace widths than ones with the TT genotypes. At the locus of A-38G,individuals with the AG genotype had significantly greater carapace widths than ones with the AA genotypes. At the locus of A187T,individuals with the TT genotype had significantly greater heights than ones with the genotype AA. At the locus of T1522A,individuals with the AA genotype had significantly greater body weights than ones with the TT and TA genotypes. Other three SNP loci were not significantly correlated with growth traits. The growth-related locus T-49G,A-38G,A187T and T1522A could be useful candidate molecular markers in P. sinensis molecular marker-assisted selection.

Key words: Pelodiscus sinensis, myogenic differentiation 1 gene, single nucleotide polymorphism, growth traits, correlation analysis