小麦病程相关基因TaSec14的克隆及功能研究

doi:10.13560/j.cnki.biotech.bull.1985.2019-1043

摘要/Abstract

摘要： Sec14蛋白家族在植物中参与磷酸代谢、信号转导等基本生命过程。从感白粉病小麦品种京411中克隆到一个磷脂酰肌醇转运蛋白基因,其开放阅读框为1 212 bp,编码403个氨基酸,与二穗短柄草中磷脂酰肌醇转运蛋白PITP3（XP_010236107.1）有80.45%的同源性,含有SEC14保守结构域,命名为TaSec14。接种白粉菌后,TaSec14在京411及其近等基因系BJ-1中表达趋势一致,表达量均高于抗病小麦品种Brock。利用病毒介导的基因沉默技术敲减京411中的TaSec14基因后,BSMV∶TaSec14叶片上白粉菌孢子的成功侵染率低于GKP Buffer对照组,而畸形率高于对照组,表明降低TaSec14基因的表达能在一定程度上提高感病小麦京411对白粉菌的抗性。推测TaSec14基因可能在小麦与白粉菌互作过程具有调控作用。

关键词: 小麦, TaSec14基因, 白粉病, VIGS

Abstract: The Sec14 protein family in plants is involved in essential life processes such as phosphate metabolism and signal transduction. A phosphatidylinositol transporter gene was cloned from the powdery mildew-susceptible wheat cultivar Jing411. The length of open reading frame is 1 212 bp,encoding 403 amino acids. It contains a conserved SEC14 domain and has 80.45% homology with the phosphatidylinositol transporter PITP3（XP_010236107.1）in Brachypodium distachyon,designated as TaSec14. After inoculation with Blumeria graminis f. sp. tritici（Bgt）isolate,the expression of TaSec14 in Jing411 and its near isogenic line BJ-1 was consistent and higher than that in Brock. After knocking down the TaSec14 gene in Jing411 by virus-mediated gene silencing（VIGS）,the successful infection rate of the Bgt conidiaspores in the BSMV∶TaSec14 experimental group was lower than that in the GKP Buffer control group,and the deformity rate was higher than that of the control group. The above results indicated that reducing the expression of TaSec14 gene to some extent increased the resistance of susceptible wheat Jing411 to powdery mildew. It is speculated that TaSec14 gene may play a regulatory role in the interaction between wheat and powdery mildew.

Key words: wheat, TaSec14 gene, powdery mildew, VIGS

牛欢, 冯静云, 黄建国, 张超群, 张露露, 刘晓颖, 王振英. 小麦病程相关基因TaSec14的克隆及功能研究[J]. 生物技术通报, 2020, 36(6): 54-62.

NIU Huan, FENG Jing-yun, HUANG Jian-guo, ZHANG Chao-qun, ZHANG Lu-lu, LIU Xiao-ying, WANG Zhen-ying. Cloning and Functional Analysis of a Wheat Pathogenesis-Related Gene TaSec14[J]. Biotechnology Bulletin, 2020, 36(6): 54-62.

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