单细胞转录组测序技术在动物上的应用研究

doi:10.13560/j.cnki.biotech.bull.1985.2021-0523

摘要/Abstract

摘要：

细胞是机体最基本的结构组成及功能单位,细胞类型和功能由其整个转录表达谱决定,通过单细胞转录组测序可以获得单个细胞转录表达谱,由此以高精度分辨率鉴定细胞类型、细胞状态以及稀有类型细胞,从而可以在单细胞水平分析细胞动态变化及细胞间的关系,深入解析驱动细胞变化及细胞异常背后的分子细胞机制。随着单细胞测序技术稳定性和测序通量的提高,以及测序成本的降低,其在发育生物学、肿瘤、免疫及疾病等领域被广泛应用,研究对象主要涉及人及模式生物,在动物上的应用研究相对较少。本文主要介绍单细胞转录组测序技术及其生物学应用并综述目前其在动物上的一些开创性研究,以期为今后更好的在动物上应用单细胞转录组测序提供方法参考。

关键词: 单细胞转录组测序, 细胞类型, 生物学应用, 动物

Abstract:

Cell is the basic structural component and functional unit of organism. Cell type and function are determined by its whole transcriptional expression profile. Single-cell transcriptome sequencing can be used to obtain the transcriptional expression profile of a single cell,so as to identify cell types,cell states and rare types with high-precision resolution,thus to analyze the dynamic changes of cells and the relationship between cells at the single cell level,and further to decipher the molecular cellular mechanisms behind cell changes and cell abnormalities. With the stability and throughout improvement of single-cell sequencing,as well as the reduction of sequencing cost,single-cell transcriptome sequencing has been widely used in the fields of developmental biology,tumor,immunity and disease. However,these researches are mainly focused on human and model organisms,there are a few researches on animals. Therefore,the objective of this paper is to introduce the single-cell transcriptome sequencing and its biological application,and review some of its pioneering research in animals,aiming to provide a method reference for better application of single-cell transcriptome sequencing in animals.

Key words: single-cell transcriptome sequencing, cell type, biological application, animals

熊和丽, 沙茜, 刘韶娜, 相德才, 张斌, 赵智勇. 单细胞转录组测序技术在动物上的应用研究[J]. 生物技术通报, 2022, 38(3): 226-233.

XIONG He-li, SHA Qian, LIU Shao-na, XIANG De-cai, ZHANG Bin, ZHAO Zhi-yong. Application of Single-cell Transcriptome Sequencing in Animals[J]. Biotechnology Bulletin, 2022, 38(3): 226-233.

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