生物技术通报 ›› 2021, Vol. 37 ›› Issue (8): 12-24.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0815

• 代谢生物学专题 • 上一篇    下一篇

非常规酵母细胞工厂合成天然产物

叶敏(), 高教琪, 周雍进()   

  1. 1.中国科学院大连化学物理研究所生物技术研究部,大连 116023
    2.大连市能源生物技术重点实验室,大连 116023
  • 收稿日期:2021-06-29 出版日期:2021-08-26 发布日期:2021-09-10
  • 作者简介:叶敏,女,硕士研究生,研究方向:合成生物学、甲醇酵母代谢工程;E-mail: yemin18@dicp.ac.cn
  • 基金资助:
    国家重点研发计划(2018YFA0900300);国家自然科学基金优秀青年基金项目(21922812);大连化物所创新研究基金(DICPI202021)

Engineering Non-conventional Yeast Cell Factory for the Biosynthesis of Natural Products

YE Min(), GAO Jiao-qi, ZHOU Yong-jin()   

  1. 1. Division of Biotechnology,Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian 116023
    2. Dalian Key Laboratory of Energy Biotechnology,Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian 116023
  • Received:2021-06-29 Published:2021-08-26 Online:2021-09-10

摘要:

天然产物在医药、美容、食品等行业具有重要的应用,随着人们需求的增加,迫切需要绿色可持续的生产过程。近年来,微生物细胞工厂合成天然产物发展迅速,目前常用的细胞工厂宿主包括大肠杆菌和酿酒酵母。与此同时,非常规酵母因其性状独特,如高密度有氧发酵、耐受温度和pH范围广,底物谱广泛(长链糖、脂肪酸、甲醇等),逐渐成为极具潜力的细胞工厂宿主。本文综述了近年来非常规酵母在天然产物合成方面的研究进展,主要集中在萜类和黄酮类化合物的生物合成,并对常用的改造手段和策略进行了介绍。在此基础上,系统讨论了新型非常规酵母细胞工厂存在的优势和不足之处,并总结了非常规酵母细胞工厂合成天然产物的挑战和机遇。

关键词: 非常规酵母, 萜类化合物, 黄酮类化合物, 微生物细胞工厂, 合成生物学

Abstract:

Natural products have been widely applied in medical,cosmetic and food industries. With the increasing of people’s demand,the green sustainable producing process is urgently needed. Recently,microbial cell factories for synthesizing natural products have been rapidly developing,and the current common-used cell factory hosts include Saccharomyces cerevisiae and Escherichia coli. Non-conventional yeasts have becoming very potential cell factory hosts due to their specific advantages,such as high-cell-density aerobic fermentation,tolerance to a wide range of temperature and pH,and wide substrate spectrum(long-chain hydrocarbons,fatty acids and methanol). In this review,we summarized recent progresses on engineering non-conventional yeasts for the synthesis of natural products mainly terpenoids and flavonoids,and introduced common engineering methods and strategies. On this basis,we systematically discussed their strengths and weaknesses of novel non-conventional yeasts cell factories,and finally listed the challenges and opportunities while using non-conventional yeasts cell factories for the synthesis of natural products.

Key words: non-conventional yeasts, terpenoids, flavonoids, microbial cell factories, synthetic biology