m6A甲基化修饰相关酶基因在牛脂肪生成中的表达分析

doi:10.13560/j.cnki.biotech.bull.1985.2021-1231

摘要/Abstract

摘要：

探究参与m⁶A修饰的相关酶基因包括m⁶A甲基转移酶METTL3、METTL14、WTAP和m⁶A去甲基化酶FTO、ALKBH5,在牛不同组织以及前体脂肪细胞增殖和分化过程中的表达水平,为阐明m⁶A修饰在脂生成中的功能研究提供参考。使用实时荧光定量PCR技术检测基因在新生和成年秦川肉牛不同组织,以及在前体脂肪细胞中的表达;采用CCK-8和油红O染色检测前体脂肪细胞的生长曲线及其成脂分化中的脂滴形成情况。5个基因均在骨骼肌中低表达,其余组织中高表达,而在成年牛脂肪组织的表达水平均显著高于新生牛（P<0.05）。分离的前体脂肪细胞具有良好的生长状态且可正常成脂分化。在牛前体脂肪细胞增殖期,METTL3、METTL14和WTAP的表达在前期下降,之后有所上升但仍维持较低的表达水平。FTO的表达呈缓慢上升趋势。ALKBH5在增殖前期上升,中期下降,在第4天后不断增加（P<0.01）。在前体脂肪细胞分化过程中,METTL3、METTL14和FTO的表达模式基本一致,在分化前期下降,中期高表达,随后降低。而WTAP和ALKBH5的表达随分化进行逐渐增加。结果表明m⁶A修饰对秦川牛脂肪发育和沉积具有潜在的重要作用,且m⁶A甲基化酶可能在体外调控牛前体脂肪细胞的脂生成。

关键词: m⁶A, m⁶A甲基化酶, 牛, 前体脂肪细胞, 细胞增殖, 成脂分化

Abstract:

The aim of this study is to investigate the mRNA expressions of relevant genes,including METTL3,METTL14,WTAP for m⁶A methyltransferase,and FTO and ALKBH5 for m⁶A demethylaes,in bovine various tissues and in the proliferation and differentiation of preadipocytes,aiming to provide a reference for clarifying the role and mechanism of m⁶A modification in adipogenesis. Real-time quantitative PCR（RT-qPCR）was used to detect the mRNA expressions of m⁶A methylases in the various tissues of newborn and adult Qingchuan beef cattle. Then,CCK-8 treatment and oil-red O staining were adapted to detect the growth curves of the preadipocytes cells and lipid droplet formation during adipogenic differentiation. The 5 genes expressed lowly in skeletal muscles,and highly in rest tissues,while the mRNA expressions of them in the adipose tissue of adult cattle was significantly higher than that of newborn cattle（P < 0.05）. The isolated preadipocytes had a good growth state and adipogenic differentiation was accordance with the general pattern. The expressions of METTL3,METTL14 and WTAP decreased in the early stage of bovine preadipocytes proliferation,then increased and remained at low level. The expression of FTO showed a slow upward trend. The temporal expression of ALKBH5 increased in the early stage,decreased in the metaphase,and increased continuously after the day 4（P < 0.01）. The expression patterns of METTL3,METTL14 and FTO were similar in the adipogenic differentiation of bovine preadipocytes,which decreased in the early stage of differentiation,and highly expressed in the metaphase,then decreased. The expressions of WTAP and ALKBH5 increased with the progress of differentiation. Our results indicate that m⁶A modification plays a potentially important role in fat development and deposition of Qinchuan cattle,and m⁶A methylases may regulate the lipogenesis of bovine preadipocytes in vitro.

Key words: N⁶-methyladenosine（m⁶A）, m⁶A methylases, cattle, preadipocytes, cell proliferation, adipogenic differentiation

杨昕冉, 王建芳, 马鑫浩, 昝林森. m⁶A甲基化修饰相关酶基因在牛脂肪生成中的表达分析[J]. 生物技术通报, 2022, 38(7): 70-79.

YANG Xin-ran, WANG Jian-fang, MA Xin-hao, ZAN Lin-sen. Expression Analyses of m⁶A Methylase Genes in Bovine Adipogenesis[J]. Biotechnology Bulletin, 2022, 38(7): 70-79.

图/表 6

表1 RT-qPCR所用引物

Table 1 Primers for RT-qPCR

基因名称 Gene name	引物序列 Primer sequence（5'-3'）	产物长度 Product length/bp
METTL3	F：TCGAAAGCTGCACTTCAGAC	199
METTL3	R：TCCAACGCTCTGTGTAAGGG	199
METTL14	F：TGACATCAGAGAACTGACACCC	198
METTL14	R：AGGTCCAATCCTTCCCCAGA	198
WTAP	F：GCCTGGAAGTTTACGCCTGA	176
WTAP	R：TCCTGACTGCTTTTAAGCTCCT	176
FTO	F：AGCAGCGTACAACGTCACTT	193
FTO	R：AGGGTCGTCCTCACTTTCCT	193
ALKBH5	F：TACTTCTTCGGCGAGGGCTA	191
ALKBH5	R：TGGTAGTCGTTGATGACGGC	191
PPARγ	F：TGAAGAGCCTTCCAACTCCC	117
PPARγ	R：GTCCTCCGGAAGAAACCCTTG	117
C/EBPα	F：ATCTGCGAACACGAGACG	73
C/EBPα	R：CCAGGAACTCGTCGTTGAA	73
C/EBPβ	F：TTCCTCTCCGACCTCTTCTC	79
C/EBPβ	R：CCAGACTCACGTAGCCGTACT	79
FABP4	F：TGAGATTTCCTTCAAATTGGG	101
FABP4	R：CTTGTACCAGAGCACCTTCATC	101
GAPDH	F：AGTTCAACGGCACAGTCAAGG	124
GAPDH	R：ACCACATACTCAGCACCAGCA	124
β-actin	F：CATCGGCAATGAGCGGTTCC	147
β-actin	R：ACCGTGTTGGCGTAGAGGTC	147

图1 m6A甲基化相关酶基因在秦川肉牛不同组织中的表达 A-E：METTL3（A）、METTL14（B）、WTAP（C）、FTO（D）和ALKBH5（E）基因在新生秦川肉牛中的组织表达谱;F-J：METTL3（F）、METTL14（G）、WTAP（H）、FTO（I）和ALKBH5（J）基因在成年秦川肉牛中的组织表达谱;K：METTL3、METTL14、WTAP、FTO和ALKBH5在新生和成年秦川肉牛脂肪组织中的表达水平比较。A-J：*表示其他组织与心脏中表达水平的差异比较;#表示脂肪组织与三种骨骼肌中表达水平的差异比较。*/#表示P<0.05、差异显著,**/##表示P<0.01、差异极显著,无标记表示差异不显著（P>0.05）。K：*表示P<0.05、差异显著,**表示P<0.01、差异极显著

Fig. 1 Expressions of m6A methylation-related genes in different tissues of Qinchuan beef cattle A-E：Tissue mRNA expression profiles of METTL3（A）,METTL14（B）,WTAP（C）,FTO（D）and ALKBH5（E）in newborn Qinchuan beef cattle. F-J：Tissue mRNA expression profiles of METTL3（F）,METTL14（G）,WTAP（H）,FTO（I）and ALKBH5（J）in adult Qinchuan beef cattle. K：Comparison of expression levels of METTL3,METTL14,WTAP,FTO and ALKBH5 in the adipose tissues of newborn and adult Qinchuan beef cattle. A-J：*P < 0.05,** P < 0.01,other tissues versus heart;# P < 0.05,##P < 0.01,fat versus skeletal muscles;no superscripts indicate no significant difference（P > 0.05）. K：*P < 0.05,** P < 0.01

图2 秦川肉牛前体脂肪细胞的形态与生长曲线 A：秦川肉牛前体脂肪细胞增殖期不同生长时间点的表型观察（标尺：200 μm）;B：秦川肉牛前体脂肪细胞的生长曲线

Fig. 2 Morphology and growth curve of preadipocytes from Qinchuan beef cattle in vitroA：Phenotypic observation of preadipocytes proliferation in Qinchuan beef cattle at different growth points（scale bar：200 μm）. B：Growth curve of preadipocytes in Qinchuan beef cattle

图3 秦川肉牛前体脂肪细胞成脂分化检测 A：秦川肉牛前体脂肪细胞分化第3、6、9、12天的脂滴形成情况（标尺：20 μm）;B：脂肪生成关键因子PPARγ、C/EBPα、C/EBPβ和FABP4在秦川肉牛前体脂肪细胞成脂分化过程中的表达量检测。不同的大写字母表示差异极显著（P<0.05）,不同的小写字母表示差异显著（P<0.05）,相同字母表示差异不显著（P>0.05）。下同

Fig. 3 Detection of preadipocytes adipogenic differentiation in Qinchuan beef cattle A：Observation of lipid droplet formation on day 3,6,9 and 12 of culture in differentiation medium after the induction of adipogenic differentiation（scale bar：20 μm）;B：Relative mRNA expression of adipogenesis-related genes（PPARγ,C/EBPα,C/EBPβ and FABP4）during bovine preadipocytes differentiation. Different capital letters indicate very significant differences（P < 0.01）,different lowercase letters indicate significant differences（P < 0.05）,and the same letters indicate no significant differences（P > 0.05）. The same below

图4 m6A甲基化相关基因在前体脂肪细胞增殖过程中的时序表达谱

Fig. 4 Temporal expression profile of m6A methylation-related genes during preadipocytes proliferation

图5 m6A甲基化相关基因在前体脂肪细胞分化过程中的时序表达谱

Fig. 5 Temporal expression profile of m6A methylation-related genes during preadipocytes differentiation

参考文献 61

[1]	昝林森, 王洪程, 梅楚刚. 秦川牛肉用选育改良及产业化开发[J]. 农业生物技术学报, 2015, 23(1):135-140.
	Zan LS, Wang HC, Mei CG. Breeding and improvement of Qinchuan cattle and its beef industrialization[J]. J Agric Biotechnol, 2015, 23(1):135-140.
[2]	Rosen ED, MacDougald OA. Adipocyte differentiation from the inside out[J]. Nat Rev Mol Cell Biol, 2006, 7(12):885-896. doi: 10.1038/nrm2066 URL
[3]	Farmer SR. Transcriptional control of adipocyte formation[J]. Cell Metab, 2006, 4(4):263-273. doi: 10.1016/j.cmet.2006.07.001 URL
[4]	Zhu JG, Xia L, Ji CB, et al. Differential DNA methylation status between human preadipocytes and mature adipocytes[J]. Cell Biochem Biophys, 2012, 63(1):1-15. doi: 10.1007/s12013-012-9336-3 URL
[5]	Wang LF, Jin QH, Lee JE, et al. Histone H3K27 methyltransferase Ezh2 represses Wnt genes to facilitate adipogenesis[J]. PNAS, 2010, 107(16):7317-7322. doi: 10.1073/pnas.1000031107 URL
[6]	Liu J, Yue Y, Han D, et al. A METTL3-METTL14 complex mediates mammalian nuclear RNA N6-adenosine methylation[J]. Nat Chem Biol, 2014, 10(2):93-95. doi: 10.1038/nchembio.1432 URL
[7]	Ping XL, Sun BF, Wang L, et al. Mammalian WTAP is a regulatory subunit of the RNA N6-methyladenosine methyltransferase[J]. Cell Res, 2014, 24(2):177-189.
[8]	Jia G, Fu Y, Zhao X, et al. N6-methyladenosine in nuclear RNA is a major substrate of the obesity-associated FTO[J]. Nat Chem Biol, 2011, 7(12):885-887. doi: 10.1038/nchembio.687 URL
[9]	Zheng G, Dahl JA, Niu Y, et al. ALKBH₅ is a mammalian RNA demethylase that impacts RNA metabolism and mouse fertility[J]. Mol Cell, 2013, 49(1):18-29. doi: 10.1016/j.molcel.2012.10.015 URL
[10]	Wang X, Lu Z, Gomez A, et al. N⁶-methyladenosine-dependent regulation of messenger RNA stability[J]. Nature, 2014, 505(7481):117-120. doi: 10.1038/nature12730 URL
[11]	Fu Y, Dominissini D, Rechavi G, et al. Gene expression regulation mediated through reversible m⁶A RNA methylation[J]. Nat Rev Genet, 2014, 15(5):293-306. doi: 10.1038/nrg3724 URL
[12]	Wang X, Zhao BS, Roundtree IA, et al. N⁶-methyladenosine modulates messenger RNA translation efficiency[J]. Cell, 2015, 161(6):1388-1399. doi: 10.1016/j.cell.2015.05.014 URL
[13]	Shi H, Wei J, He C. Where, when, and how:context-dependent functions of RNA methylation writers, readers, and erasers[J]. Mol Cell, 2019, 74(4):640-650. doi: 10.1016/j.molcel.2019.04.025 URL
[14]	Frayling TM, Timpson NJ, et al. A common variant in the FTO gene is associated with body mass index and predisposes to childhood and adult obesity[J]. Science, 2007, 316(5826):889-894. doi: 10.1126/science.1141634 pmid: 17434869
[15]	Zhao X, Yang Y, Sun BF, et al. FTO-dependent demethylation of N⁶-methyladenosine regulates mRNA splicing and is required for adipogenesis[J]. Cell Res, 2014, 24(12):1403-1419. doi: 10.1038/cr.2014.151 pmid: 25412662
[16]	Wang X, Wu R, Liu Y, et al. m⁶A mRNA methylation controls autophagy and adipogenesis by targeting Atg5 and Atg7[J]. Autophagy, 2020, 16(7):1221-1235. doi: 10.1080/15548627.2019.1659617 URL
[17]	Merkestein M, Laber S, McMurray F, et al. FTO influences adipogenesis by regulating mitotic clonal expansion[J]. Nat Commun, 2015, 6:6792. doi: 10.1038/ncomms7792 pmid: 25881961
[18]	Kobayashi M, Ohsugi M, Sasako T, et al. The RNA methyltransferase complex of WTAP, METTL3, and METTL14 regulates mitotic clonal expansion in adipogenesis[J]. Mol Cell Biol, 2018, 38(16):e00116-18. DOI: 10.1128/mcb.00116-18. doi: 10.1128/mcb.00116-18
[19]	Xie W, Ma LL, Xu YQ, et al. METTL3 inhibits hepatic insulin sensitivity via N6-methyladenosine modification of Fasn mRNA and promoting fatty acid metabolism[J]. Biochem Biophys Res Commun, 2019, 518(1):120-126. doi: 10.1016/j.bbrc.2019.08.018 URL
[20]	Yao Y, Bi Z, et al. METTL3 inhibits BMSC adipogenic differentiation by targeting the JAK1/STAT5/C/EBPβ pathway via an m⁶A-YTHDF2-dependent manner[J]. FASEB J, 2019(6):7529-7544.
[21]	Wang Y, Gao M, Zhu F, et al. METTL3 is essential for postnatal development of brown adipose tissue and energy expenditure in mice[J]. Nat Commun, 2020, 11(1):1648. doi: 10.1038/s41467-020-15488-2 URL
[22]	Tao XL, Chen JN, Jiang YZ, et al. Transcriptome-wide N ^ 6-methyladenosine methylome profiling of porcine muscle and adipose tissues reveals a potential mechanism for transcriptional regulation and differential methylation pattern[J]. BMC Genom, 2017, 18(1):1-13. doi: 10.1186/s12864-016-3406-7 URL
[23]	Cheng BH, Leng L, et al. Profiling of RNA N⁶-methyladenosine methylation reveals the critical role of m⁶A in chicken adipose deposition[J]. Front Cell Dev Biol, 2021, 9:590468. doi: 10.3389/fcell.2021.590468 URL
[24]	Wei CM, Gershowitz A, Moss B. Methylated nucleotides block 5' Terminus of HeLa cell messenger RNA[J]. Cell, 1975, 4(4):379-386. pmid: 164293
[25]	Dominissini D, Moshitch-Moshkovitz S, Schwartz S, et al. Topology of the human and mouse m6A RNA methylomes revealed by m⁶A-seq[J]. Nature, 2012, 485(7397):201-206. doi: 10.1038/nature11112 URL
[26]	Meyer KD, Saletore Y, Zumbo P, et al. Comprehensive analysis of mRNA methylation reveals enrichment in 3' UTRs and near stop codons[J]. Cell, 2012, 149(7):1635-1646. doi: 10.1016/j.cell.2012.05.003 pmid: 22608085
[27]	Lin S, Choe J, Du P, et al. The m(6)A methyltransferase METTL3 promotes translation in human cancer cells[J]. Mol Cell, 2016, 62(3):335-345. doi: 10.1016/j.molcel.2016.03.021 URL
[28]	Yang D, Qiao J, Wang G, et al. N⁶-Methyladenosine modification of lincRNA 1281 is critically required for mESC differentiation potential[J]. Nucleic Acids Res, 2018, 46(8):3906-3920. doi: 10.1093/nar/gky130 URL
[29]	Bertero A, Brown S, Madrigal P, et al. The SMAD2/3 interactome reveals that TGFβ controls m⁶A mRNA methylation in pluripotency[J]. Nature, 2018, 555(7695):256-259. doi: 10.1038/nature25784 URL
[30]	Batista PJ, Molinie B, Wang J, et al. M(6)A RNA modification controls cell fate transition in mammalian embryonic stem cells[J]. Cell Stem Cell, 2014, 15(6):707-719. doi: 10.1016/j.stem.2014.09.019 pmid: 25456834
[31]	Zhang XX, Yao YL, Han JH, et al. Longitudinal epitranscriptome profiling reveals the crucial role of N⁶-methyladenosine methylation in porcine prenatal skeletal muscle development[J]. J Genet Genom, 2020, 47(8):466-476. doi: 10.1016/j.jgg.2020.07.003 URL
[32]	Wang X, Huang N, et al. FTO is required for myogenesis by positively regulating mTOR-PGC-1α pathway-mediated mitochondria biogenesis[J]. Cell Death Dis, 2017, 8(3):e2702.
[33]	Song T, Yang Y, Wei H, et al. Zfp217 mediates m⁶A mRNA methylation to orchestrate transcriptional and post-transcriptional regulation to promote adipogenic differentiation[J]. Nucleic Acids Res, 2019, 47(12):6130-6144. doi: 10.1093/nar/gkz312 URL
[34]	Xu T, Xu Z,Lu L,et al. Transcriptome-wide study revealed m⁶A reg-ulation of embryonic muscle development in Dingan goose(Anser cygnoides orientalis)[J]. BMC Genomics, 2021, 22(1):270. doi: 10.1186/s12864-021-07556-8 URL
[35]	朱琳娜. FTO、METTL3基因表达对猪脂肪细胞mRNA N⁶-甲基腺苷水平及脂肪沉积的影响研究[D]. 杭州: 浙江大学, 2014.
	Zhu LN. Effect of FTO, METTL3 Gene expression on mRNA m⁶A methylation and lipid metabolism in porcine subcutaneous adipocytes[D]. Hangzhou: Zhejiang University, 2014.
[36]	Fredriksson R, Hägglund M, Olszewski PK, et al. The obesity gene, FTO, is of ancient origin, up-regulated during food deprivation and expressed in neurons of feeding-related nuclei of the brain[J]. Endocrinology, 2008, 149(5):2062-2071. doi: 10.1210/en.2007-1457 pmid: 18218688
[37]	马子玉. METTL3对猪干细胞多能性的调控作用[D]. 杨凌: 西北农林科技大学, 2018.
	Ma ZY. METTL3 regulates the pluripotency of porcine pluripotent stem cells[D]. Yangling: Northwest A & F University, 2018.
[38]	Bravard A, Lefai E, et al. FTO is increased in muscle during type 2 diabetes, and its overexpression in myotubes alters insulin signaling, enhances lipogenesis and ROS production, and induces mitochondrial dysfunction[J]. Diabetes, 2011, 60(1):258-268. doi: 10.2337/db10-0281 pmid: 20943749
[39]	Chen JQ, Zhou XH, et al. FTO-dependent function of N6-methyladenosine is involved in the hepatoprotective effects of betaine on adolescent mice[J]. J Physiol Biochem, 2015, 71(3):405-413. doi: 10.1007/s13105-015-0420-1 URL
[40]	Aggarwal S, Pittenger MF. Human mesenchymal stem cells modulate allogeneic immune cell responses[J]. Blood, 2005, 105(4):1815-1822. doi: 10.1182/blood-2004-04-1559 URL
[41]	Rodriguez AM, Elabd C, Delteil F, et al. Adipocyte differentiation of multipotent cells established from human adipose tissue[J]. Biochem Biophys Res Commun, 2004, 315(2):255-263. doi: 10.1016/j.bbrc.2004.01.053 URL
[42]	Xia T, Wu X, Cao M, et al. The RNA m⁶A methyltransferase METTL3 promotes pancreatic cancer cell proliferation and invasion[J]. Pathol Res Pract, 2019, 215(11):152666. doi: 10.1016/j.prp.2019.152666 URL
[43]	Han J, Wang JZ, et al. METTL3 promote tumor proliferation of bladder cancer by accelerating pri-miR221/222 maturation in m⁶A-dependent manner[J]. Mol Cancer, 2019, 18(1):110. doi: 10.1186/s12943-018-0931-9 URL
[44]	Liu J, et al. m⁶A mRNA methylation regulates AKT activity to promote the proliferation and tumorigenicity of endometrial cancer[J]. Nat Cell Biol, 2018, 20(9):1074-1083. doi: 10.1038/s41556-018-0174-4 URL
[45]	徐晓东. N⁶-甲基嘌呤(m⁶A)甲基转移酶METTL14对胰腺癌增殖和侵袭转移的影响及其机制研究[D]. 武汉: 华中科技大学, 2017.
	Xu XD. The effect METTL14 on prolifetation and metastasis of pancreatic cancer cells and its mechanisms[D]. Wuhan: Huazhong University of Science and Technology, 2017.
[46]	Small TW, Bolender Z, Bueno C, et al. Wilms’ tumor 1-associating protein regulates the proliferation of vascular smooth muscle cells[J]. Circ Res, 2006, 99(12):1338-1346. doi: 10.1161/01.RES.0000252289.79841.d3 URL
[47]	Horiuchi K, Umetani M, Minami T, et al. Wilms’ tumor 1-associating protein regulates G2/M transition through stabilization of cyclin A2 mRNA[J]. PNAS, 2006, 103(46):17278-17283. doi: 10.1073/pnas.0608357103 pmid: 17088532
[48]	Kong Y, Wu R, et al. Wilms’ tumor 1-associating protein contributes to psoriasis by promoting keratinocytes proliferation via regulating cyclinA2 and CDK2[J]. Int Immun, 2020, 88:106918.
[49]	Su R, Dong L, Li C, et al. R-2HG exhibits anti-tumor activity by targeting FTO/m⁶a/MYC/CEBPA signaling[J]. Cell, 2018, 172(1/2):90-105. e23. doi: 10.1016/j.cell.2017.11.031 URL
[50]	Zhang S, Zhao BS, Zhou A, et al. m⁶A demethylase ALKBH₅ maintains tumorigenicity of glioblastoma stem-like cells by sustaining FOXM1 expression and cell proliferation program[J]. Cancer Cell, 2017, 31(4):591-606. e6. doi: 10.1016/j.ccell.2017.02.013 URL
[51]	方婷晓. m⁶A去甲基化酶ALKBH5抑制食管鳞癌的增殖、侵袭和迁移[D]. 广州: 南方医科大学, 2019.
	Fang TX. M⁶A demethylase ALKBH₅ inhibits proliferation, migration and invasion of esophageal squamous cell carcinoma[D]. Guangzhou: Southern Medical University, 2019.
[52]	Wu R, Liu Y, Yao Y, et al. FTO regulates adipogenesis by controlling cell cycle progression via m⁶A-YTHDF₂ dependent mechanism[J]. Biochim Biophys Acta Mol Cell Biol Lipids, 2018, 1863(10):1323-1330.
[53]	任倩. FTO通过抑制线粒体UPR缓解小鼠脂肪细胞凋亡的作用机制[D]. 杨凌: 西北农林科技大学, 2019.
	Ren Q. The mechanism of FTO on suppressing mice adipocytes apoptosis by reducing mitochondrial UPR[D]. Yangling: Northwest A & F University, 2019.
[54]	Kang H, Zhang Z, Yu L, et al. FTO reduces mitochondria and promotes hepatic fat accumulation through RNA demethylation[J]. J Cell Biochem, 2018, 119(7):5676-5685. doi: 10.1002/jcb.26746 URL
[55]	Jia X, Nie Q, Lamont SJ, et al. Variation in sequence and expression of the avian FTO, and association with glucose metabolism, body weight, fatness and body composition in chickens[J]. Int J Obes:Lond, 2012, 36(8):1054-1061. doi: 10.1038/ijo.2011.221 URL
[56]	Cai M, Liu Q, Jiang Q, et al. Loss of m⁶ A on FAM134B promotes adipogenesis in porcine adipocytes through m⁶ A-YTHDF2-dependent way[J]. IUBMB Life, 2019, 71(5):580-586. doi: 10.1002/iub.1974 URL
[57]	Jiang Q, Sun B, Liu Q, et al. MTCH₂ promotes adipogenesis in intramuscular preadipocytes via an m⁶A-YTHDF1-dependent mechanism[J]. FASEB J, 2019, 33(2):2971-2981. doi: 10.1096/fj.201801393RRR pmid: 30339471
[58]	李冬. METTL3对奶牛乳腺上皮细胞乳蛋白和乳脂肪合成的调控及其作用机理[D]. 哈尔滨: 东北农业大学, 2017.
	Li D. The regulatory mechanism of METTL3 on milk protein and milk fat synthesis in bovine mammary epithelial cells[D]. Harbin: Northeast Agricultural University, 2017.
[59]	Zhong X, Yu J, Frazier K, et al. Circadian clock regulation of hepatic lipid metabolism by modulation of m⁶A mRNA methylation[J]. Cell Rep, 2018, 25(7):1816-1828. e4. doi: 10.1016/j.celrep.2018.10.068 URL
[60]	Tews D, Fischer-Posovszky P, Wabitsch M. Regulation of FTO and FTM expression during human preadipocyte differentiation[J]. Horm Metab Res, 2011, 43(1):17-21. doi: 10.1055/s-0030-1265130 pmid: 20865646
[61]	Friebe D, Löffler D, Schönberg M, et al. Impact of metabolic regulators on the expression of the obesity associated genes FTO and NAMPT in human preadipocytes and adipocytes[J]. PLoS One, 2011, 6(6):e19526.

m⁶A甲基化修饰相关酶基因在牛脂肪生成中的表达分析

Expression Analyses of m⁶A Methylase Genes in Bovine Adipogenesis

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 6

参考文献 61

相关文章 15

编辑推荐

Metrics

本文评价

[1]	曾虹, 曾睿琳, 付伟, 吉文汇, 兰道亮. 牛诱导多能干细胞的建立及应用研究进展[J]. 生物技术通报, 2023, 39(5): 130-141.
[2]	郑焕, 林冬梅, 刘峻源, 张引莲, 林标声, 林占熺, 李晶. 基于LC-QTOF-MS代谢组学解析牛樟芝子实体和菌丝体中氨基酸代谢差异[J]. 生物技术通报, 2023, 39(5): 254-266.
[3]	陈晓萌, 张雪静, 张欢, 张宝江, 苏艳. 重组牛乳源金黄色葡萄球菌GapC蛋白优势B细胞抗原表位的预测和筛选[J]. 生物技术通报, 2023, 39(5): 306-313.
[4]	刘雄伟, 刘畅, 曾宪法, 杨小英, 俸婷婷, 赵杰宏, 周英. 朱砂根叶绿体全基因组解析及系统发育分析[J]. 生物技术通报, 2023, 39(1): 232-242.
[5]	张岩峰, 丁燕玲, 马应, 周小南, 杨朝云, 史远刚, 康晓龙. 肉牛剩余采食量相关瘤胃及粪便微生物特征比较分析[J]. 生物技术通报, 2023, 39(1): 295-304.
[6]	王楠, 张瑞, 潘阳阳, 何翃宏, 王靖雷, 崔燕, 余四九. 牦牛TGF-β1基因克隆及在雌性生殖系统主要器官中的表达定位[J]. 生物技术通报, 2022, 38(6): 279-290.
[7]	王万顺, 付强, 魏玉荣, 胡新艳, 陈俊贞, 李泽宇, 史慧君. 慢病毒介导Occludin过表达影响BVDV感染BALB/c小鼠的研究[J]. 生物技术通报, 2022, 38(6): 291-298.
[8]	李宇航, 王兴平, 杨箭, 罗仍卓么, 任倩倩, 魏大为, 马云. miR-665在奶牛乳腺上皮细胞炎症中的表达及功能分析[J]. 生物技术通报, 2022, 38(5): 159-168.
[9]	蒋旭东, 刘宇, 邬建飞, 胡双阁, 卢建远, 字向东. 牦牛FGG组织表达与雌性生殖器官中定位分析[J]. 生物技术通报, 2022, 38(11): 286-294.
[10]	郑青波, 叶娜, 张哓兰, 包鹏甲, 王福彬, 任稳稳, 廖月姣, 阎萍, 潘和平. 天祝白牦牛退行期毛囊细胞亚群鉴定以及特征基因生物信息学分析[J]. 生物技术通报, 2022, 38(10): 262-272.
[11]	付强, 陈俊贞, 郭妍婷, 姚刚, 冉多良, 史慧君. 应用CRISPR/Cas9技术敲除SERPINF2基因对牛病毒性腹泻病毒复制的影响[J]. 生物技术通报, 2021, 37(5): 267-272.
[12]	赵旭, 王文丽, 李娟. 腐殖酸煤对牛粪好氧堆肥臭气释放量及微生物群落多样性的影响[J]. 生物技术通报, 2021, 37(12): 104-112.
[13]	王晋鹏, 罗仍卓么, 王兴平, 杨箭, 贾立, 马云, 魏大为. 奶牛乳腺炎治疗及抗炎分子机制的研究进展[J]. 生物技术通报, 2021, 37(12): 212-219.
[14]	李晶, 冯娜, 王升陽, 林占熺. 牛樟芝化学成分及其药理作用研究进展[J]. 生物技术通报, 2021, 37(11): 14-31.
[15]	付强, 郭妍婷, 陈俊贞, 王金泉, 史慧君. 牛病毒性腹泻病毒离子孔道蛋白p7多肽多克隆抗体的制备和鉴定[J]. 生物技术通报, 2021, 37(10): 137-142.