Screening of Pseudomonas Strains Producing High-yield Siderophore and Its Utilization of Iron Hydroxides

doi:10.13560/j.cnki.biotech.bull.1985.2015.09.025

Abstract

Abstract: The aim of this study is to isolate the microorganisms producing high-yield siderophore, investigate antimicrobial activity of siderophore against pathogenic bacteria, and study the effect of siderophore on the utilization of insoluble poorly crystalline iron hydroxides(PICH). CAS method was used for isolating microorganisms producing high-yield siderophore. Agar diffusion method and growth inhibition assay were used to test antimicrobial activities of siderophore. Sequence alignment of 16S rRNA gene was used for strain identification. Bacterial growth was measured to investigate the utilization of insoluble PCIH by siderophore. Totally, 172 siderophore-producing strains were isolated from soil samples, including 13 potentially strong siderophore producers(PSSP). The analysis of antimicrobial activities of siderophore showed that only the supernatant of Z158 culture had a pronounced inhibiting effect on the growth of Staphylococcus aureus, Micrococcus luteus, Proteus vulgaris, and Vibrio parahemolyticus, and the inhibition efficiency was 51.3%, 50.2%, 37.1%, and 28.0%, respectively. Homology analysis of 16S rRNA gene sequence identified strain Z158 as Pseudomonas aeruginosa. Moreover, with insoluble PICH as sole iron source, the biomass of Z158 increased by 46.1% at 24 h after incubation. In conclusion, the siderophore secreted by P. aeruginosa Z158 could inhibit the growth of pathogenic bacteria and acquire iron element from insoluble PCIH.

Key words: siderophore, Pseudomonas aeruginosa, antimicrobial activity, poorly crystalline iron hydroxides

Zhu Huiming, Zhang Yan, Yang Hongjiang. Screening of Pseudomonas Strains Producing High-yield Siderophore and Its Utilization of Iron Hydroxides[J]. Biotechnology Bulletin, 2015, 31(9): 177-182.

References

[1]Hider RC, Kong X. Chemistry and biology of siderophores[J]. Natural Product Reprots, 2010, 27(5)：637-657.
[2]Heraman L, Maurice P, Sposito G. Iron acquisition from hydrous Fe(III)-oxides by an aerobic Pseudomonas sp. [J]. Chemical Geology, 1996, 132：25-31.
[3]Dehner CA, Awaya JD, Maurice PA, et al. Roles of siderophores, oxalate and ascorbate in mobilization of iron from hematite by the aerobic bacterium Pseudomonas mendocina[J]. Apply Environment Microbiology, 2010, 76(7)：2041-2048.
[4]朱亚玲, 毛得奖, 韩宁. 假单胞菌铁载体及色素研究[J]. 微生物学通报, 2013, 40(3)：500-516.
[5]Rachid D, Ahmed B. Effect of iron and growth inhibitors on siderophores production by Pseudomonas fluorescens[J]. African Journal of Biotechnology, 2005, 4(7)：697-702.
[6]Lin Y, Du DL, Si CC, et al. Potential biocontrol Bacillus sp. strains isolated by an improved method from vinegar waste compost exhibit antibiosis against fungal pathogens and promote growth of cucumbers[J]. Biological Control, 2014, 71：7-15.
[7]Pérez MS, Cabirol N, George TR, et al. O-CAS, a fast and universal method for siderophore detection[J]. Journal of Microbiology Methods, 2007, 70(1)：127-131.
[8]Takahiro Y, Kenichiro H, Hiroshi O. Dissolution of iron hydroxides by marine bacterial siderophore[J]. Chemical Geology, 2002, 184：1-9.
[9]Lee J, Postmaster A, Soon HP, et al. Siderophore production by actinomycetes isolates from two soil sites in Western Australia[J]. Biometals, 2012, 25(2)：285-296.
[10]谢志雄, 赵翔, 陈绍兴, 等. 适合高产铁载体细菌筛选、检测体系的改进与探析[J]. 微生物学通报, 2006, 33(6)：95-98.
[11]陈绍兴, 赵翔, 谢志雄等. 高产铁载体荧光假单胞菌Pseudomonas fluorescens sp-f的筛选鉴定及其铁载体特性研究[J]. 微生物学报, 2006, 46(5)：691-695.
[12]Wafaa MH, Mostafa AE. Production and optimization of Pseudomonas fluorescens biomass and metabolites for biocontrol of strawberry grey mould[J]. American Journal of Plant Sciences, 2012, 3(7)：836-845.
[13]Sambrook J, Russell DW. Molecular Cloning：A Laboratory manual[M] 3rd ed. New York：Cold Spring Harbor Press, 2001.
[14]Meyer JM, Abdallah MA. The Fluorescent Pigment of Pseudomonas fluorescens：biosynthesis, purification and physicochemical properties[J]. Journal of General Microbiology, 1978, 107：319-328.
[15]Mattila T, Raaska L. Effects of iron level on the antagonistic action of siderophores from non-pathogenic Staphylococcus spp.[J]. Journal of Industrial Microbiology, 1995, 15：480-485.
[16]Balabaskar P, Meera T. Isolation and characterization of Pseudomonas Fluorescens from Rice Fileds[J]. International Journal of Food, 2012, 2(1)：113-120.
[17]Beneduzi A, Ambrosini A, Passaglia LM. Plant growth-promoting rhizobacteria(PGPR)：Their potential as antagonists and biocontrol agents[J]．Genetics and Molecular Biology, 2012, 35(4)：1044-1051．
[18]Stephan MK. Iron oxide dissolution and solubility in the presence of siderophores[J]. Aquatic Sciences-Research Across Boundaries, 2004, 66(1)：3-18.
[19]Patricia AM, David AA, Larry EH, et al. Siderophore production by an aerobic Pseudomonas mendocina bacterium in the presence of kaolinite[J]. Chemical Geology, 2002, 188：161-170.