Correlation Between FSHR and ESR1 and Breeding Traits in Leizhou Black Duck

doi:10.13560/j.cnki.biotech.bull.1985.2019-0094

Abstract

Abstract: The aim of this study is to explore effect of the laying-related gene FSHR and ESR1 on Leizhou black duck. Quantitative PCR was employed to investigate the expression pattern of FSHR and ESR1 in hypothalamus-hypophysis-ovary axis of 0-120 d Leizhou black duck,and PCR-SSCP was used for analyzing the correlation between FSHR and ESR1 with breeding traits. The results showed that the overall expression trend of FSHR increased stably at 0-90 d and decreased slightly at 90-120 d in hypothalamus and ovary,but there was no significant difference（P>0.05）. The overall expression trend of ESR1 increased in 0-120 d but there was fluctuation at 90 d in ovary and difference was not significant（P>0.05）. The SNP for FSHR（g.856937G>A,g.922947A>G）and ESR1（g.190744A>G）were significantly associated with laying performance（P<0.05）,but were not correlated with egg quality（P>0.05）. The results suggest FSHR and ESR1 play an important role in early gonad development in Leizhou black duck,and three identified SNPs may be used as genotype maker for egg laying.

Key words: Leizhou black duck, FSHR, ESR1, SNP, breeding traits

ZOU Kun, CUI Hong-yan, XUE Yuan, ZHANG Shao-wei, LU Li-li, ZHAO Zhi-hui, SU Ying. Correlation Between FSHR and ESR1 and Breeding Traits in Leizhou Black Duck[J]. Biotechnology Bulletin, 2019, 35(8): 118-126.

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