Cloning, Expressing and Functional Analysis of Na+/H+ Antiporter Gene OpNHX1 from Olimarabidopsis pumila in Xinjiang

Abstract

Abstract: A NHX1 gene, designated as OpNHX1（GenBank Accession No. KC200248）was isolated from Olimarabidopsis pumila, a plant for stress tolerance research. The cDNA of OpNHX1 was 2 153 bp in full length, contained an open reading frame（ORF）of 1 605 bp encoding a peptide of 534 amino acids residues. Phylogenetic analysis revealed that OpNHX1 had high sequence similarities with AtNHX1 and EhNHX1 and they belong to the same clade. The expression of OpNHX1 is induced by salt, PEG, cold, and abscisic acid. OpNHX1 is detected in roots, stems, leaves, flowers and siliques of Olimarabidopsis pumila, with the highest expression in stems. Importantly, overexpression of OpNHX1 in Arabidopsis thaliana improved the survival rate of transgenic plants under salt stress. This study provides valuable information to understand more about the molecular mechanism of salt tolerance in plant. Heterologous expression of OpNHX1 in W303-1B Δnhx1 yeast Na⁺/H⁺ antiporter mutants showed functional complementation.

Key words: Na⁺/H⁺ antiporter, Olimarabidopsis pumila, Stress tolerance, Functional complementation in yeast

Zhao Yunxia, Guo Danli, Wei Yanling, Huang Xianzhong. Cloning, Expressing and Functional Analysis of Na⁺/H⁺ Antiporter Gene OpNHX1 from Olimarabidopsis pumila in Xinjiang[J]. Biotechnology Bulletin, 2014, 0(7): 74-80.

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Cloning, Expressing and Functional Analysis of Na⁺/H⁺ Antiporter Gene OpNHX1 from Olimarabidopsis pumila in Xinjiang

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