%0 Journal Article %A ZOU Kun %A CUI Hong-yan %A XUE Yuan %A ZHANG Shao-wei %A LU Li-li %A ZHAO Zhi-hui %A SU Ying %T Correlation Between FSHR and ESR1 and Breeding Traits in Leizhou Black Duck %D 2019 %R 10.13560/j.cnki.biotech.bull.1985.2019-0094 %J Biotechnology Bulletin %P 118-126 %V 35 %N 8 %X The aim of this study is to explore effect of the laying-related gene FSHR and ESR1 on Leizhou black duck. Quantitative PCR was employed to investigate the expression pattern of FSHR and ESR1 in hypothalamus-hypophysis-ovary axis of 0-120 d Leizhou black duck,and PCR-SSCP was used for analyzing the correlation between FSHR and ESR1 with breeding traits. The results showed that the overall expression trend of FSHR increased stably at 0-90 d and decreased slightly at 90-120 d in hypothalamus and ovary,but there was no significant difference(P>0.05). The overall expression trend of ESR1 increased in 0-120 d but there was fluctuation at 90 d in ovary and difference was not significant(P>0.05). The SNP for FSHR(g.856937G>A,g.922947A>G)and ESR1(g.190744A>G)were significantly associated with laying performance(P<0.05),but were not correlated with egg quality(P>0.05). The results suggest FSHR and ESR1 play an important role in early gonad development in Leizhou black duck,and three identified SNPs may be used as genotype maker for egg laying. %U https://biotech.aiijournal.com/EN/10.13560/j.cnki.biotech.bull.1985.2019-0094