蒙古沙冬青外向钾离子通道AmGORK启动子克隆及表达分析

李俊林, 张焕朝, 聂文婧, 张海洋, 王向誉, 郭洪恩, 韩蕾

Cloning and Expression Analysis of Outward Potassium Ion Channel Gene AmGORK Promoter from Ammopiptanthus mongolicus

LI Jun-lin, ZHANG Huan-chao, NIE Wen-jing, ZHANG Hai-yang, WANG Xiang-yu, GUO Hong-en, HAN Lei

表2 AmGORK启动子序列预测分析

Table 2 Prediction analysis of AmGORK promoter sequence

基序名称Site name 物种Species 序列Sequence 功能Function AAAC-motif 菠菜 Spinacia oleracea CAATCAAAACCT 光响应元件 Light-responsive element AACA-motif 水稻 Oryza sativa TAACAAACTCCA 胚乳特异性表达 Involved in endosperm-specific expression ABRE 拟南芥 A. thaliana TACGTG ABA响应顺式作用元件cis-acting element involved in the abscisic acid responses CAAT-box 拟南芥 A. thaliana GGCAAT 启动子和增强子区域共有顺式作用元件Common cis-acting element in promoter and enhancer regions G-box 金鱼草 Antirrhinum majus CACGTA 光反应中的顺式调节元件cis-acting regulatory element involved in light responses GAG-motif 大麦 Hordeum vulgare GGAGATG 光响应元件的一部分 Part of a light-responsive element GARE-motif 甘蓝 Brassica oleracea AAACAGA 赤霉素响应元件 Gibberellin-responsive element GCN4 水稻 O. sativa TGAGTCA 胚乳表达顺式调控元件 cis-regulatory element involved in endosperm expression HSE 甘蓝 B. oleracea AAAAAATTTC 热响应顺式作用元件 cis-acting element involved in heat stress responses I-box 豌豆 Pisum sativum ATGATATGA 光响应元件的一部分 Part of a light-responsive element LAMP-element 菠菜 S. oleracea CCTTATCCA 光响应元件的一部分 Part of a light-responsive element LTR 大麦 H. vulgare CCGAAA 低温响应顺式作用元件 cis-acting element involved in low-temperature responses MBS 拟南芥 A. thaliana TAACTG 干旱诱导MYB结合位点 MYB binding site involved in drought-inducibility MRE 荷兰芹 Petroselinum crispum AACCTAA MYB结合位点参与光响应 MYB binding site involved in light responses O2-site 玉米 Zea mays GATGACATGG 玉米蛋白代谢调控顺式作用元件 cis-acting regulatory element involved in zein metabolism regulation Skn-1 motif 水稻 Oryza sativa GTCAT 胚乳表达必需的顺式作用元件 cis-acting regulatory element required for endosperm expression Sp1 玉米 Zea mays CC（G/A）CCC 光响应元件 Light-responsive element TATA-box 拟南芥 A. thaliana TATA 在转录开始前30 bp核心启动子元件 Core promoter element around -30 bp of transcription start TC-rich repeats 烟草 Nicotiana tabacum ATTTTCTCCA 防御及胁迫响应顺式作用元件 cis-acting element involved in defense and stress responses TCT-motif 拟南芥 A.s thaliana TCTTAC 光响应元件的一部分 Part of a light-responsive element circadian 番茄 Lycopersicon esculentum CAANNNNATC 昼夜节律控制顺式作用元件 cis-acting regulatory element involved in circadian control rbcS-CMA7a 膨胀浮萍 Lemna gibba GTCGATAAGG 光响应元件的一部分 Part of a light-responsive element