具有辅助降解纤维素功能的大斑刚毛座腔菌糖苷水解酶GH61的鉴定、异源表达及功能分析

马玉倩, 孙东辉, 岳浩峰, 辛佳瑜, 刘宁, 曹志艳

Identification, Heterologous Expression and Functional Analysis of a GH61 Family Glycoside Hydrolase from Setosphaeria turcica with the Assisting Function in Degrading Cellulose

MA Yu-qian, SUN Dong-hui, YUE Hao-feng, XIN Jia-yu, LIU Ning, CAO Zhi-yan

表1 实时荧光定量PCR引物序列

Table 1 Primer sequences used in RT-qPCR

引物名称Primer name 序列Sequence（5'-3'） 引物名称Primer name 序列Sequence（5'-3'） StGH61_1-F GGCTCTGGAACTGGCAAGAT StGH61_12-F CTCCTGGTCCGTCACTGTTC StGH61_1-R TAGTACTCGAGGTCCCTGGC StGH61_12-R GGCTGGAGAGATCGGTCATG StGH61_2-F GTCAGTTTCCCGGGTGCTTA StGH61_13-F GGCAGAAGGACCAGATCGAG StGH61_2-R GACCACGCTGTTAGTCCCAA StGH61_13-R CGCTGTAGAGACCGGGAATC StGH61_3-F ACATCAACACGTGGGATCCC StGH61_14-F CGACGCCATCCTAGACACTC StGH61_3-R TGCGCACGACAGGTAGAATT StGH61_14-R TTGAAGTCGATGCCTGGGTC StGH61_4-F CGACTTCCGTTGCAACAAGG StGH61_15-F CACCAAAGTCGAGCCCTTCT StGH61_4-R AACATCACCAGGAGCCTTGG StGH61_15-R TTGAGGGAGAGGGAGACGAG StGH61_5-F TTGCGCAAATCACAACCTCG StGH61_16-F GTGCCAAGGGAGGTCTCTTC StGH61_5-R TCGTGGCATGTCATGTTGGA StGH61_16-R GGTCGGCAGTGAAAGAGACA StGH61_6-F CACCCAGACTGTCACCATCC StGH61_17-F GGTTCAAGGTGCAGGAGGAA StGH61_6-R CACCCAGACTGTCACCATCC StGH61_17-R CTACCCGTCACCTTGAGCTG StGH61_7-F GGTCCAAGTTCTCGCAGGAA StGH61_18-F AGCTCGACTGCCATGATCTG StGH61_7-R CATTCACATTCGAGCCGCTG StGH61_18-R GGGGAGCTTGACGTTGATGA StGH61_8-F CCACAAGAATGCTAGCCCCA StGH61_19-F CAAGGTCTCCAACGCAGCTA StGH61_8-R GCACCCTCAATCTTGGTCCA StGH61_19-R AAAATTGAGCACCGCCAACC StGH61_9-F TGGTTCAAGGTTTCCAGCGA StGH61_20-F CATCCCTGCTTGTATTGCGC StGH61_9-R AAATGTAGAATTGCGCGCCG StGH61_20-R GGAACGAGACGGTTGATGGT StGH61_10-F GGGCAGTGATGTGAAGAGCT StGH61_21-F AAGATCGACGAGCAAGGCAT StGH61_10-R GGAGCACGCGAGGTAGAATT StGH61_21-R GACGCATACCCAGGAATCGT StGH61_11-F AGCCTCTCTTCCTCGGACAT β-tubulin-F GTGCGCAAGGAGGCTGAGGG StGH61_11-R TTTTGGCGTCGCTGACTTTG β-tubulin-R CATGAAGAAATGGAGACGGGGGAA