具有辅助降解纤维素功能的大斑刚毛座腔菌糖苷水解酶GH61的鉴定、异源表达及功能分析

马玉倩, 孙东辉, 岳浩峰, 辛佳瑜, 刘宁, 曹志艳

Identification, Heterologous Expression and Functional Analysis of a GH61 Family Glycoside Hydrolase from Setosphaeria turcica with the Assisting Function in Degrading Cellulose

MA Yu-qian, SUN Dong-hui, YUE Hao-feng, XIN Jia-yu, LIU Ning, CAO Zhi-yan

表2 大斑刚毛座腔菌糖苷水解酶GH61家族蛋白性质预测

Table 2 Prediction of the protein properties of the glycoside hydrolase GH61 family from S. turcica

基因 Gene 检索号 Accession number 氨基酸数目 Number of amino acids 分子量 Molecular weight/kD 等电点 pI 信号肽长度 Signal peptide length/aa 蛋白定位 Protein location StGH61-1 XP_008027730 250 26.69 8.26 16 胞外Extracellular StGH61-2 XP_008031219 334 36.13 5.77 18 胞外Extracellular StGH61-3 XP_008024527 221 23.46 8.33 17 胞外Extracellular StGH61-4 XP_008025833 418 43.38 7.63 17 胞外Extracellular StGH61-5 XP_008021808 266 28.81 8.24 21 胞外Extracellular StGH61-6 XP_008023212 291 32.06 6.16 22 膜结合溶酶体Lysosomes StGH61-7 XP_008024957 248 26.32 7.69 20 胞外Extracellular StGH61-8 XP_008025253 329 34.56 5.98 22 分泌到胞外 Extracellular（Secreted） StGH61-9 XP_008030985 149 15.76 7.83 — 分泌到胞外 Extracellular（Secreted） StGH61-10 XP_008022828 236 25.55 9.00 20 膜结合线粒体Mitochondria StGH61-11 XP_008023697 229 23.52 8.49 16 胞外Extracellular StGH61-12 XP_008023696 258 27.49 5.47 22 胞外Extracellular StGH61-13 XP_008028494 325 34.02 6.31 18 胞外Extracellular StGH61-14 XP_008028319 292 30.73 6.28 21 胞外Extracellular StGH61-15 XP_008025489 472 49.64 7.44 19 胞外Extracellular StGH61-16 XP_008027303 222 23.26 7.69 20 胞外Extracellular StGH61-17 XP_008027295 246 25.92 8.44 18 胞外Extracellular StGH61-18 XP_008024355 344 34.75 6.49 17 胞外Extracellular StGH61-19 XP_008022590 243 25.06 8.90 17 胞外Extracellular StGH61-20 XP_008026441 233 24.21 7.67 18 胞外Extracellular