具有辅助降解纤维素功能的大斑刚毛座腔菌糖苷水解酶GH61的鉴定、异源表达及功能分析

马玉倩, 孙东辉, 岳浩峰, 辛佳瑜, 刘宁, 曹志艳

Identification, Heterologous Expression and Functional Analysis of a GH61 Family Glycoside Hydrolase from Setosphaeria turcica with the Assisting Function in Degrading Cellulose

MA Yu-qian, SUN Dong-hui, YUE Hao-feng, XIN Jia-yu, LIU Ning, CAO Zhi-yan

图2 大斑刚毛座腔菌纤维素酶的滤纸酶活及GH61家族基因表达分析 A：大斑刚毛座腔菌纤维素酶的滤纸酶活；B：大斑刚毛座腔菌中GH61家族糖苷水解酶基因的表达图谱；C：RT-qPCR验证大斑刚毛座腔菌中GH61家族糖苷水解酶基因相对表达量。不同小写字母表示同一基因在不同处理下存在显著差异（P<0.05）

Fig. 2 Filter paper enzymatic activity and GH61 family gene expression analysis of cellulase from S. turcica A: Filter paper enzymatic activity of cellulase from S. turcica. B: Expression map of GH61 family glycoside hydrolase genes in S. turcica. C: RT-qPCR verifies the relative expression of GH61 family glycoside hydrolase genes in S. turcica. Different normal letters indicate significant differences among different treatments at 0.05 level