CRISPR相关转座酶及其细菌基因组编辑应用

周晓杰, 杨思琪, 张译文, 徐佳琪, 杨晟

CRISPR-associated Transposases and Their Applications in Bacterial Genome Editing

ZHOU Xiao-jie, YANG Si-qi, ZHANG Yi-wen, XU Jia-qi, YANG Sheng

表1 不同亚型CASTs在细菌中的部署

Table 1 Implementation of different subtype CASTs in bacteria

亚型 Subtype 缩写或转座子编号 Abbreviation or transposon No. 来源 Source 底盘 Chassis 最高效率 Highest efficiency 参考文献 Reference I-F VchCAST Vibrio cholerae Tn6677 Escherichia coli ~100% [41] Tatumella citrea ~100% [60] Klebsiella oxytoca N.D.* [41] Pseudomonas putida N.D.* [41] Klebsiella michiganensis ~0.010 [64] Pseudomonas simiae ~0.003 [64] Ralstonia sp. UNC404CL21Col ~0.001 [64] PtrCAST Pseudoalteromonas translucida KMM520 Escherichia coli ~100% [34] AsaCAST Aeromonas salmonicidaS44 Escherichia coli 33.4% **&*** [35] Tn7000 Vibrio cholerae 4874 Escherichia coli ~1% [36] Tn7001 Photobactenium iliopiscarium NCIMB 13355 Escherichia coli ~1% [36] Tn7002 Vibrio sp.F12 Escherichia coli ~0.2% [36] Tn7003 Vibrnio parahaemolyticusFORC 071 Escherichia coli ~2% [36] Tn7004 Vibrio sp.16 Escherichia coli ~0.05% [36] Tn7005 Vibrio cholerae M1517 Escherichia coli ~45% [36] Tn7006 Vibrio splendidus UCD-SED10 Escherichia coli ~0.4% [36] Tn7007 Alivibrio wodanis06/09/160 Escherichia coli ~40% [36] Tn7008 Alivibrio sp.1S175 Escherichia coli ~0.13% [36] Tn7009 Parashewanella spongiaeHJ039 Escherichia coli ~50% [36] Tn7010 Photobacterium ganghwense JCM 12487 Escherichia coli ~0.4% [36] Tn7011 Pseudoalteromonas sp.P1-25 Escherichia coli ~48% [36] Tn7012 Pseudoalteromonas ruthenica S3245 Escherichia coli ~5% [36] Tn7013 Vibrio cholerae OYP7G04 Escherichia coli ~0.1% [36] Tn7014 Vibnio diazotrophicus60.6F Escherichia coli ~30% [36] Tn7015 Shewanella sp.UCD-KL21 Escherichia coli ~6% [36] Tn7016 Pseudoalteromonassp.S983 Escherichia coli ~80% [36] Tn7017 Endozoicomonas ascidicolaAVMARTO5 Escherichia coli ~16% [36] I-B AvCAST Anabaena variabilis Escherichia coli 2.50% [37] PmcCAST Peltigera membranacea cyanobiont 210A Escherichia coli 0.85% [37] RoCAST Rippkaea orientalis Escherichia coli N.D.* [38] V-K ShCAST Scytonema hofmanni Escherichia coli 80% [27] Sinorhizobium meliloti ~100% [62] Shewanella oneidensis MR-1 100% [61] Burkholderia thailandensis ~100% ** [63] Pseudomonas putida ~100% ** [63] Agrobacterium fabrum Anabaena 40% ** N.D.* [63] [65] ShoCAST Scytonema hofmannii PCC 7110 Escherichia coli 40% [41] AcCAST Anabaena cylindrica / N.D.* [27] ShHELIX Scytonema hofmanni Escherichia coli ~80% [59] ShoHELIX Scytonema hofmannii PCC 7110 Escherichia coli ~50% [59] AcHELIX Anabaena cylindrica Escherichia coli ~90% [59]