新型GH5家族多结构域纤维素酶的结构与功能研究
杨俊钊, 张新蕊, 赵国柱, 郑菲

Structure and Function Analysis of Novel GH5 Multi-domain Cellulase
YANG Jun-zhao, ZHANG Xin-rui, ZHAO Guo-zhu, ZHENG Fei
图3 野生型TlCel5、ReCel5和突变体TM1、TM2的SDS-PAGE分析
M:蛋白分子质量标准; A:野生型TlCel5、ReCel5的表达(1:ReCel5纯化蛋白;2:TlCel5纯化蛋白); B:突变体TM1、TM2的表达(3:突变体TM1纯化蛋白;4:突变体TM2纯化蛋白)
Fig. 3 SDS-PAGE analysis of wild-type TlCel5, ReCel5 and mutant TM1, TM2
M: Protein molecular weight standard. A: Expression of wile-type TlCel5、ReCel5(1: purified protein of ReCel5; 2: purified protein of TlCel5). B: Expressions of mutants TM1 and TM2(3: purified protein of TM1; 4: purified protein of TM2)