一株高效驱油菌株HB-2降解原油活性的研究

生物技术通报 ›› 2013, Vol. 0 ›› Issue (11): 170-175.

一株高效驱油菌株HB-2降解原油活性的研究

游靖^1，2, 李青², 刘洋³, 吴刚², 王冠², 赖丰利², 李勇斌², 段丽莎², 曹艳花³

1.华东理工大学，上海 200237；2.华北油田采油工程研究院，任丘 062552；3.中国食品发酵工业研究院中国工业微生物菌种保藏管理中心，北京 100027

收稿日期:2013-04-24 出版日期:2013-11-14 发布日期:2013-11-14
作者简介:游靖，男，高级工程师，研究方向：三次采油；E-mail：cyy_youjing@petrochina.com.cn
基金资助:
中国石油天然气股份有限公司华北油田分公司重大科技项目(2012-HB-z231)，中国食品发酵工业研究院科技发展基金(博士基金)项目(2012KJFZ-BS-01)

Activities of an Oil Degrading Bacterial Strain HB-2

You Jing^1，2, Li Qing², Liu Yang³, Wu Gang², Wang Guan², Lai Fengli², Li Yongbin², Duan Lisha², Cao Yanhua³

(1. East China University of Science and Technology，Shanghai 200237； 2. Petroleum Production Engineering Research Institute of Huabei Oilfield Company，Renqiu 062552； 3. China Center of Industrial Culture Collection，China National Research Institute of Food and Fermentation Industries, Beijing 100027)

Received:2013-04-24 Published:2013-11-14 Online:2013-11-14

摘要/Abstract

摘要： 以原油为唯一碳源，从华北油田油井产出液中分离筛选得到一株高效降解原油菌株HB-2，经本研究组前期的多相分类鉴定，已将其确定为藤黄色单胞菌(Luteimonas)属中的一个新种华北藤黄色单胞菌(Luteimonas huabeiensis)。该菌与原油作用后，降黏率达47.3%，降低油水界面张力38.7%，能代谢一定量的有机酸和表面活性剂，通过原油组分分析，使原油轻质组分增加，有效地改善了原油的流动性，通过室内物模试验，提高采收率高达15.4%。

关键词: 油井产出液, 华北藤黄色单胞菌(Luteimonas huabeiensis), 乳化降黏, 物模试验

Abstract: An oil degrading strain HB-2 was isolated from the produced liquid of oil wells in Huabei Oilfield, which used crude oil as sole carbon. The strain was identified as a new species of the genus Luteimonas by the method of polyphasic identification. Through the reaction with crude oil, we find the viscosity-reducing rate is 47.3% and the oil-water interfacial tension decrease by 38.7%, in addition, the strain can also produce organic acid and surfactant. According to analyzing components of crude oil, we find the strain can improve the fluidity of crude oil by increasing light component of oil. According to the result of indoor model test, we can find the recovery ratio is increased by 15.4%.

Key words: Produced liquid of oil wells, Luteimonas huabeiensis, Emulsion visbreaking, Indoor model test

游靖, 李青, 刘洋, 吴刚, 王冠, 赖丰利, 李勇斌, 段丽莎, 曹艳花. 一株高效驱油菌株HB-2降解原油活性的研究[J]. 生物技术通报, 2013, 0(11): 170-175.

You Jing, Li Qing, Liu Yang, Wu Gang, Wang Guan, Lai Fengli, Li Yongbin, Duan Lisha, Cao Yanhua. Activities of an Oil Degrading Bacterial Strain HB-2[J]. Biotechnology Bulletin, 2013, 0(11): 170-175.