生物技术通报 ›› 2017, Vol. 33 ›› Issue (10): 97-102.doi: 10.13560/j.cnki.biotech.bull.1985.2017-0417

• 综述与专论 • 上一篇    下一篇

漆酶可降解底物种类的研究进展

张泽雄, 刘红艳, 邢贺, 马钰   

  1. 桂林理工大学化学与生物工程学院,桂林 541004
  • 收稿日期:2017-05-22 出版日期:2017-10-29 发布日期:2017-10-29
  • 作者简介:张泽雄,男,硕士研究生,研究方向:环境微生物化学;E-mail:2542979281@qq.com
  • 基金资助:
    国家自然科学基金项目(21467006)

Research Progress on Substrate Species Degraded by Laccase

ZHANG Ze-xiong, LIU Hong-yan, XING He, MA Yu   

  1. College of Chemistry and Bioengineering,Guilin University of Technology,Guilin 541004
  • Received:2017-05-22 Published:2017-10-29 Online:2017-10-29

摘要: 漆酶是一种含铜离子的多酚氧化酶,广泛存在于植物及真菌中。漆酶含特有的铜离子,其功能为传递结构中的电子,使漆酶具有了较强的氧化还原能力,能与木质素、胺类化合物、芳香化合物等底物发生作用,且大多数反应的唯一产物为水。目前,漆酶在降解多种有毒物质和有害污染物方面表现出高效、成本较低的特性,如白腐真菌所产的高水平漆酶已广泛成熟应用在工业废水处理等生物整治和修复领域。近年来最新研究利用载体固定化酶的技术使漆酶能够在使用后回收反复利用且更具有稳定性,这降低成本的同时还保持了漆酶催化氧化的特性,克服了不少漆酶在解决环境污染中出现的问题。利用介体的介导作用解决了漆酶氧化还原电势较低的问题,大量增多了可降解底物的种类,使其在废水处理、污染物降解、土壤修复、工业染料漂白等领域的应用前景更广阔。对现有漆酶应用于各领域进行研究总结,综述了降解各领域中的有害污染物等底物种类,提出了利用漆酶的降解过程中的现有不足和改进方向,以期为生物法降解环境污染物的研究提供参考。

关键词: 漆酶, 底物, 环境污染物

Abstract: Laccase,a polyphenol oxidase that binds multiple copper ions,is widely found in plants and fungi. Laccase contains unique copper ions which function is to transfer electrons in the structure,so that the laccase has a strong redox capacity,and the only product of the most reactions with lignin,amine compounds,aromatic compounds such as the role of the substrate is water. It has the characteristics of high efficiency and low cost in the degradation of many toxic substances and harmful pollutants,such as white rot fungi produced by the high level of laccase has been widely used in industrial wastewater treatment and other biological remediation and repair areas. In recent years,the lastest research showed that the using of vector immobilized enzyme technology allows laccase to be recycled after use and reused and more stable,which reduces costs while maintains the characteristics of laccase catalytic oxidation,it has overcome the problems of laccases in solving environmental pollution,Under the mediating effect of mediator,the problem of low potential of laccase redox has been figured out,and it greatly increased more kinds of substrate. Therefore,the application prospects in wastewater,degradation,soil remediation,industrial bleaching and other fields will be much broader. This paper summarily describes the applications of laccase in various fields and the types of harmful pollutions substrates,put forward the deficiency and the improving direction of existing degradation process of laccase aiming at providing the reference for the study of biodegradation of environmental pollutants.

Key words: laccase, substrate, environmental pollutant