致病疫霉RXLR效应蛋白相关研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2017-0981

摘要/Abstract

摘要： 由致病疫霉（Phytophthora infestans（Mont.）de Bary）引起的晚疫病是马铃薯生产中最具毁灭性的病害。为了成功入侵和在寄主植物中繁衍,致病疫霉会向寄主细胞分泌一类RXLR效应蛋白以干扰植物免疫系统。自2005年克隆第一个晚疫病菌RXLR类无毒基因AVR3a以来,国内外学者从RXLR效应蛋白的结构、功能,以及与寄主靶标作用机理等多个方面展开了大量研究。随着高通量测序技术与效应子组学技术的发展,RXLR效应蛋白抑制植物免疫分子机制也取得了显著进展。RXLR效应蛋白的研究有助于揭示致病疫霉与马铃薯互作分子机制,并进一步为马铃薯抗病育种工作提供新思路。主要概述了致病疫霉RXLR效应蛋白的相关研究进展,重点介绍了致病疫霉AVR基因的克隆、定位、变异及功能等方面的最新进展,同时对未来值得关注的研究方向进行了探讨。

关键词: 致病疫霉, RXLR效应蛋白, 无毒基因, 晚疫病

Abstract: Late blight caused by Phytophthora infestans（Mont.）de Bary is one of the most destructive diseases for potatoes. To successful infect and colonize host plant,P. infestans secretes many RXLR effector proteins into plant cells to disturb plant’s immune responses. Researchers have paid much attentions on the RXLR protein structure,function and RXLR protein-target interaction investigations since the first P. infestans RXLR gene AVR3a was cloned in 2005. With the development of high-throughput sequencing technology and effectoromics technology,the molecular mechanisms by which these RXLR effectors suppress plant immune responses have been greatly progressed. The recent convergence of RXLR effector proteins,leading a better understanding on the molecular mechanism of P. infestans-potato interaction,will provide perspectives for future potato disease resistance and breeding. Here we review the research progress on P. infestans RXLR effector proteins,especially focusing on the new findings in cloning,localization,variation and functional mechanism of AVR genes. We further discuss several important research directions on RXLR effectors in the future.

Key words: Phytophthora infestans, RXLR effector protein, avirulence gene, late blight

王洪洋, 秦丽娟, 唐唯, 田振东. 致病疫霉RXLR效应蛋白相关研究进展[J]. 生物技术通报, 2018, 34(2): 102-111.

WANG Hong-yang, QIN Li-juan, TANG Wei, TIAN Zhen-dong. Research Advances on Phytophthora infestans RXLR Effector Proteins[J]. Biotechnology Bulletin, 2018, 34(2): 102-111.

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