农业转基因核酸标准物质研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2021-1495

摘要/Abstract

摘要：

转基因作物自商业化以来,产量和种植面积持续增加。为了对转基因农作物及其产品进行更规范的管理,保护消费者知情权和选择权,需要对转基因产品进行检测,转基因有证标准物质为转基因农作物及产品的检测提供标准化量值参考,是保障转基因作物及产品检测结果准确一致的重要基础。本文基于转基因检测标准物质的重要性,查阅和总结了国内外已经获批的农业转基因检测标准物质情况,并介绍了转基因检测标准物质的研制过程,比较了常用的3种不同类型的转基因检测标准物质,梳理了转基因检测标准物质研制中所存在的问题,并针对这些问题就我国转基因检测标准物质研制提出建议,以进一步促进我国农业转基因检测标准物质的研究和应用。

关键词: 转基因农作物, 有证标准物质, 标准化, 研制

Abstract:

Since the commercialization of genetically modified crops,the yield and planting area of it have increased continuously. In order to carry out more standardized management of genetically modified crops and their products,and to protect consumers’ right of knowing and choosing,it is necessary to detect genetically modified products. Genetically modified reference materials provide standardized reference for the detection of genetically modified crops and their products,which is an important basis for ensuring accuracy and consistency of test result of genetically modified crops and their products. In this paper,the certified reference materials of agricultural genetically modified crops at home and abroad are reviewed and summarized,the development of genetically modified reference materials is introduced,three common types of genetically modified reference materials are compared,issues in the development of genetically modified reference materials are sorted and suggestions also are put forward,aiming to promote the research and application of agricultural genetically modified reference materials in China.

Key words: genetically modified crops, certified reference materials, standardization, development

林鹰, 杨文莉, 周玲艳, 姜大刚. 农业转基因核酸标准物质研究进展[J]. 生物技术通报, 2022, 38(8): 52-59.

LIN Ying, YANG Wen-li, ZHOU Ling-yan, JIANG Da-gang. Research Progress in Agricultural Genetically Modified Nucleic Acid Reference Materials[J]. Biotechnology Bulletin, 2022, 38(8): 52-59.

图/表 7

参考文献 38

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研发机构 Research institution	标准物质名称 Name of reference materials	转化体名称Transgenic event	转化性状 Transgenic character	规格 Norms	价格 Price/¥
美国油脂化学家学会	T45 Leaf Tissue DNA	T45	抗草铵膦除草剂	10 µg	1 692.69
	Topas 19/2 Leaf Tissue DNA	Topas 19/2	抗草铵膦除草剂、抗生素抗性	10 µg	1724.3
	MON87411 Powder	MON87411	抗草甘膦除草剂、抗虫	10 g	757.26
	MON88701 Powder	MON88701	抗草铵膦和麦草畏除草剂	10 g	757.26
欧盟联合研究中心	Bt11 MAIZE（nominal 100% GMO）	Bt11	抗虫	-	426.86
	DAS-81910-7 COTTON（nominal 100% GMO）	DAS-81910-7	抗除草剂2、4-二氯甲氧乙酸	-	504.47
	SOYA 305423（lea 1-nominal 0.5% GMO）	305423S	抗对乙酰乳酸合酶抑制除草剂	-	465.67
	73496 RAPESEED（nominal 100% GMO）	73496	抗草甘膦除草剂	-	520.00
	AV43-6-G7 POTATO（nominal 100% GMO）	AV43-6-G7	工业合成淀粉	-	613.13

研发机构 Research institution	标准物质名称 Name of reference materials	转化体名称Transgenic event	转化性状 Transgenic character	规格 Norms	价格 Price/¥
美国油脂化学家学会	T45 Leaf Tissue DNA	T45	抗草铵膦除草剂	10 µg	1 692.69
	Topas 19/2 Leaf Tissue DNA	Topas 19/2	抗草铵膦除草剂、抗生素抗性	10 µg	1724.3
	MON87411 Powder	MON87411	抗草甘膦除草剂、抗虫	10 g	757.26
	MON88701 Powder	MON88701	抗草铵膦和麦草畏除草剂	10 g	757.26
欧盟联合研究中心	Bt11 MAIZE（nominal 100% GMO）	Bt11	抗虫	-	426.86
	DAS-81910-7 COTTON（nominal 100% GMO）	DAS-81910-7	抗除草剂2、4-二氯甲氧乙酸	-	504.47
	SOYA 305423（lea 1-nominal 0.5% GMO）	305423S	抗对乙酰乳酸合酶抑制除草剂	-	465.67
	73496 RAPESEED（nominal 100% GMO）	73496	抗草甘膦除草剂	-	520.00
	AV43-6-G7 POTATO（nominal 100% GMO）	AV43-6-G7	工业合成淀粉	-	613.13

研发机构 Research institution	标准品名称 Name of reference materials	转化事件 Transgenic event	转化性状 Transgenic character	规格 Norms	价格 Price/¥
中国计量科学研究院	转基因BT63水稻种子粉基体标准物质	BT63	抗虫	1 g	3 600
	转基因水稻BT63质粒分子标准物质	BT63	抗虫	500 μL	720
	转基因大豆MON89788质粒分子标准物质	MON89788	-	500 μL	720
中国农业科学院油料作物研究所,农业部科技发展中心,中国计量科学研究院	转基因玉米双抗12-5基因组DNA标准物质	双抗12-5	双价抗虫	100 μL	-
中国农业科学院油料作物研究所,农业农村部科技发展中心,中国农业科学院生物技术研究所	转基因水稻G6H1基因组DNA标准物质	G6H1	抗虫耐除草剂	100 μL	-
	转基因水稻TT51-1基因组DNA标准物质	TT51-1	抗虫	100 μL	-
	转基因水稻科丰6号基因组DNA标准物质	科丰6号	双价抗虫	-	-
	转基因水稻克螟稻基因组DNA标准物质	克螟稻	抗虫	-	-
农业部科技发展中心,中国农业科学院油料作物研究所	转基因大豆MON89788基体标准物质	MON89788	抗草甘膦除草剂	-	-
农业农村部科技发展中心,中国农业科学院生物技术研究所,上海交通大学	转基因水稻G6H1基体标准物质	G6H1	抗虫、耐除草剂	-	-

研发机构 Research institution	标准品名称 Name of reference materials	转化事件 Transgenic event	转化性状 Transgenic character	规格 Norms	价格 Price/¥
中国计量科学研究院	转基因BT63水稻种子粉基体标准物质	BT63	抗虫	1 g	3 600
	转基因水稻BT63质粒分子标准物质	BT63	抗虫	500 μL	720
	转基因大豆MON89788质粒分子标准物质	MON89788	-	500 μL	720
中国农业科学院油料作物研究所,农业部科技发展中心,中国计量科学研究院	转基因玉米双抗12-5基因组DNA标准物质	双抗12-5	双价抗虫	100 μL	-
中国农业科学院油料作物研究所,农业农村部科技发展中心,中国农业科学院生物技术研究所	转基因水稻G6H1基因组DNA标准物质	G6H1	抗虫耐除草剂	100 μL	-
	转基因水稻TT51-1基因组DNA标准物质	TT51-1	抗虫	100 μL	-
	转基因水稻科丰6号基因组DNA标准物质	科丰6号	双价抗虫	-	-
	转基因水稻克螟稻基因组DNA标准物质	克螟稻	抗虫	-	-
农业部科技发展中心,中国农业科学院油料作物研究所	转基因大豆MON89788基体标准物质	MON89788	抗草甘膦除草剂	-	-
农业农村部科技发展中心,中国农业科学院生物技术研究所,上海交通大学	转基因水稻G6H1基体标准物质	G6H1	抗虫、耐除草剂	-	-

标准编号 Standard code	标准名称 Name of standard
农业部1782号公告-8-2012	转基因植物及其产品成分检测基体标准物质制备技术规范
农业部1782号公告-9-2012	转基因植物及其产品成分检测标准物质试用评价技术规范
农业部2259号公告-1-2015	转基因植物及其产品成分检测基体标准物质定值技术规范
农业部2259号公告-2-2015	转基因植物及其产品成分检测玉米标准物质候选物繁殖与鉴定技术规范
农业部2259号公告-3-2015	转基因植物及其产品成分检测棉花标准物质候选物繁殖与鉴定技术规范
农业农村部公告第111号-1-2018	转基因植物及其产品成分检测基因组DNA标准物质制备技术规范
农业农村部公告第111号-2-2018	转基因植物及其产品成分检测基因组DNA标准物质定值技术规范
农业农村部公告第323号-6-2020	转基因植物及其产品成分检测油菜标准物质原材料繁殖与鉴定技术规范
农业农村部公告第323号-7-2020	转基因植物及其产品成分检测大豆标准物质原材料繁殖与鉴定技术规范
农业农村部公告第323号-8-2020	转基因植物及其产品成分检测质粒DNA标准物质制备技术规范
农业农村部公告第323号-22-2020	转基因植物及其产品成分检测水稻标准物质原材料繁殖与鉴定技术规范