生物技术通报 ›› 2022, Vol. 38 ›› Issue (1): 51-69.doi: 10.13560/j.cnki.biotech.bull.1985.2021-1205

• 特约综述 • 上一篇    下一篇

从功能基因到生物学性状:大丽轮枝菌致病性形成的分子基础

田李1(), 李俊娇2, 戴小枫2, 张丹丹2, 陈捷胤2()   

  1. 1.曲阜师范大学生命科学学院,济宁 273165
    2.中国农业科学院植物保护研究所,北京 100193
  • 收稿日期:2021-09-18 出版日期:2022-01-26 发布日期:2022-02-22
  • 通讯作者: 陈捷胤
  • 作者简介:田李,男,博士,副教授,研究方向:植物病理学;E-mail: biotian@qfnu.edu.cn
  • 基金资助:
    国家自然科学基金项目(31972228);国家自然科学基金项目(31501588);国家自然科学基金项目(31970142);山东省自然科学基金(ZR2020MC115)

From Functional Genes to Biological Characteristics:The Molecular Basis of Pathogenicity in Verticillium dahliae

TIAN Li1(), LI Jun-jiao2, DAI Xiao-feng2, ZHANG Dan-dan2, CHEN Jie-yin2()   

  1. 1. College of Life Science,Qufu Normal University,Jining 273165
    2. State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193
  • Received:2021-09-18 Online:2022-01-26 Published:2022-02-22
  • Contact: CHEN Jie-yin

摘要:

大丽轮枝菌为典型的土传维管束病原真菌,针对其致病相关基因的挖掘与功能解析一直是植物病理学研究的热点。大丽轮枝菌具有定殖维管束、“毒素”致萎、形成微菌核、种群分化多元化等特征,这些性状最终支撑或决定了病原对寄主的致病性基础。从进化角度来说,功能基因决定生物学性状。截至目前,在大丽轮枝菌中已鉴定出上百个功能基因;但针对其与病原的重要生物学性状和致病性之间的关系尚未系统阐述。为此,本文概述了大丽轮枝菌功能基因鉴定的发展历程,并以功能基因决定生物学性状为逻辑线条,重点梳理了功能基因与大丽轮枝菌重要生物学性状形成的关系,厘清了大丽轮枝菌致病性与决定重要生物学性状功能基因的关系。这些观点将为系统建立大丽轮枝菌重要生物学性状形成的功能基因网络,以及从功能基因决定生物学性状角度阐明大丽轮枝菌致病形成的分子基础提供理论支撑。

关键词: 黄萎病, 大丽轮枝菌, 生物学性状, 功能基因, 致病性

Abstract:

Verticillium dahliae is a soil-borne vascular pathogen fungus,excavation and functional analysis of its disease-related genes has been a hotspot in plant pathology research. V. dahliae is characterized of adapting to the plant vascular niche,of inducing foliar wilting either by producing toxins or employing its secretome,of forming microsclerotia for long-term survival,and of evolving a variety of population structures,etc.,thus these traits ultimately support or determine the pathogenic basis of pathogenicity to the host. Evolutionarily,functional genes determine the biological characteristics of a pathogen. Currently,hundreds of functional genes have been identified in V. dahliae,but their relationships to the key biological characteristics and how they contribute to its virulence have remained an unexplored topic. We systematically outlined the development process of identifying the functional genes in V. dahliae,focused on sorting the relationships between pathogenicity of V. dahliae and the functional genes determining its key biological traits based on the logic clue of functional genes determining biological traits,thus clarified the relationships between pathogenicity of V. dahliae and the functional genes determining its key biological traits. This exploration will provide the theoretical foundations for systematically constructing the functional gene networks of key biological characteristics in V. dahliae and explaining the molecular basis of pathogenicity and virulence in V. dahliae from the aspect of the functional genes determining its key biological traits.

Key words: Verticillium-wilt, Verticillium dahliae, biological characteristic, functional gene, virulence pathogenicity