Abstract: In this study,the Reverse Transcription PCR（RT-PCR）was used to clone full-length sequence of FGF21（Fibroblast growth factor）gene from grass carp liver,and the quantitative real-time PCR（qPCR）was used to detect the expression levels of FGF21 gene in different juvenile tissues. Sequence analysis revealed that the full-length of cDNA of the FGF21 gene（GenBank accession number：MF_094727）was 615 bp,containing 51 bp 5'-untranslated region,564 bp open reading frame（ORF）,and encoding 187 amino acids. Alignment analysis showed that the amino acid sequence of FGF21 gene in grass carp were 78.86%,77.66%,73.94%,53.29%,52.41%,43.71%,35.96%,31.87%,and 30.39% identical to that from zebrafish（Danio rerio）,horned golden-line barbel（Sinocyclocheilus rhinocerous）,common carp（Cyprinus carpio）,rainbow trout（Oncorhynchus mykiss）,Atlantic salmon（Salmo salar）,Nile tilapia（Oreochromis niloticus）,Japanese medaka（Oryzias latipes）,human（Homo sapiens）,and house mouse（Mus musculus）,respectively. qPCR analysis indicated that the FGF21 gene expressed in all examined tissues including liver,foregut,midgut,hindgut,heart,muscle,kidney,and brain,and the highest in muscle,foregut,and midgut. These results indicate that the FGF21 gene may play an important role in muscle and intestine of grass carp.

Key words: grass carp（Ctenopharyngodon idellus）, FGF21 gene, gene clone, expression analysis