Isolation of Specific Phage of Ralstonia solanacearum and Its Effects on Control of Soil-borne Bacterial Wilt Disease

doi:10.13560/j.cnki.biotech.bull.1985.2020-0816

Abstract

Abstract: Soil-borne Ralstonia solanacearum is one of the most serious bacterial diseases,and phage,a virus specifically infecting bacteria,plays an important role in the control of R. solanacearum. Previous studies revealed that R. solanacearum in different regions have high polymorphism,while the phage is highly specific to host and its infection to R. solanacearum in the same region is strong. This study intends to in situ screen efficient and stable phage against R. solanacearum in a specific region,and to explore its antibacterial stability. A strain of R. solanacearum isolated from the Kirin Greenhouse in Nanjing was used as the host to screen its obligate phage from the diseased soil in the same area. A phage NJ-P3 with the strongest bacteriostatic ability was screened from it,its basic biological characteristics,stability and best preservation methods were tested indoors,and pot experiment to explore its optimal application methods. The phage NJ-P3 was a lytic podoviridae and there was a special protein in the genome that may be related to its strong lysis. Phage NJ-P3 had strong resistance,and tolerated to high temperature of 50℃,4-10 of pH,and 80 min of ultraviolet irradiation. NJ-P3 stored in SM buffer showed the highest stability in low temperature but can be stored in short time under normal temperature condition. Pot experiment demonstrated that the control effect on the soil-borne R. solanacearum was better compared to traditional soil drench（SD）method and increased by 37.5% on the average by the stem injection（SI）method for inoculating phage NJ-P3. In sum,we obtained a highly efficient and stable R. solanacearum-specific phage,and systematically explored the optimal storage conditions and application methods,which lays a theoretical foundation for the establishment of phage resource library of R. solanacearum and widespread application of phage in the prevention and control of soil-borne diseases in the future.

Key words: soil-borne Ralstonia solanacearum, specific phages, efficiency and stability, application methods

WANG Xiao-fang, HOU Yu-gang, YANG Ke-ming, WANG Jia-ning, WEI Zhong, XU Yang-chun, SHEN Qi-rong. Isolation of Specific Phage of Ralstonia solanacearum and Its Effects on Control of Soil-borne Bacterial Wilt Disease[J]. Biotechnology Bulletin, 2020, 36(9): 194-201.

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