重组骆驼蓬脂转移蛋白与顺铂联合抑制黑色素瘤B16细胞增殖效应的研究

doi:10.13560/j.cnki.biotech.bull.1985.2015.12.027

生物技术通报 ›› 2015, Vol. 31 ›› Issue (12): 186-192.doi: 10.13560/j.cnki.biotech.bull.1985.2015.12.027

重组骆驼蓬脂转移蛋白与顺铂联合抑制黑色素瘤B16细胞增殖效应的研究

常晨晨,王燕,管珊,孙素荣

新疆大学生命科学与技术学院新疆生物资源基因工程重点实验室，乌鲁木齐 830046

收稿日期:2015-05-27 出版日期:2015-12-19 发布日期:2015-12-19
作者简介:常晨晨，女，硕士研究生，研究方向:分子克隆与药物筛选；E-mail:528193074@qq.com
基金资助:
新疆维吾尔自治区科技支疆项目(20091129)，新疆生物资源基因工程重点实验室开放基金项目(XJDX0201-2010-06)

The Synergistic Inhibitory Effects of Recombinant Peganum Harmala Lipid Transfer Protein and Cisplatin on the Proliferation of Melanoma B16 Cells in vitro

Chang Chenchen, Wang Yan, Guan Shan, Sun Surong

Xinjiang Key Laboratory of Biological Resources and Genetic Engineering，College of Life Science and Technology，Xinjiang University，Urumqi 830046

Received:2015-05-27 Published:2015-12-19 Online:2015-12-19

摘要/Abstract

摘要： 旨在探讨重组骆驼蓬脂转移蛋白(Recombinant Peganum harmala lipid transfer protein，rPhLTP)与顺铂(DDP)联用对鼠黑色素瘤B16细胞增殖抑制和凋亡诱导作用。MTT法检测rPhLTP、顺铂单独及联用后对鼠黑色素瘤B16细胞增殖的影响；流式细胞术检测细胞凋亡，活性氧(Reactive oxygen species，ROS)和线粒体膜电位(Δψm)的变化情况。结果显示，rPhLTP和DDP联用后细胞生长抑制率和凋亡率显著高于DDP单独处理组(P < 0.01)；而且胞内ROS升高的水平均高于单独处理组(P < 0.01)，胞内Δψm水平均低于单独处理组，但无显著性差异。rPhLTP可增强顺铂对B16细胞的生长抑制并促进细胞凋亡，与顺铂具有协同作用。

关键词: 重组骆驼蓬脂转移蛋白, 顺铂, 鼠黑色素瘤B16, 增殖抑制

Abstract: This work is to explore how the combination of recombinant Peganum harmala lipid transfer protein(rPhLTP)and cisplatin(DDP)inhibits the proliferation of melanoma B16 cells and induces the apoptosis of them. MTT assay was used to measure the effects of individual rPhLTP and DDP and combination of them on the proliferation of melanoma B16 cells. The cell apoptosis rate, reactive oxygen species(ROS), and mitochondrial transmembrane potential(Δψm)level were measured using flow cytometry. The results showed that both inhibitory rate and apoptosis rate by the combination of rPhLTP and DDP were significantly higher(P < 0.01)than by individual DDP or rPhLTP, moreover also levels of ROS raised inside cells by the combination were higher than by individuals(P < 0.01). However, the levels of Δψm inside the cells by the combination were lower than those by individuals, but the differences between the combination and individuals were not such significant. In conclusion, rPhLTP enhanced the inhibitory effect of DDP on the proliferation of B16 cells and promoted the cell apoptosis, indicating that rPhLTP and DDP has synergistic antitumor effect on B16 cells.

Key words: recombinant peganum harmala lipid transfer protein, cisplatin, melanoma B16 cells, proliferation inhibition

常晨晨,王燕,管珊,孙素荣. 重组骆驼蓬脂转移蛋白与顺铂联合抑制黑色素瘤B16细胞增殖效应的研究[J]. 生物技术通报, 2015, 31(12): 186-192.

Chang Chenchen, Wang Yan, Guan Shan, Sun Surong. The Synergistic Inhibitory Effects of Recombinant Peganum Harmala Lipid Transfer Protein and Cisplatin on the Proliferation of Melanoma B16 Cells in vitro[J]. Biotechnology Bulletin, 2015, 31(12): 186-192.

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重组骆驼蓬脂转移蛋白与顺铂联合抑制黑色素瘤B16细胞增殖效应的研究

The Synergistic Inhibitory Effects of Recombinant Peganum Harmala Lipid Transfer Protein and Cisplatin on the Proliferation of Melanoma B16 Cells in vitro

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