固碳微生物菌株的分离鉴定及其固碳能力测定

doi:10.13560/j.cnki.biotech.bull.1985.2018-0544

生物技术通报 ›› 2019, Vol. 35 ›› Issue (1): 90-97.doi: 10.13560/j.cnki.biotech.bull.1985.2018-0544

固碳微生物菌株的分离鉴定及其固碳能力测定

郭珺¹, 樊芳芳², 王立革¹, 武爱莲¹, 郑军³

1. 山西省农业科学院农业环境与资源研究所山西省土壤环境与养分资源重点实验室,太原 030031;
2. 山西省农业科学院高粱研究所,晋中 030600;
3. 山西省农业科学院小麦研究所,临汾 041000

收稿日期:2018-06-12 出版日期:2019-01-26 发布日期:2019-01-23
作者简介:郭珺,女,硕士,副研究员,研究方向：农业环境微生物资源利用;E-mail：guojun1950@163.com
基金资助:
国家重点研发计划子课题（2017YFD0800405）,山西省煤基重点科技攻关项目（FT201402-15）,山西省重点研发计划项目（201703D221008-2）

Isolation,Identification of Carbon-fixing Bacteria and Determination of Their Carbon-fixing Abilities

GUO Jun¹, FAN Fang-fang², WANG Li-ge¹, WU Ai-Lian¹, ZHENG Jun³

1. Institute of Agricultural Environment & Resource,Shanxi Academy of Agricultural Sciences,Key Laboratory of Soil Environment & Nutrient Resources of Shanxi Province,Taiyuan 030031;
2. Sorghum Institute of Shanxi Academy of Agricultural Sciences,Jinzhong 030600;
3. Wheat Research Institute, Shanxi Academy of Agricultural Sciences,Linfen 041000

Received:2018-06-12 Published:2019-01-26 Online:2019-01-23

摘要/Abstract

摘要： 利用微生物回收和固定CO₂气体的生物固碳方法,成为解决“温室效应”这一重大环境问题的焦点,本研究目的是分离筛选固碳微生物菌株。利用无碳源无机培养基从活性污泥、沼液和设施土壤中分离筛选出以CO₂为碳源的菌株24株,选取其中生长较快的8株菌进行cbbL基因、形态观察、生理生化反应测定和16S rDNA测序分析,将测序结果在BlAST数据库中比对,进行固碳菌株的分子鉴定,并对其菌体含量和RubisCO酶活性进行比较。选取RubisCO酶活性最高的菌株C2-8R,进行土壤施用试验,通过土壤RubisCO酶活性的测定,确定分离筛选的固碳菌的固碳能力。研究表明,可通过无碳源培养基进行固碳微生物菌株的筛选,筛选的固碳菌分别隶属于假单胞菌属和嗜甲基菌属,并可通过RubisCO 酶活性来反映微生物的固碳能力。

关键词: 固碳微生物菌株, cbbL基因, 16S rDNA, RubisCO酶活性

Abstract: Currently,as a biological carbon sequestration strategy,microbial recovery and fixing of CO₂ gas,has become the focus of interest in dealing with the major environmental problem of greenhouse effect. The purpose of this study is to separate and screen efficient carbon-fixing microbial strains. By using the carbon-free source inorganic medium,24 kinds of strains with CO₂ as carbon source were separated from facilities soil,activated sludge and biogas slurry. Then,the 8 strains of bacteria that grew faster were selected to conduct their morphological observations,physiological and biochemical responses as well as to perform cbbL gene and 16S rDNA sequencing analysis. Further,the sequencing results were compared with the data in BlAST database and the molecular characterization of the 8 carbon-fixing strains was carried out along with the detection of the strain content and the RubisCO enzyme activity. Finally,the C2-8R with the highest RubisCO activity among the 8 strains was selected for soil application test,and the carbon sequestration capacity of the C2-8R was determined through the detection of the RubisCO enzyme activity in soil. The above results revealed that carbon sequestration strains can be screened through carbon-free medium. The screened carbon-fixing bacteria are subordinate to the strains of Pseudomonas sp. and Methanotrophs sp.,and can be used to indicate the microbial carbon-fixing ability through RubisCO enzyme activity.

Key words: carbon-fixing microbial strains, cbbL gene, 16S rDNA, RubisCO activity

郭珺, 樊芳芳, 王立革, 武爱莲, 郑军. 固碳微生物菌株的分离鉴定及其固碳能力测定[J]. 生物技术通报, 2019, 35(1): 90-97.

GUO Jun, FAN Fang-fang, WANG Li-ge, WU Ai-Lian, ZHENG Jun. Isolation,Identification of Carbon-fixing Bacteria and Determination of Their Carbon-fixing Abilities[J]. Biotechnology Bulletin, 2019, 35(1): 90-97.

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固碳微生物菌株的分离鉴定及其固碳能力测定

Isolation,Identification of Carbon-fixing Bacteria and Determination of Their Carbon-fixing Abilities

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