一株α-苯乙胺的微生物降解菌的优选及其最佳生长条件研究

doi:10.13560/j.cnki.biotech.bull.1985.2019-0858

摘要/Abstract

摘要：

针对磷霉素制药废水处理难的问题,采用生物强化处理技术,在前期获得两株磷霉素制药废水中α-苯乙胺降解菌P1和P2的基础上,优选出一株α-苯乙胺优势降解菌,并对其最佳生长条件进行研究。结果表明：两株菌P1和P2中优势降解菌为P2;其最佳生长条件为：接种量20%,温度35℃,pH 7;P2的降解效率受传代次数的影响,传代17代以后P2开始出现缓慢的退化现象;将α-苯乙胺优势降解菌投至实际磷霉素制药废水中,α-苯乙胺降解效率为76%。

关键词: 磷霉素, 制药废水, α-苯乙胺, 优势降解菌, 生长条件

Abstract:

In order to solve the difficult problem of treating fosfomycin pharmaceutical wastewater,a dominant strain was selected by bioenhanced treatment based on 2 α-phenylethylamine degrading bacteria P1 and P2 in fosfomycin pharmaceutical wastewater from previous work,and the optimal growth conditions were studied. The results showed that the dominant degrading one was P2 among P1 and P2. The optimal growth conditions were as follows：inoculation volume 20%,温度35℃,pH 7. The degradation efficiency of P2 was affected by the number of passage. After 17 generations,P2 began to degrade slowly. The degradation efficiency of α-phenylethylamine was 76% when the α-phenylethylamine dominant bacterium was put into the actual fosfomycin pharmaceutical wastewater.

Key words: fosfomycin, pharmaceutical wastewater, α-phenethylamine, dominant bacteria, growth condition

丁丽, 曾萍, 成璐瑶, 宋永会, 胡丽娜. 一株α-苯乙胺的微生物降解菌的优选及其最佳生长条件研究[J]. 生物技术通报, 2021, 37(3): 65-74.

DING Li, ZENG Ping, CHENG Lu-yao, SONG Yong-hui, Hu Li-na. Optimization of a Strain of Microbe Degrading α-phenylethylamine and Its Optimal Growth Conditions[J]. Biotechnology Bulletin, 2021, 37(3): 65-74.

图/表 17

图1 苯乙胺结构式

图2 菌株16S rDNA PCR产物琼脂糖凝胶电泳图 M：分子量标准;P1、P2：基因片段

图3 菌株进化树

图4 菌种生长曲线

图5 不同接种量下纯菌的生长量变化 A：10%接种量;B：20%接种量;C：30%接种量

图6 不同接种量下α-苯乙胺浓度变化 A：10%接种量;B：20%接种量;C：30%接种量

图7 不同接种量下α-苯乙胺的降解效率

图8 菌株P2在20%接种量下的生长量与α-苯乙胺浓度变化

表1 菌株P1、P2对不同基质的耐受状况

生长基质	P1生长状况	P2生长状况
磷霉素钠四环素	— —	+ +
3,5-二氯苯酚	+	+
邻苯二甲酸	+	+

图9 不同温度下P2的生长量变化

图10 不同温度下P2对α-苯乙胺的降解

图11 不同pH下P2的生长量变化

图12 不同pH下P2对α-苯乙胺的降解

图13 不同传代次数下P2在对数增长期的生长变化

图14 不同传代次数下α-苯乙胺的浓度变化

图15 菌株P2在实际磷霉素制药废水中生长量

图16 投加P2后实际磷霉素制药废水中α-苯乙胺浓度

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