一株华北油田地层水细菌HB-1的分离及多相分类鉴定

生物技术通报 ›› 2013, Vol. 0 ›› Issue (6): 110-115.

一株华北油田地层水细菌HB-1的分离及多相分类鉴定

李青¹, 刘洋², 吴刚¹, 杜慧竟², 游靖¹, 李红², 柯从玉¹, 张欣², 余吉良¹, 赵婷²

(1.华北油田采油工程研究院, 任丘 062552;2.中国食品发酵工业研究院中国工业微生物菌种保藏管理中心, 北京 100027)

收稿日期:2013-06-20 修回日期:2013-06-20 出版日期:2013-06-20 发布日期:2013-06-20
基金资助:
中国石油天然气股份有限公司华北油田分公司重大科技项目(2012-HB-z231), 中国食品发酵工业研究院科技发展基金(博士基金)项目(2012KJFZ-BS-01)

Identification on a Bacterial Strain HB-1 Separated from Stratum Water in Huabei Oilfield

Li Qing¹ Liu Yang² Wu Gang¹ Du Huijing² You Jing¹ Li Hong² Ke Congyu¹ Zhang Xin² Yu Jiliang¹ Zhao Ting²

(1. Petroleum Production Engineering Research Institute of Huabei Oilfield Company, Renqiu 062552;2. China Center of Industrial Culture Collection, China National Research Institute of Food and Fermentation Industries, Beijing 100027)

Received:2013-06-20 Revised:2013-06-20 Published:2013-06-20 Online:2013-06-20

摘要/Abstract

摘要： 自华北油田地层水中分离得到的细菌HB-1, 对其进行形态学、生理生化特征鉴定、化学成分分析以及遗传特征测定, 并结合16S rRNA基因序列分子生物学鉴定及系统发育学分析。结果表明, 该菌株归属于螯合球菌属(Chelatococcus sp.) , 并且可鉴定为大邱螯合球菌(Chelatococcus daeguensis) 。其最适生长温度为37℃, 主要极性脂为DPG、PE、PG及PC, 醌为Q-10, 主要脂肪酸组成分别为C_{18: 1} ω7c(39.49%) 和C_{19: 0} cyclo ω8c(30.39%) , HB-1具有氧化酶、接触酶、酯酶(C4) 、类脂酯酶(C8) 、白氨酸芳胺酶、缬氨酸芳胺酶、胱氨酸芳胺酶、胰蛋白酶、酸性磷酸酶及萘酚-AS-BI-磷酸水解酶等酶活性。该菌株是首次从油田地层水中分离并鉴定得到的螯合球菌。

关键词: 华北油田, 地层水, 大邱螯合球菌, HB-1, 多相分类鉴定

Abstract: A bacterial strain HB-1 was isolated from stratum water in Huabei Oilfield, and identificated by polyphasic taxonomy basing on morphologic and physiologic methods, chemical composition analysis, genetic characteristics test and 16S rRNA gene phylogenetic analysis. The result showed that the strain was a member of the genus Chelatococcus, and was belong to the known species of Chelatococcus daeguensis. 37℃ was its optimum growth temperature. The major polar lipids presented in strain HB-1 were DPG, PE, PG and PC. Major fatty acids were C_{18: 0} ω7c(39.49%)and C_{19: 0} cyclo ω8c(30.39%), and ubiquinone Q-10 was the predominant quinone. HB-1 had enzyme activity of oxidase, catalase, esterase(C4), esterase lipase(C8), leucine arylamidase, valine arylamidase, cystine arylamidase, trypsin, acid phosphatase and naphthol-AS-BI-phosphate hydrolase. Chelatococcus daeguensis HB-1 was isolated from stratum water in Huabei Oilfield for the first time.

Key words: Huabei Oilfield, Stratum water, Chelatococcus daeguensis, HB-1, Polyphasic taxonomy

李青, 刘洋, 吴刚, 杜慧竟, 游靖, 李红, 柯从玉, 张欣, 余吉良, 赵婷. 一株华北油田地层水细菌HB-1的分离及多相分类鉴定[J]. 生物技术通报, 2013, 0(6): 110-115.

Li Qing, Liu Yang, Wu Gang, Du Huijing, You Jing, Li Hong, Ke Congyu, Zhang Xin, Yu Jiliang, Zhao Ting. Identification on a Bacterial Strain HB-1 Separated from Stratum Water in Huabei Oilfield[J]. Biotechnology Bulletin, 2013, 0(6): 110-115.

参考文献

[1] 邓丽萍, 蒋新兴, 焦龙进.微生物采油菌种性能评价的方法研究[J].内蒙古石油化工, 2012, 12:9-10.
[2] Yoon JH, Kang SJ, Im WT, et al. Chelatococcus daeguensis sp. nov., isolated from waste water of a textile dye works, and emended description of the genus Chelatococcus[J]. IJSEM, 2008, 58(9):2224-2228.
[3] 刘洋, 赵婷, 姚粟, 等.一株芝麻香型白酒高温大曲嗜热放线菌的分离与鉴定[J].生物技术通报, 2012(10):210-216.
[4] Liu Y, Lin L, Qiu FB, et al. Paenibacillus hunanensis sp. nov., isolated from rice seeds[J]. IJSEM, 2010, 60(6):1266-1270.
[5] Chun J, Lee JH, Jung Y, et al. EzTaxon:a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences[J]. IJSEM, 2007, 57(10):2259-2261.
[6] Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W:improving the sensitivity of progressive multiple sequence alignment through sequ-ence weighting, position-specific gap penalties and weight matrix choice[J]. Nucleic Acids Res, 1994, 22(22):4673-4680.
[7] Tamura K, Dudley J, Nei M, et al. MEGA4:molecular evolutionary genetics analysis(MEGA)software version 4.0[J]. Molecular Biology and Evolution, 2007, 24(8):1596-1599.
[8] 东秀珠, 蔡妙英.常见细菌系统鉴定手册[M].北京:科学出版社, 2001:399-402.
[9] Auling G, Busse HJ, Egli T, et al. Description of the Gram-negative, obligately aerobic, nitrilotriacetate(NTA)-utilizing bacteria as Chelatobacter heintzii, gen. nov., sp. nov., and Chelatococcus asacch-arovorans, gen. nov., sp. Nov[J]. Syst Appl Microbiol, 1993, 16:104-112.
[10] Panday D, Das SK. Chelatococcus sambhunathii sp. nov., a modera-tely thermophilic alphaproteobacterium isolated from hot spring sediment[J]. IJSEM, 2010, 60(4):861-865.
[11] 张苗, 黄少斌.高温好氧反硝化菌的分离鉴定及其反硝化性能研究[J].环境科学, 2011, 32(1):259-265.