滑桃树种子提取物对其内生菌活性化合物的影响

生物技术通报 ›› 2013, Vol. 0 ›› Issue (10): 93-97.

滑桃树种子提取物对其内生菌活性化合物的影响

吴欣

（国家海洋局第三海洋研究所，厦门 361005）

收稿日期:2013-03-19 修回日期:2013-10-15 出版日期:2013-10-14 发布日期:2013-10-15
作者简介:吴欣，女，博士，工程师，研究方向：微生物学；E-mail ：careless78@gmail.com

Influence of Trewia nudiflora Seed Extract on the Antifungal Compound of Endophyte

Wu Xin

（Third Institute of Oceanography State Oceanic Administration，Xiamen 361005）

Received:2013-03-19 Revised:2013-10-15 Published:2013-10-14 Online:2013-10-15

摘要/Abstract

摘要：

Streptomyces sp. WXC 菌株是从滑桃树（Trewia nudiflora）种子中分离到的一株内生菌，滑桃树种子提取物对Streptomyces sp. WXC 菌株的生长和活性化合物的表达有促进作用。运用HPLC 、RT-PCR 和Real-time PCR 技术，研究Streptomyces sp. WXC 菌株在加入滑桃树种子提取物和不加入滑桃树种子提取物的两种条件下，其活性化合物富伦菌素B 产量及其生物合成基因表达量的差异。结果显示，滑桃树种子提取物诱导了活性化合物富伦菌素B 生物合成基因的表达，从而提高了富伦菌素B 的产量。首次揭示了植物与其内生放线菌之间的化学作用。

关键词: 滑桃树种子提取物, Streptomyces sp., WXC 菌株, 富伦菌素B, 生物合成基因

Abstract:

The chemical interactions between endophytes and plant hosts have been an interesting topic since the coining of the concept of symbiosis and co-evolution. Streptomyces sp. WXC was an endophytic strain isolated from the seed of Trewia nudiflora. Culture medium optimization revealed that supplemented with the aqueous extract of T. nudiflora seeds, S. sp. WXC grew faster, and the production of frenolicin B was increased by ca. 3-fold as well. Real-time PCR analysis revealed that the expression levels of frenolicin B biosynthetic genes were induced by the seed extract, which correlated to the increased production of frenolicin B indicated by HPLC analysis. These results indicated that host’s chemical components may induce the endophytic S. sp. WXC to increase the production of antifungal compound. This work shows, for the first time, that intimate chemical interaction exists between endophytic actinomycete and its host.

Key words: T. nudiflora seed extract, Streptomyces sp. WXC, Frenolicin B, Biosynthetic genes

吴欣. 滑桃树种子提取物对其内生菌活性化合物的影响[J]. 生物技术通报, 2013, 0(10): 93-97.

Wu Xin . Influence of Trewia nudiflora Seed Extract on the Antifungal Compound of Endophyte[J]. Biotechnology Bulletin, 2013, 0(10): 93-97.

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