一株耐盐弧菌Vibrio sp.K1-L的分离与鉴定

生物技术通报 ›› 2014, Vol. 0 ›› Issue (4): 127-131.

一株耐盐弧菌Vibrio sp.K1-L的分离与鉴定

王菲, 刘抗, 范家同, 成守亮, 王子元

(江苏师范大学生命科学学院，徐州 221116)

收稿日期:2013-10-30 出版日期:2014-04-29 发布日期:2014-04-29
作者简介:王菲，女，硕士研究生，研究方向：生物化学与分子生物学；E-mail：xzwangfei@126.com
基金资助:
国家自然科学基金项目(30571071)，江苏师范大学王子元教授科研启动基金(KY2009101)，江苏高校优势学科建设工程项目，江苏省教育厅优秀创新团队项目

Isolation and Identification of a Halotolerant—Vibrio sp. K1-L

Wang Fei, Liu Kang, Fan Jiatong, Cheng Shouliang, Wang Ziyuan

(School of Life Science，Jiangsu Normal University，Xuzhou 221116)

Received:2013-10-30 Published:2014-04-29 Online:2014-04-29

摘要/Abstract

摘要： 从山西运城盐湖水样中分离出一株细菌K1-L，能在2%-10%的MGM固体培养基上出现群游现象。该菌株为革兰氏阴性菌，周生鞭毛，电镜下大小约为0.4 μm×(1-2)μm，其菌落形态为圆形，灰色。菌株K1-L的最适生长盐浓度、温度和pH值的范围分别为2%-6%、30-40℃和6.0-7.0，GC含量为47.06%。通过对其形态学、生理生化试验，16S rRNA基因序列及系统发育学的分析，发现其与Vibrio diabolicus HE800T和Vibrio rotiferianus LMG 21460T等细菌的16S rRNA有高度同源性，最大的相似性均为99％，确定该菌株K1-L为弧菌属(Vibrio)。

关键词: 运城盐湖, 弧菌属, 耐盐菌, 系统发育分析

Abstract: Strain K1-L, isolated from Yuncheng Salt Lake, Shanxi Province, could swim on the 2%-10% MGM medium. The strain K1-L was Gram-negative with polar flagellum and was 0.4 μm ×(1-2)μm under the scanning electron microscope. Strain K1-L appeared grey, rounded colony. Its optimum salt concentration, temperature and pH ranges were 2%-6%, 30-40℃ and 6.0-8.0. The GC content is 47.06%. After comparing the traditional characteristics of morphology, physiological biochemistry and 16S rRNA sequence analysis, we discovered that the strain K1-L was highly homologous to the Vibrio diabolicus HE800^T and Vibrio rotiferianus LMG 21460^T. The maximum similarity was 99%. It was asserted that K1-L was belonged to the genus Vibrio, we proposed the name Vibrio sp. K1-L for this strain.

Key words: Yuncheng salt lake, Vibrio, Halotolerant, Phylogenetic analysis

王菲, 刘抗, 范家同, 成守亮, 王子元. 一株耐盐弧菌Vibrio sp.K1-L的分离与鉴定[J]. 生物技术通报, 2014, 0(4): 127-131.

Wang Fei, Liu Kang, Fan Jiatong, Cheng Shouliang, Wang Ziyuan. Isolation and Identification of a Halotolerant—Vibrio sp. K1-L[J]. Biotechnology Bulletin, 2014, 0(4): 127-131.

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