斑玉蕈不同交配型的原生质体单核菌株差异分析

生物技术通报 ›› 2014, Vol. 0 ›› Issue (9): 131-135.

斑玉蕈不同交配型的原生质体单核菌株差异分析

潘越^1,2,陈辉²,冯志勇^1,2,赵静³,

1.南京农业大学生命科学学院，南京 210095;
2.国家食用菌工程技术研究中心农业部南方食用菌资源利用重点开放实验室上海市农业遗传育种重点开发实验室上海市农业科学院食用菌研究所，上海 201403;
3.上海光明森源生物科技有限公司，上海 201408

收稿日期:2014-02-12 出版日期:2014-09-15 发布日期:2014-09-07
作者简介:潘越，女，硕士研究生，研究方向：食用菌遗传育种；E-mail：skyside.Good@163.com
基金资助:
上海科委重点科技攻关项目（11391901003）

Variance Analysis of Different Mating Type Strains in Mononuclear Protoplast of Hypsizygus marmoreus

Pan Yue^1,2,Chen Hui²,Feng Zhiyong^1,2,Zhao Jing³

1. College of Life Sciences，Nanjing Agricultural University，Nanjing 210095;
2. National Research Center for Edible Fungi Biotechnology and Engineering，Shanghai Key Laboratory of Agricultural Genetics and Breeding， Institute of Edible Fungi，Shanghai Academy of Agricultural Sciences，Shanghai 201403;
3. Shanghai Bright Esunyes Bio-tech Co. Lyd，Shanghai 201408

Received:2014-02-12 Published:2014-09-15 Online:2014-09-07

摘要/Abstract

摘要： 对2个斑玉蕈菌株进行了原生质体分离与交配型鉴定，并对不同交配型单核体的代谢特性进行了研究。结果表明，2个斑玉蕈菌株均出现2种交配型，2种交配型比例不一致，且2个菌株的两种单核体的分离比例均不符合1∶1分离比，交配型比例上出现明显的偏差现象。2个菌株中，不同交配型单核菌株的菌丝形态有明显差别，代谢过程中的菌丝生物量及发酵液的pH、还原糖含量和电导率均存在差异，表明在这2个菌株中不同交配型单核菌株的菌丝形态及代谢特性与交配型因子相关。

关键词: 斑玉蕈, 单核原生质体, 交配型, 菌丝形态, 代谢特性

Abstract: Tow Hypsizygus marmoreus strains were studied by protoplast isolation and mating-type identification. Metabolic characteristics of different mating type strains were studied. Results showed that each of the 2 Hypsizygus marmoreus strains had 2 mating type. The ratio of two mating-type monokaryons of 2 strains was not 1∶1 and the proportion of mating types had different phenomenon. Different kinds of mating type had different hyphae form and there was difference between the conductivity, the pH, the content of reducing sugar of the broth and the mycelial biomass in different mating type strains, which indicated that hyphae form and metabolic characteristic had relations with mating factors in this two strains.

Key words: Hypsizygus marmoreus , Monokaryon protoplast, Mating type Hyphae form, Metabolic characteristic

潘越,陈辉,冯志勇,赵静,. 斑玉蕈不同交配型的原生质体单核菌株差异分析[J]. 生物技术通报, 2014, 0(9): 131-135.

Pan Yue,Chen Hui,Feng Zhiyong,Zhao Jing. Variance Analysis of Different Mating Type Strains in Mononuclear Protoplast of Hypsizygus marmoreus[J]. Biotechnology Bulletin, 2014, 0(9): 131-135.

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