生物技术通报 ›› 2019, Vol. 35 ›› Issue (2): 64-72.doi: 10.13560/j.cnki.biotech.bull.1985.2018-0803

• 研究报告 • 上一篇    下一篇

抗镉内生细菌阿耶波多氏芽孢杆菌的分离鉴定及生物学特性

刘军生, 解修超, 罗阳兰, 邓百万, 柏秋月, 燕孟琛, 白星   

  1. 1. 陕西理工大学 陕西省食药用菌工程技术研究中心,汉中 723001;
    2. 陕西理工大学 生物科学与工程学院,汉中723001
  • 收稿日期:2018-09-13 出版日期:2019-02-26 发布日期:2019-03-07
  • 作者简介:刘军生,男,硕士研究生,研究方向:微生物保育与开发;E-mail:18763899522@163.com
  • 基金资助:
    秦巴山区生物资源综合开发协同创新中心自然科学研究项目(QBXT-17-12),陕西省教育厅科研专项项目(16JK1158)

Isolation,Identification,and Biological Characteristics of a Cadmium-Resistant Endophytic Bacteria Bacillus aryabhattai

LIU Jun-sheng, XIE Xiu-chao, LUO Yang-lan, DENG Bai-wan, BAI Qiu-yue, YAN Meng-chen, BAI Xing   

  1. 1. Shaanxi Provincial Engineering Research Center of Edible & Medicinal Fungi,Shaanxi University of Technology,Hanzhong 723001;
    2. School of Biological Science & Engineering,Shaanxi University of Technology,Hanzhong 723001
  • Received:2018-09-13 Published:2019-02-26 Online:2019-03-07

摘要: 对分离得到的一株可在Cd2+浓度为900 mg/L 培养基中生长的抗镉内生细菌GR42进行鉴定并研究其生物学特性,为其应用提供理论依据。利用16S rDNA序列分析、形态学和生理生化实验鉴定其种属,探究培养条件对菌株生长的影响并研究其镉去除率、铅锌耐受性、促生特性以及对小麦幼苗抗镉能力的促进作用。经鉴定该菌株为阿耶波多氏芽孢杆菌(Bacillus aryabhattai),最适温度、pH、装液量和盐浓度分别为28℃、7.0、50 mL/250 mL和5-10 g/L。Cd2+浓度为800 mg/L 时,镉去除率可达41.1%,可耐受2 000 mg/LPb2+、1 200 mg/L Zn2+,铁载体活性76.74%,ACC脱氨酶活性0.627 U/mg,溶磷量为13.801 mg/g,解钾量为8.868 mg/g,并对小麦幼苗的抗镉能力有显著促进作用。抗镉内生细菌GR42为阿耶波多氏芽孢杆菌,铅锌镉抗性和镉去除率较高,并有一定的促生能力,在植物促生和Cd污染土壤修复方面具有一定的应用潜力。

关键词: 阿耶波多氏芽孢杆菌, 抗镉内生细菌, 促生能力, 土壤修复

Abstract: A cadmium-resistant endophytic bacterium GR42,which may grow in the culture medium with 900 mg/L of Cd2+ concentration,was identified and studied for its biological characteristics in order to provide theoretical basis for its application. 16S rDNA sequence analysis,morphological,as well as physiological and biochemical experiments were used to identify the species,to explore the influences of culture conditions on the growth of the strain,and to test its cadmium-removed rate,its tolerance to lead and zinc,growth promoting characteristics,and the promotion effect on the cadmium-resistance of wheat seeding. As results,the strain GR42 was identified as Bacillus aryabhattai,and the optimal temperature,pH,loading volume,and salt concentration was 28℃,7.0,50 mL/250 mL,and 5-10 g/L,respectively. Cadmium-removed rate was 41.1% at 800 mg/L Cd2+concentration;toleration of Pb2+ and Zn2+ was 2000 mg/L and 1200 mg/L respectively;siderophore activity was 76.74%,and ACC deaminase activity was 0.627 U/mg;the capacity of the strain dissolving phosphorus and potassium was 13.801 mg/g and 8.868 mg/g,respectively;it also showed significant promoting effects on the cadmium-resistance of wheat seedlings. In conclusion,the cadmium-resistant endophytic bacterium GR42 is B. aryabhattai,its lead-zinc-cadmium resistance and cadmium-removed rate are high,and it has ability of promoting growth,therefore it owns application potential in promoting plant growth and remediating cadmium-contamination soil.

Key words: Bacillus aryabhattai, cadmium-resistant endophytic bacteria, ability of promoting growth, soil remediation