凝结芽孢杆菌次级代谢挖掘与泛基因组分析

doi:10.13560/j.cnki.biotech.bull.1985.2020-0097

摘要/Abstract

摘要：

为进一步加深对凝结芽孢杆菌基因组的了解,同时探索其次级代谢通路。从NCBI数据库中获得33个凝结芽孢杆菌的基因组,利用泛基因组分析软件和次级代谢产物挖掘软件对其进行分析。得到33株凝结芽孢杆菌的基因组大小在2.059 47-3.694 84 Mbp之间,GC含量范围为46.2%-47.5%。对11株有完整基因组的凝结芽孢杆菌进行泛基因组分析,发现其泛基因组含有5 899个基因家族,核心基因组由2 152个基因家族组成,并鉴定出2 255个特有基因。通过次级代谢产物合成基因簇分析,33个凝结芽孢杆菌基因组中发现有8类、79个次级代谢基因簇,主要的合成基因簇是细菌素、内酯和糖类。通过本研究预测出凝结芽孢杆菌的泛基因组和核心基因组大小及其次级代谢产物,有助于了解凝结芽孢杆菌,更好地利用该菌株提供相关信息。

关键词: 凝结芽孢杆菌, 泛基因组, 次级代谢基因簇

Abstract:

This study was to further understand the genome of Bacillus coagulans and to explore its secondary metabolic pathways. The genomes of 33 B. coagulans were downloaded from the NCBI database and analyzed by pan-genomic analysis software and secondary metabolite mining software. The genome size of 33 strains of B. coagulans was between 2.059 47-3.694 84 Mbp and the GC content ranged from 46.2% to 47.5%. Pan-genome analysis of 11 strains of B. coagulans with complete genome level revealed that the pan-genome contained 5 899 cluster genes,the core genome consisted of 2 152 cluster genes,and 2 255 strain-specific genes were identified. Based on the analysis of secondary metabolite synthesis gene clusters,79 secondary metabolite gene clusters of 8 groups were found in the 33 B. coagulans genomes. The major synthetic gene clusters were bacteriocins,lactones and sugars. In brief,the pan-genome and core genome sizes of B. coagulans and their secondary metabolites through can be predicted in this study,which is conducive to understanding B. coagulans and providing relevant information further better utilization of this strain.

Key words: Bacillus coagulans, pan-genome, secondary metabolic gene cluster

钱文江, 汪步青, 李葳茜, 杨雪苗, 刘洪伟, 张丽萍. 凝结芽孢杆菌次级代谢挖掘与泛基因组分析[J]. 生物技术通报, 2020, 36(10): 88-98.

QIAN Wen-jiang, WANG Bu-qing, LI Wei-xi, YANG Xue-miao, LIU Hong-wei, ZHANG Li-ping. Secondary Metabolic Pathway Mining and Pan-genome Analysis of Bacillus coagulans[J]. Biotechnology Bulletin, 2020, 36(10): 88-98.

图/表 9

参考文献 29

[1]	郑兆娟, 林茜, 臧颖, 等. 氮源和无机盐对2株凝结芽孢杆菌L-乳酸发酵影响的研究[J]. 生物学杂志, 2015,32(6):55-59.
	Zheng ZJ, Lin Q, Zang Y, et al. Effects of nitrogen source and inorganic salt on the L-lactic acid production of two Bacillus coagulans strains[J]. Journal of Biology, 2015,32(6):55-59.
[2]	李刚, 傅玲琳, 王彦波. 益生菌凝结芽孢杆菌胞外产物抑菌特性研究[J]. 食品科技, 2013,38(10):20-24.
	Li G, Fu LL, Wang YB. Antimicrobial activities of extracellular products produced by the probiotic Bacillus coagulans[J]. Food Science and Technology, 2013,38(10):20-24.
[3]	赵钰, 傅玲琳, 王彦波, 等. 凝结芽孢杆菌抑菌物质的理化特性研究[J]. 食品研究与开发, 2015,36(1):20-25.
	Zhao J, Fu LL, Wang YB, et al. Physiochemical properties of antibacterial substance produced by Bacillus coagulans LL1103[J]. Food Research and Development, 2015,36(1):20-25.
[4]	Hyronimus B, Le MC, Urdaci MC. Coagulin, a bacteriocin-like inhibitory substance produced by Bacillus coagulans I₄[J]. Journal of Applied Microbiology, 1998,85(1):42-50. URL pmid: 9721655
[5]	Huszcza E, Burczyk B. Surfactin isoforms from Bacillus coagulans[J]. Zeitschrift für Naturforschung C, 2006,61(9-10):727-733.
[6]	Kodali VP, Das S, Sen R. An exopolysaccharide from a probiotic:Biosynjournal dynamics, composition and emulsifying activity[J]. Food Research International, 2009,42(5-6):695-699.
[7]	赵钰. 凝结芽孢杆菌抑菌物质分离鉴定及其对大黄鱼保鲜效果的研究[D]. 杭州:浙江工商大学, 2015.
	Zhao J. Isolation and identification of antibacterial substance prouduced by Bacillus coagulans and apply it in large yellow croaker preservation[J]. Hangzhou:Zhejiang Gongshang University, 2015.
[8]	刘全永, 崔保伟, 胡江春, 等. 海洋细菌Bacillus coagulans spp. heishijiaosis发酵液抗真菌活性及其机制[J]. 中国海洋药物, 2015,34(3):65-70.
	Liu YQ, Cui BW, Hu JC, et al. Antifungal activity and mechanism of the bioactive substance derived from marine bacteria Bacillus coagulans spp. heishijiaosis fermentation broth[J]. Chinese Journal of Marine Drugs, 2015,34(3):65-70.
[9]	庄绪冉, 朱泳璋. 细菌泛基因组学的研究[J]. 上海交通大学学报:医学版, 2012,32(11):1440-1443.
	Zhuang XR, Zhu YZ. Research progress of bacterial pan-genome[J]. Journal of Shanghai Jiaotong University:Medical Science, 2012,32(11):1440-1443.
[10]	赵永兵. 泛基因组学分析方法开发及应用[D]. 北京:中国科学院, 2014.
	Zhao YB. Development and application of pan-genomics analytical method[J]. Beijing:Chinese Academy of Sciences, 2014.
[11]	Vernikos G, Medini D, Riley DR, et al. Ten years of pan-genome analyses[J]. Current Opinion in Microbiology, 2014,23:148-154. URL pmid: 25483351
[12]	Kim Y, Koh IS, Young LM, et al. Pan-genome analysis of Bacillus for microbiome profiling[J]. Scientific Reports, 2017,7(1):10984. URL pmid: 28887527
[13]	付静, 秦启伟. 30株大肠杆菌的泛基因组学特征分析[J]. 遗传, 2012,34(6):765-772.
	Fu J, Qin QW. Pan-genomics analysis of 30 Escherichia coli genomes[J]. Hereditas, 2012. 34(6):765-772.
[14]	杨晓雯. 布鲁氏菌等三种兼性胞内寄生菌比较基因组分析及应用[D]. 北京:中国农业大学, 2018.
	Yang XW. Comparative genomics analysis and amplication of three facultative intracellular pathogens such as Brucella spp.[J]. Beijing:China Agricultural University, 2018.
[15]	Xing J, Li X, Sun Y, et al. Comparative genomic and functional analysis of Akkermansia muciniphila and closely related species[J]. Genes & Genomics, 2019,41(4):1253-1264.
[16]	Lassaad B, Ali CB, Imen R, et al. Comparative genomics of Bacillus amyloliquefaciens strains reveals a core genome with traits for habitat adaptation and a secondary metabolites rich accessory genome[J]. Frontiers in Microbiology, 2017,8:1438. doi: 10.3389/fmicb.2017.01438 URL pmid: 28824571
[17]	宋宇琴. 德氏乳杆菌保加利亚亚种的群体遗传学和功能基因组学研究[D]. 呼和浩特:内蒙古农业大学, 2018.
	Song YQ. Study on population genetics and functional genomics of Lactobacillus delbrueckii subsp. bulgaricus[D]. Hohhot:Inner Mongolia Agricultural University, 2018.
[18]	Cho JC, Tiedje JM. Bacterial species determination from DNA-DNA hybridization by using genome fragments and DNA microarrays[J]. Applied & Environmental Microbiology, 2001,67(8):3677-3682. URL pmid: 11472947
[19]	Frederic B, Silander OK, Mikhail P, et al. Automated reconstruction of whole-genome phylogenies from short-sequence reads[J]. Molecular Biology and Evolution, 2014,31(5):1077-1088. doi: 10.1093/molbev/msu088 URL pmid: 24600054
[20]	王美美, 张建中, 李凤琴. 基于内转录间隔区和β-微管蛋白部分基因及相关序列扩增多态性分子标记技术推断红曲霉系统发育关系[J]. 中国食品卫生杂志, 2018,30(6):563-569.
	Wang MM, Zhang JZ, Li FQ. Genetic diversity analysis of Monascus strains using internal transcribed space, partial β-tubulin gene and sequence-related amplification polymorphism markers[J]. Chinese Journal of Food Hygiene, 2018,30(6):563-569.
[21]	Yongbing Z, Chen S, Dongyu Z, et al. PGAP-X:extension on pan-genome analysis pipeline[J]. BMC Genomics, 2018,19(S1):36.
[22]	Ozer EA, Allen JP, Hauser AR. Characterization of the core and accessory genomes of Pseudomonas aeruginosa using bioinformatic tools Spine and AGEnt[J]. BMC Genomics, 2014,15:737. URL pmid: 25168460
[23]	Blin K, Simon S, Katharina S, et al. antiSMASH 5. 0:updates to the secondary metabolite genome mining pipeline[J]. Nucleic Acids Research, 2019,47(W1):W81-W87.
[24]	靳泽星, 秦娅, 王洁丽, 等. 芽孢杆菌SK007的分类鉴定及其拮抗植物病原菌的功能分析[J]. 微生物学通报, 2019,46(10):2591-2600.
	Jin ZX, Qin Y, Wang JL, et al. Classification and identification of Bacillus sp. SK007 with biocontrol activity[J]. Microbiology China, 2019,46(10):2591-2600.
[25]	孙晨. 比较基因组学信息可视化系统(CGVS系统)开发与应用[D]. 北京:中国科学院大学, 2013.
	Sun C. Research and development of comparative genomics visualization system(CGVS)[D]. Beijing:The University of Chinese Academy of Sciences, 2013.
[26]	Blin K, Medema MH, Kottmann R, et al. The antiSMASH database, a comprehensive database of microbial secondary metabolite biosynthetic gene clusters[J]. Nucleic Acids Research, 2016,45:D555-D559. URL pmid: 27924032
[27]	Riazi S, Wirawan RE, Badmaev V, et al. Characterization of lactosporin, a novel antimicrobial protein produced by Bacillus coagulans ATCC 7050[J]. Journal of Applied Microbiology, 2009,106(4):1370-1377. doi: 10.1111/j.1365-2672.2008.04105.x URL pmid: 19191946
[28]	孙天拥. 细菌界次级代谢产物生物合成基因簇的基因组学注释和比较研究[D]. 济南:山东大学, 2016.
	Sun TY. Genomics annotation and comparative studies of secondary metabolite biosynthetic gene clusters of the kingdom Bacteria[D]. Jinan:Shandong University, 2016.
[29]	Jeske O, Jogler M, Petersen J, et al. From genome mining to phenotypic microarrays:Planctomycetesas source for novel bioactive molecules[J]. Antonie van Leeuwenhoek, 2013,104(4):551-567. URL pmid: 23982431

物种	菌株	组装水平	基因组大小/Mb	GC/%	基因	蛋白	描述	登录号
Bacillus coagulans	S-lac	CompleteGenome	3.69484	46.2	3 559	3247	信息缺失	CP011939.1
	IDCC1201	CompleteGenome	3.66421	46.2	3 537	3 237	从韩国华城麦芽中分离	CP035305.1
	LA204	CompleteGenome	3.64257	46.2	3 618	3 327	从武汉玉米田土壤中分离	CP025437.1
	DSM2314	CompleteGenome	3.62865	46.2	3 515	3 231	从根际分离	CP033687.1
	HM-08	CompleteGenome	3.62464	46.3	3 439	3 192	从鸡肠道中分离	CP010525.1
	R11	CompleteGenome	3.60275	46.3	3 568	3 228	从中国云南泥土中分离	CP026649.1
	BC-HY1	CompleteGenome	3.59908	46.2	3 427	3 181	从猪消化道中分离	CP017888.1
	36D1	CompleteGenome	3.55223	46.5	3 414	3 140	信息缺失	CP003056.1
	2-6	CompleteGenome	3.07308	47.3	3 062	2 778	信息缺失	CP002472.1
	LBSC	Chromosome	3.6359	46.3	3 627	3 332	从印度纳西克泥土中分离	CP022701.1
	DSM 1	Contig	3.01804	47.2	3 039	2 779	信息缺失	ALAS00000000.1
	DSM 1	scaffold	2.95252	47.3	3 124	2 788	信息缺失	FQUB00000000.1
	ATCC7050	Contig	2.94782	47.3	3 028	2 754	信息缺失	ATUM00000000.1
	ZB29	scaffold	3.64647	46.2	3 660	3 213	从印度孟买牛奶中分离	QPFA00000000.1
	XZL9	Contig	3.42411	46.5	3 275	3 084	从中国济南泥土中分离	ANAP00000000.1
	NL01	Contig	3.50142	46.2	3 426	3 176	从中国南京泥土中分离	LBMQ00000000.1
	AF24-19	scaffold	2.9104	47.4	2 958	2 703	从人体粪便中分离	QRUW00000000.1
	MGYG-HG UT-00191	scaffold	2.9104	47.4	2 956	2 701	从人体肠道中分离	CABJCT000000000.1
	P38	Contig	3.37432	46.4	3 303	3 098	从中国泥土中分离	JSVI00000000.1
	AF24-21	scaffold	2.89159	47.5	2 890	2 699	从人体粪便分离	QRUS00000000.1
	Unique IS-2	Contig	3.44669	46.4	3 322	3 128	从被人体粪便污染的土壤中分离	JZDH00000000.1
	CSIL1	scaffold	3.46623	46.3	3 358	3 121	信息缺失	AXVW00000000.1
	HS243	Contig	3.4563	46.4	3 429	2 732	从印度浦那马哈拉施特拉邦商业食品中分离获取	NFZN00000000.1
	GBI-30,6086	Contig	3.45862	46.4	3 384	3 133	从冻干粉中分离获得	JPSK00000000.1
	B4100	Scaffold	3.42452	46.5	3 350	3 108	从意大利乳清干酪中分离	LQYH00000000.1
	GED7749B	Scaffold	3.31243	46.5	3 150	3 017	从阴道中分离获取	LRPN00000000.1
	B4098	Scaffold	2.9287	47.4	2 952	2 705	从中国番茄罐头中分离	LQYG00000000.1
	B4096	scaffold	3.3136	46.5	3 184	2 994	从中国番茄罐头中分离	LQYJ00000000.1
	MA-13	Contig	2.05947	46.9	2 064	1 804	从农业废弃物中分离	SMSP00000000.1
	XZL4	scaffold	2.86628	47.4	2 818	2 558	信息缺失	AFWM00000000.1
	B4099	scaffold	3.27533	47.1	3 287	2 999	从印度咖喱中分离	LQYI00000000.1
	H-1	Contig	2.86288	47.3	2 788	2 513	从上海泥土中分离	ANAQ00000000.1
	ATCC 7050	CompleteGenome	3.367	46.9	3 405	3 253	从爱荷华州乳制品中分离	CP009709.1

物种	菌株	组装水平	基因组大小/Mb	GC/%	基因	蛋白	描述	登录号
Bacillus coagulans	S-lac	CompleteGenome	3.69484	46.2	3 559	3247	信息缺失	CP011939.1
	IDCC1201	CompleteGenome	3.66421	46.2	3 537	3 237	从韩国华城麦芽中分离	CP035305.1
	LA204	CompleteGenome	3.64257	46.2	3 618	3 327	从武汉玉米田土壤中分离	CP025437.1
	DSM2314	CompleteGenome	3.62865	46.2	3 515	3 231	从根际分离	CP033687.1
	HM-08	CompleteGenome	3.62464	46.3	3 439	3 192	从鸡肠道中分离	CP010525.1
	R11	CompleteGenome	3.60275	46.3	3 568	3 228	从中国云南泥土中分离	CP026649.1
	BC-HY1	CompleteGenome	3.59908	46.2	3 427	3 181	从猪消化道中分离	CP017888.1
	36D1	CompleteGenome	3.55223	46.5	3 414	3 140	信息缺失	CP003056.1
	2-6	CompleteGenome	3.07308	47.3	3 062	2 778	信息缺失	CP002472.1
	LBSC	Chromosome	3.6359	46.3	3 627	3 332	从印度纳西克泥土中分离	CP022701.1
	DSM 1	Contig	3.01804	47.2	3 039	2 779	信息缺失	ALAS00000000.1
	DSM 1	scaffold	2.95252	47.3	3 124	2 788	信息缺失	FQUB00000000.1
	ATCC7050	Contig	2.94782	47.3	3 028	2 754	信息缺失	ATUM00000000.1
	ZB29	scaffold	3.64647	46.2	3 660	3 213	从印度孟买牛奶中分离	QPFA00000000.1
	XZL9	Contig	3.42411	46.5	3 275	3 084	从中国济南泥土中分离	ANAP00000000.1
	NL01	Contig	3.50142	46.2	3 426	3 176	从中国南京泥土中分离	LBMQ00000000.1
	AF24-19	scaffold	2.9104	47.4	2 958	2 703	从人体粪便中分离	QRUW00000000.1
	MGYG-HG UT-00191	scaffold	2.9104	47.4	2 956	2 701	从人体肠道中分离	CABJCT000000000.1
	P38	Contig	3.37432	46.4	3 303	3 098	从中国泥土中分离	JSVI00000000.1
	AF24-21	scaffold	2.89159	47.5	2 890	2 699	从人体粪便分离	QRUS00000000.1
	Unique IS-2	Contig	3.44669	46.4	3 322	3 128	从被人体粪便污染的土壤中分离	JZDH00000000.1
	CSIL1	scaffold	3.46623	46.3	3 358	3 121	信息缺失	AXVW00000000.1
	HS243	Contig	3.4563	46.4	3 429	2 732	从印度浦那马哈拉施特拉邦商业食品中分离获取	NFZN00000000.1
	GBI-30,6086	Contig	3.45862	46.4	3 384	3 133	从冻干粉中分离获得	JPSK00000000.1
	B4100	Scaffold	3.42452	46.5	3 350	3 108	从意大利乳清干酪中分离	LQYH00000000.1
	GED7749B	Scaffold	3.31243	46.5	3 150	3 017	从阴道中分离获取	LRPN00000000.1
	B4098	Scaffold	2.9287	47.4	2 952	2 705	从中国番茄罐头中分离	LQYG00000000.1
	B4096	scaffold	3.3136	46.5	3 184	2 994	从中国番茄罐头中分离	LQYJ00000000.1
	MA-13	Contig	2.05947	46.9	2 064	1 804	从农业废弃物中分离	SMSP00000000.1
	XZL4	scaffold	2.86628	47.4	2 818	2 558	信息缺失	AFWM00000000.1
	B4099	scaffold	3.27533	47.1	3 287	2 999	从印度咖喱中分离	LQYI00000000.1
	H-1	Contig	2.86288	47.3	2 788	2 513	从上海泥土中分离	ANAQ00000000.1
	ATCC 7050	CompleteGenome	3.367	46.9	3 405	3 253	从爱荷华州乳制品中分离	CP009709.1

菌株	登录号	基因簇	类型	长度	起始点	终止点
S-lac	CP011939.1	Cluster1	bacteriocin	10 212	244 719	254 931
	CP011939.1	Cluster2	betalactone	28 500	421 549	450 049
	CP011939.1	Cluste3	T3PKS	41 184	1 368 873	1 410 057
IDCC1201	CP035305.1	Cluster4	bacteriocin	9 164	1 948 175	1 957 339
	CP035305.1	Cluster5	betalactone	28 500	2 124 507	2 153 007
	CP035305.1	Cluster6	bacteriocin	10 227	2 645 063	2 655 290
	CP035305.1	Cluster7	T3PKS	41 184	3 073 385	3 114 569
LA204	CP025437.1	Cluster8	LAP	23 104	89 293	112 397
	CP025437.1	Cluster9	bacteriocin	9 627	2 744 552	2 754 179
	CP025437.1	Cluster10	bacteriocin	9 476	2 817 877	2 827 353
	CP025437.1	Cluster11	betalactone	27 796	3 021 109	3 048 905
	CP025437.1	Cluster12	bacteriocin	10 227	3 511 998	3 522 225
DSM 2314	CP033687.1	Cluster13	bacteriocin	9 627	250 405	260 032
	CP033687.1	Cluster14	bacteriocin	9 476	323 727	333 203
	CP033687.1	Cluster15	betalactone	27 796	529 699	557 495
	CP033687.1	Cluster16	bacteriocin	10 227	1 018 620	1 028 847
	CP033687.1	Cluster17	LAP	23 104	1 238 486	1 261 590
HM-08	CP010525.1	Cluster18	betalactone	28 500	639 210	667 710
	CP010525.1	Cluster19	bacteriocin	10 212	834 035	844 247
R11	CP026649.1	Cluster20	bacteriocin	9 667	2 771 450	2 781 117
	CP026649.1	Cluster21	betalactone	28 570	2 954 738	2 983 308
	CP026649.1	Cluster22	bacteriocin	10 227	3 478 574	3 488 801
BC-HY1	CP017888.1	Cluster23	thiopeptide	25 017	362 914	387 931
	CP017888.1	Cluster24	bacteriocin	9 471	1 965 274	1 974 745
	CP017888.1	Cluster25	betalactone	28 381	2 194 844	2 223 225
	CP017888.1	Cluster26	terpene	21 865	2 236 543	2 258 408
	CP017888.1	Cluster27	bacteriocin	10 227	2 706 642	2 716 869
36D1	CP003056.1	Cluster28	betalactone	28 255	2 024 346	2 052 601
	CP003056.1	Cluster29	bacteriocin	10 338	2 271 308	2 281 646
2-6	CP002472.1	Cluster30	T3PKS	41 172	187 665	228 837
LBSC	CP022701.1	Cluster31	bacteriocin	9 775	138 515	148 290
	CP022701.1	Cluster32	betalactone	28 500	642 143	670 643
	CP022701.1	Cluster33	bacteriocin	10 338	836 553	846 891
DSM 1_1	FQUB01000013.1	Cluster34	T3PKS	26 788	29 638	56 426
ZB29	QPFA01000001.1	Cluster35	bacteriocin	9 352	1 563 368	1 572 720
	QPFA01000001.1	Cluster36	betalactone	28 500	1 742 415	1 770 915
	QPFA01000001.1	Cluster37	bacteriocin	10 227	2 227 614	2 237 841
AF24-19	QRUW01000037.1	Cluster38	T3PKS	25 287	1	25 288
P38	JSVI01000006.1	Cluster39	betalactone	28 246	27 219	55 465
	JSVI01000047.1	Cluster40	bacteriocin	10 059	25 667	35 726
	JSVI01000063.1	Cluster41	bacteriocin	8 694	12 239	20 933
AF24-21	QRUS01000001.1	Cluster42	saccharide	24 880	3 728	28 608
	QRUS01000001.1	Cluster43	saccharide	31 154	94 433	125 587
	QRUS01000004.1	Cluster44	saccharide	24 939	29 550	54 489
	QRUS01000004.1	Cluster45	saccharide	36 214	57 428	93 642
	QRUS01000006.1	Cluster46	saccharide	54 277	3 383	57 660
	QRUS01000007.1	Cluster47	fatty_acid	22 247	56 035	78 282
	QRUS01000008.1	Cluster48	saccharide	35 116	60 649	95 765
	QRUS01000017.1	Cluster49	saccharide	41 249	5 918	47 167
	QRUS01000018.1	Cluster50	saccharide	21 642	24 679	46 321
	QRUS01000023.1	Cluster51	saccharide	30 277	8 181	38 458
	QRUS01000024.1	Cluster52	saccharide	35 431	4 125	39 556
	QRUS01000030.1	Cluster53	saccharide	30 072	2 908	32 980
	QRUS01000039.1	Cluster54	T3PKS	25 044	1	25 045
	QRUS01000042.1	Cluster55	fatty_acid	16 223	2 469	18 692
Unique IS-2	JZDH01000003.1	Cluster56	bacteriocin	10 227	3 494	13 721
	JZDH01000012.1	Cluster57	betalactone	27 244	1	27 245
	JZDH01000012.1	Cluster58	bacteriocin	5 397	193 633	199 030
HS243	NFZN01000010.1	Cluster59	betalactone	27 523	41 565	69 088
	NFZN01000014.1	Cluster60	bacteriocin	10 227	18 875	29 102
	NFZN01000026.1	Cluster61	bacteriocin	5 525	42 873	48 398
GBI-30,6086	JPSK01000068.1	Cluster62	bacteriocin	10 227	18 640	28 867
	JPSK01000095.1	Cluster63	betalactone	16 648	1	16 649
	JPSK01000097.1	Cluster64	bacteriocin	5 435	42 803	48 238
B4100	LQYH01000015.1	Cluster65	betalactone	27 796	4 691	32 487
	LQYH01000022.1	Cluster66	bacteriocin	10 338	9 516	19 854
	LQYH01000044.1	Cluster67	thiopeptide	25 017	54 079	79 096
GED7749B	KQ955874.1	Cluster68	betalactone	15 851	1	15 852
	KQ955875.1	Cluster69	bacteriocin	10 338	49 800	60 138
	KQ955921.1	Cluster70	bacteriocin	5 337	1	5 338
	KQ955931.1	Cluster71	thiopeptide	7 473	1	7 474
B4098	LQYG01000055.1	Cluster72	T3PKS	23 277	21 835	45 112
B4096	LQYJ01000010.1	Cluster73	betalactone	23 669	17 246	40 915
	LQYJ01000039.1	Cluster74	LAP	15 866	67 480	83 346
	LQYJ01000041.1	Cluster75	T3PKS	64 83	1	64 84
B4099	LQYI01000035.1	Cluster76	terpene	12 783	8 502	21 285
	LQYI01000062.1	Cluster77	T3PKS	7 228	1	7 229
	LQYI01000073.1	Cluster78	betalactone	20 280	1	20 281
ATCC 7050	CP009709.1	Cluster79	T3PKS	41 172	2 513 409	2 554 581

菌株	登录号	基因簇	类型	长度	起始点	终止点
S-lac	CP011939.1	Cluster1	bacteriocin	10 212	244 719	254 931
	CP011939.1	Cluster2	betalactone	28 500	421 549	450 049
	CP011939.1	Cluste3	T3PKS	41 184	1 368 873	1 410 057
IDCC1201	CP035305.1	Cluster4	bacteriocin	9 164	1 948 175	1 957 339
	CP035305.1	Cluster5	betalactone	28 500	2 124 507	2 153 007
	CP035305.1	Cluster6	bacteriocin	10 227	2 645 063	2 655 290
	CP035305.1	Cluster7	T3PKS	41 184	3 073 385	3 114 569
LA204	CP025437.1	Cluster8	LAP	23 104	89 293	112 397
	CP025437.1	Cluster9	bacteriocin	9 627	2 744 552	2 754 179
	CP025437.1	Cluster10	bacteriocin	9 476	2 817 877	2 827 353
	CP025437.1	Cluster11	betalactone	27 796	3 021 109	3 048 905
	CP025437.1	Cluster12	bacteriocin	10 227	3 511 998	3 522 225
DSM 2314	CP033687.1	Cluster13	bacteriocin	9 627	250 405	260 032
	CP033687.1	Cluster14	bacteriocin	9 476	323 727	333 203
	CP033687.1	Cluster15	betalactone	27 796	529 699	557 495
	CP033687.1	Cluster16	bacteriocin	10 227	1 018 620	1 028 847
	CP033687.1	Cluster17	LAP	23 104	1 238 486	1 261 590
HM-08	CP010525.1	Cluster18	betalactone	28 500	639 210	667 710
	CP010525.1	Cluster19	bacteriocin	10 212	834 035	844 247
R11	CP026649.1	Cluster20	bacteriocin	9 667	2 771 450	2 781 117
	CP026649.1	Cluster21	betalactone	28 570	2 954 738	2 983 308
	CP026649.1	Cluster22	bacteriocin	10 227	3 478 574	3 488 801
BC-HY1	CP017888.1	Cluster23	thiopeptide	25 017	362 914	387 931
	CP017888.1	Cluster24	bacteriocin	9 471	1 965 274	1 974 745
	CP017888.1	Cluster25	betalactone	28 381	2 194 844	2 223 225
	CP017888.1	Cluster26	terpene	21 865	2 236 543	2 258 408
	CP017888.1	Cluster27	bacteriocin	10 227	2 706 642	2 716 869
36D1	CP003056.1	Cluster28	betalactone	28 255	2 024 346	2 052 601
	CP003056.1	Cluster29	bacteriocin	10 338	2 271 308	2 281 646
2-6	CP002472.1	Cluster30	T3PKS	41 172	187 665	228 837
LBSC	CP022701.1	Cluster31	bacteriocin	9 775	138 515	148 290
	CP022701.1	Cluster32	betalactone	28 500	642 143	670 643
	CP022701.1	Cluster33	bacteriocin	10 338	836 553	846 891
DSM 1_1	FQUB01000013.1	Cluster34	T3PKS	26 788	29 638	56 426
ZB29	QPFA01000001.1	Cluster35	bacteriocin	9 352	1 563 368	1 572 720
	QPFA01000001.1	Cluster36	betalactone	28 500	1 742 415	1 770 915
	QPFA01000001.1	Cluster37	bacteriocin	10 227	2 227 614	2 237 841
AF24-19	QRUW01000037.1	Cluster38	T3PKS	25 287	1	25 288
P38	JSVI01000006.1	Cluster39	betalactone	28 246	27 219	55 465
	JSVI01000047.1	Cluster40	bacteriocin	10 059	25 667	35 726
	JSVI01000063.1	Cluster41	bacteriocin	8 694	12 239	20 933
AF24-21	QRUS01000001.1	Cluster42	saccharide	24 880	3 728	28 608
	QRUS01000001.1	Cluster43	saccharide	31 154	94 433	125 587
	QRUS01000004.1	Cluster44	saccharide	24 939	29 550	54 489
	QRUS01000004.1	Cluster45	saccharide	36 214	57 428	93 642
	QRUS01000006.1	Cluster46	saccharide	54 277	3 383	57 660
	QRUS01000007.1	Cluster47	fatty_acid	22 247	56 035	78 282
	QRUS01000008.1	Cluster48	saccharide	35 116	60 649	95 765
	QRUS01000017.1	Cluster49	saccharide	41 249	5 918	47 167
	QRUS01000018.1	Cluster50	saccharide	21 642	24 679	46 321
	QRUS01000023.1	Cluster51	saccharide	30 277	8 181	38 458
	QRUS01000024.1	Cluster52	saccharide	35 431	4 125	39 556
	QRUS01000030.1	Cluster53	saccharide	30 072	2 908	32 980
	QRUS01000039.1	Cluster54	T3PKS	25 044	1	25 045
	QRUS01000042.1	Cluster55	fatty_acid	16 223	2 469	18 692
Unique IS-2	JZDH01000003.1	Cluster56	bacteriocin	10 227	3 494	13 721
	JZDH01000012.1	Cluster57	betalactone	27 244	1	27 245
	JZDH01000012.1	Cluster58	bacteriocin	5 397	193 633	199 030
HS243	NFZN01000010.1	Cluster59	betalactone	27 523	41 565	69 088
	NFZN01000014.1	Cluster60	bacteriocin	10 227	18 875	29 102
	NFZN01000026.1	Cluster61	bacteriocin	5 525	42 873	48 398
GBI-30,6086	JPSK01000068.1	Cluster62	bacteriocin	10 227	18 640	28 867
	JPSK01000095.1	Cluster63	betalactone	16 648	1	16 649
	JPSK01000097.1	Cluster64	bacteriocin	5 435	42 803	48 238
B4100	LQYH01000015.1	Cluster65	betalactone	27 796	4 691	32 487
	LQYH01000022.1	Cluster66	bacteriocin	10 338	9 516	19 854
	LQYH01000044.1	Cluster67	thiopeptide	25 017	54 079	79 096
GED7749B	KQ955874.1	Cluster68	betalactone	15 851	1	15 852
	KQ955875.1	Cluster69	bacteriocin	10 338	49 800	60 138
	KQ955921.1	Cluster70	bacteriocin	5 337	1	5 338
	KQ955931.1	Cluster71	thiopeptide	7 473	1	7 474
B4098	LQYG01000055.1	Cluster72	T3PKS	23 277	21 835	45 112
B4096	LQYJ01000010.1	Cluster73	betalactone	23 669	17 246	40 915
	LQYJ01000039.1	Cluster74	LAP	15 866	67 480	83 346
	LQYJ01000041.1	Cluster75	T3PKS	64 83	1	64 84
B4099	LQYI01000035.1	Cluster76	terpene	12 783	8 502	21 285
	LQYI01000062.1	Cluster77	T3PKS	7 228	1	7 229
	LQYI01000073.1	Cluster78	betalactone	20 280	1	20 281
ATCC 7050	CP009709.1	Cluster79	T3PKS	41 172	2 513 409	2 554 581

基因簇	最相似已知基因簇	相似度	MIBiG 数据库登录号	基因簇	最相似已知基因簇	相似度	MIBiG 数据库登录号
Cluster2	Fengycin	40%	CP011939.1	Cluster38	rhizocticin A	6%	QRUW01000037.1
Cluster4	Amylocyclicin	66%	CP035305.1	Cluster39	Fengycin	40%	JSVI01000006.1
Cluster5	Fengycin	40%	CP035305.1	Cluster41	Amylocyclicin	66%	JSVI01000063.1
Cluster8	Listeriolysin S	37%	CP025437.1	Cluster42	tauramamide	13%	QRUS01000001.1
Cluster10	Amylocyclicin	66%	CP025437.1	Cluster43	dipeptide aldehydes	11%	QRUS01000001.1
Cluster11	Fengycin	40%	CP025437.1	Cluster46	molybdenum cofactor	17%	QRUS01000006.1
Cluster14	Amylocyclicin	66%	CP033687.1	Cluster47	chejuenolide A/	7%	QRUS01000007.1
Cluster15	Fengycin	40%	CP033687.1		chejuenolide B
Cluster17	Listeriolysin S	37%	CP033687.1	Cluster51	exopolysaccharide	10%	QRUS01000023.1
Cluster18	Fengycin	40%	CP010525.1	Cluster54	rhizocticin A	6%	QRUS01000039.1
Cluster19	Amylocyclicin	66%	CP010525.1	Cluster57	Fengycin	40%	JZDH01000012.1
Cluster20	Amylocyclicin	66%	CP026649.1	Cluster59	Fengycin	40%	NFZN01000010.1
Cluster21	Fengycin	40%	CP026649.1	Cluster63	Fengycin	40%	JPSK01000095.1
Cluster24	Amylocyclicin	66%	CP017888.1	Cluster65	Fengycin	40%	LQYH01000015.1
Cluster25	Fengycin	40%	CP017888.1	Cluster66	Amylocyclicin	50%	LQYH01000022.1
Cluster26	Kanamycin	1%	CP017888.1	Cluster68	Fengycin	40%	KQ955874.1
Cluster28	Fengycin	40%	CP003056.1	Cluster69	Amylocyclicin	66%	KQ955875.1
Cluster29	Amylocyclicin	66%	CP003056.1	Cluster73	Fengycin	40%	LQYJ01000010.1
Cluster32	Fengycin	40%	CP022701.1	Cluster74	Listeriolysin S	37%	LQYJ01000039.1
Cluster33	Amylocyclicin	66%	CP022701.1	Cluster76	Kanamycin	1%	LQYI01000035.1
Cluster35	Amylocyclicin	66%	QPFA01000001.1	Cluster78	Fengycin	40%	LQYI01000073.1
Cluster36	Fengycin	40%	QPFA01000001.1	Cluster79	rhizocticin A	6%	CP009709.1