组学技术在重离子辐射微生物诱变育种中的应用

doi:10.13560/j.cnki.biotech.bull.1985.2022-0958

摘要/Abstract

摘要：

重离子辐射诱变具有诱变率高、诱变谱宽、突变体易稳定等优势，在微生物育种实践中已得到广泛应用。随着测序技术的发展，对重离子辐射诱变效应的研究可以实现较为全面地了解突变体在基因组、转录组、蛋白质组和代谢组等多个层面的生物学信息。本文综述了利用重离子辐射诱变技术进行微生物育种的研究进展，以及联合高通量测序技术探究重离子诱变产生的生物学效应机理。在此基础上，进一步探讨利用组学方法研究重离子诱变微生物的新思路，旨为重离子诱变微生物育种技术提供参考和建议。

关键词: 重离子辐射, 微生物诱变育种, 多组学

Abstract:

Heavy ion beam（HIB）irradiation, with high mutagenesis rate, broad mutagenesis spectrum and stable mutant, has been widely used in the implementation of industrial microbial breeding. With the development of sequencing technology, a more comprehensive understanding of the biological information at multiple levels such as genome, transcriptome, proteome, and metabolome may be achieved via study on the mutagenesis effect of HIB. This paper summarizes the research progress of microbial breeding using HIB, combining high-throughput sequencing technology to explore the biological effects of heavy ion mutagenesis. On this basis, a new idea of using omics methods to study the mutagenesis effect of HIB was further discussed with aim to provide references and suggestions for the breeding technology.

Key words: heavy ion beam irradiation, microbial mutagenesis breeding, multi-omics

雷彩荣, 郭晓鹏, 柴冉, 张苗苗, 任军乐, 陆栋. 组学技术在重离子辐射微生物诱变育种中的应用[J]. 生物技术通报, 2023, 39(5): 54-62.

LEI Cai-rong, GUO Xiao-peng, CHAI Ran, ZHANG Miao-miao, REN Jun-le, LU Dong. Application of Omics Techniques in Incluced Breecling via Heavy Ion Beam Irradiating Microorganisms[J]. Biotechnology Bulletin, 2023, 39(5): 54-62.

图/表 2

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微生物菌株 Microorganism	离子类型 Ion beam	能量Energy /（MeV·u^-1）	突变结果 Mutation results	参考文献 Reference
链霉菌Streptomyces fungicidicus SG-01	¹²C⁶⁺	80	恩拉霉素产量增加43%	[5]
高山被孢霉菌Mortierella alpine SD003	¹²C⁶⁺	80	花生四烯酸产量达到 5.26 g/L	[7]
小花棘豆内生真菌Alternaria oxytropis UO1 酪丁酸梭菌Clostridium tyrobutyricum	¹²C⁶⁺ ¹²C⁶⁺	80 80	生物碱苦马豆素产量提高14.84% pH耐受性达到4.5和5.0	[10] [17]
谷氨酸棒状杆菌Corynebacterium Glutamicum GM006	¹²C⁶⁺	80	谷氨酸产量提高10.09%	[18]
丙酮丁醇梭菌C. acetobutylicum CICC 8012	¹²C⁶⁺	80	丁醇产量增加33%，丁醇抗性达到19 g/L	[19]
节杆菌Arthrobacter strain C1	¹²C⁶⁺	76.37	生物量达到129.84%，Cd²⁺抗性提高至100 mg/L	[20]
烟曲霉A. fumigatus MS13.1	¹²C⁶⁺	80	滤纸酶活性和葡萄糖苷酶活性分别提高 40.3%和15.1%	[21]
斜生栅藻Scenedesmus obliqnus SO120G	¹²C⁶⁺	80	脂质产量提高2.4倍	[22]
杜氏盐藻Dunaliella salina DS240G-1	¹²C⁶⁺	80	β-胡萝卜素含量增加21%	[23]

微生物菌株 Microorganism	离子类型 Ion beam	能量Energy /（MeV·u^-1）	突变结果 Mutation results	参考文献 Reference
链霉菌Streptomyces fungicidicus SG-01	¹²C⁶⁺	80	恩拉霉素产量增加43%	[5]
高山被孢霉菌Mortierella alpine SD003	¹²C⁶⁺	80	花生四烯酸产量达到 5.26 g/L	[7]
小花棘豆内生真菌Alternaria oxytropis UO1 酪丁酸梭菌Clostridium tyrobutyricum	¹²C⁶⁺ ¹²C⁶⁺	80 80	生物碱苦马豆素产量提高14.84% pH耐受性达到4.5和5.0	[10] [17]
谷氨酸棒状杆菌Corynebacterium Glutamicum GM006	¹²C⁶⁺	80	谷氨酸产量提高10.09%	[18]
丙酮丁醇梭菌C. acetobutylicum CICC 8012	¹²C⁶⁺	80	丁醇产量增加33%，丁醇抗性达到19 g/L	[19]
节杆菌Arthrobacter strain C1	¹²C⁶⁺	76.37	生物量达到129.84%，Cd²⁺抗性提高至100 mg/L	[20]
烟曲霉A. fumigatus MS13.1	¹²C⁶⁺	80	滤纸酶活性和葡萄糖苷酶活性分别提高 40.3%和15.1%	[21]
斜生栅藻Scenedesmus obliqnus SO120G	¹²C⁶⁺	80	脂质产量提高2.4倍	[22]
杜氏盐藻Dunaliella salina DS240G-1	¹²C⁶⁺	80	β-胡萝卜素含量增加21%	[23]