增加革兰阴性菌外膜囊泡产量的研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2023-0540

摘要/Abstract

摘要：

外膜囊泡（OMVs）是由革兰阴性菌释放的一种囊泡状结构，在革兰阴性菌的生长、感染以及与周围环境交流等过程中发挥重要作用。由于OMVs无活性、不能复制且具有良好的免疫原性，被广泛应用于新型疫苗的研究。但天然OMVs产量低，无法达到规模化生产的要求，OMVs产量提升是OMVs疫苗产业化要解决的关键核心技术问题。本文主要从细菌基因改造、生长培养条件优化和OMVs生产纯化工艺优化3个方面对提升OMVs产量的研究进行综述，以期为OMVs疫苗产业化研究开发提供参考。

关键词: 外膜囊泡, 疫苗开发, 生物发生机制, 产量增加机制

Abstract:

Outer membrane vesicles（OMVs）are vesicle-like structures released by gram-negative bacteria, and OMVs play an important role in gram-negative bacteria growth, infection and communication with the environment. Because OMVs are inactive, non-replicable and possess high immunogenicity, OMVs are widely applied for research and development of novel vaccines. However, the natural OMVs yield is too low to meet scale-up production. To increase OMVs yield is the key technical issue that should be resolved for scale-up production. We mainly review on three aspects for increasing OMVs yield, modification on bacterial genes, optimization on bacterial growth conditions and OMVs production and purification, aiming to provide informative reference for industrial research and development of OMVs vaccine.

Key words: outer membrane vesicles, vaccine development, biogenesis mechanism, yield enhancement mechanism

刘灿, 闫晓阳, 曾焱, 欧祥龙, 廖永洪. 增加革兰阴性菌外膜囊泡产量的研究进展[J]. 生物技术通报, 2024, 40(3): 100-108.

LIU Can, YAN Xiao-yang, ZENG Yan, OU Xiang-long, LIAO Yong-hong. Research Progress in Increasing Yield of Outer Membrane Vesicles from Gram Negative Bacteria[J]. Biotechnology Bulletin, 2024, 40(3): 100-108.

图/表 1

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