植物接头蛋白复合体TPC的研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2024-0947

生物技术通报 ›› 2025, Vol. 41 ›› Issue (7): 60-68.doi: 10.13560/j.cnki.biotech.bull.1985.2024-0947

植物接头蛋白复合体TPC的研究进展

王玉同¹^,²^,³(), 张颖慧¹^,², 徐梅⁴, 严旭⁴(), 赵菲佚¹^,²(), 田丹⁵

^1.天水师范学院生物工程与技术学院，天水 741001
^2.天水师范学院硫生物技术研究所，天水 741001
^3.甘肃省农业固体废弃物资源化利用重点实验室，天水 741001
^4.绍兴文理学院生命与环境科学学院，绍兴 312000
^5.华南农业大学生命科学学院，广州 510642

收稿日期:2024-09-28 出版日期:2025-07-26 发布日期:2025-07-22
通讯作者: ‍：‍赵菲佚，男，博士，教授，研究方向：植物分子生物学；E-mail: tspaulzhao@tsnu.edu.cn
严旭，男，博士，讲师，研究方向：植物网格蛋白介导的膜运输；E-mail: yanxu@usx.edu.cn
作者简介:王玉同，男，博士，讲师，研究方向：植物网格蛋白介导的膜运输；E-mail: yt_wang2023@163.com
基金资助:
国家自然科学基金项目(32200607);甘肃省科技计划资助项目(24JRRE005);天水师范学院科研项目(KYQ2023-16)

Research Progress in Adaptor Protein Complex TPC in Plants

WANG Yu-tong¹^,²^,³(), ZHANG Ying-hui¹^,², XU Mei⁴, YAN Xu⁴(), ZHAO Fei-yi¹^,²(), TIAN Dan⁵

^1.School of Bioengineering and Biotechnology, Tianshui Normal University, Tianshui 741001
^2.Institute of Sulfur Biotechnology, Tianshui Normal University, Tianshui 741001
^3.Gansu Key Laboratory of Utilization of Agricultural Solid Waste Resources, Tianshui 741001
^4.School of Life and Environmental Sciences, Shaoxing University, Shaoxing 312000
^5.College of Life Sciences, South China Agricultural University, Guangzhou 510642

Received:2024-09-28 Published:2025-07-26 Online:2025-07-22

摘要/Abstract

摘要：

网格蛋白介导的胞吞途径（clathrin-mediated endocytosis, CME）是真核细胞质膜蛋白进行胞吞的主要途径之一，在植物生长发育、营养吸收、胞内外信号转导、生长素极性运输和逆境响应中具有不可或缺的生物学功能。尽管网格蛋白为CME过程的核心蛋白，但其本身不含跨膜结构域，需要接头蛋白将其招募至质膜。已知动植物细胞中网格蛋白质膜招募主要依赖于接头蛋白复合体AP-2，但随着对植物接头蛋白复合体的深入研究，发现植物中还存在一类进化上比较古老的接头蛋白复合体TPC（TPLATE complex），且在植物的生长发育和CME过程中扮演重要角色。本文介绍了植物TPC的组成、结构和进化历程，分析了TPC亚基的结构域，综述了TPC的生物学功能，最后对TPC在植物胞吞作用和生长发育的研究进行了展望，以期为了解和阐明植物TPC在胞吞运输的分子机制提供新的思路和见解。

关键词: 接头蛋白复合体, TPC, 网格蛋白, 胞吞途径

Abstract:

Clathrin-mediated endocytosis (CME) is a predominant endocytic pathway for plasma membrane (PM) protein internalization in eukaryotic cells. In plants, CME is essential for growth and developmental processes, nutrient uptake, transduction between extra- and intra-cellular signals, polar auxin transport, and stress responses. Although clathrin is the core protein of the CME process, it lacks a transmembrane domain and relies on adaptor proteins to be recruited to the PM. It is known that the PM recruitment of clathrin mainly depends on the adaptor protein complex AP-2 in animal and plant cells. However, an increasing number of evidences on plant adaptor protein complexes show that there is also an evolutionary ancient adaptor protein complex TPC (TPLATE complex) in plants, which plays a critical role in plant growth and development as well as CME processes. In this review, we briefly introduce the composition, structure, and evolutionary history of TPC in plants. Then we analyze the domains of TPC subunits. Furthermore, we summarize the biological functions of TPC. Finally, we prospect the research on TPC in plant endocytosis and growth and development, aiming to provide new ideas and insights for understanding and clarifying the molecular mechanism of plant TPC in endocytic transport.

Key words: adaptor protein complex, TPC (TPLATE complex), clathrin, endocytosis

王玉同, 张颖慧, 徐梅, 严旭, 赵菲佚, 田丹. 植物接头蛋白复合体TPC的研究进展[J]. 生物技术通报, 2025, 41(7): 60-68.

WANG Yu-tong, ZHANG Ying-hui, XU Mei, YAN Xu, ZHAO Fei-yi, TIAN Dan. Research Progress in Adaptor Protein Complex TPC in Plants[J]. Biotechnology Bulletin, 2025, 41(7): 60-68.

图/表 2

参考文献 48

[1]	徐昌文, 钱虹萍, 罗鹏云, 等. 植物膜蛋白的囊泡转运及调控机制的研究进展 [J]. 科学通报, 2023, 68(7): 762-778.
	Xu CW, Qian HP, Luo PY, et al. Advances in vesicle trafficking of membrane proteins and their regulatory mechanisms [J]. Chin Sci Bull, 2023, 68(7): 762-778.
[2]	Aniento F, Sánchez de Medina Hernández V, Dagdas Y, et al. Molecular mechanisms of endomembrane trafficking in plants [J]. Plant Cell, 2022, 34(1): 146-173.
[3]	严旭, 徐梅, 王玉同, 等. 植物胞吞和胞吐的耦合调控 [J]. 植物学报, 2022, 57(3): 375-387.
	Yan X, Xu M, Wang YT, et al. Coupling regulation of endocytosis and exocytosis in plants [J]. Chin Bull Bot, 2022, 57(3): 375-387.
[4]	Kraus M, Pleskot R, Van Damme D. Structural and evolutionary aspects of plant endocytosis [J]. Annu Rev Plant Biol, 2024, 75(1): 521-550.
[5]	Reynolds GD, Wang C, Pan JW, et al. Inroads into internalization: five years of endocytic exploration [J]. Plant Physiol, 2018, 176(1): 208-218.
[6]	Kaksonen M, Roux A. Mechanisms of clathrin-mediated endocytosis [J]. Nat Rev Mol Cell Biol, 2018, 19(5): 313-326.
[7]	Van Damme D, Bouget FY, Van Poucke K, et al. Molecular dissection of plant cytokinesis and phragmoplast structure: a survey of GFP-tagged proteins [J]. Plant J, 2004, 40(3): 386-398.
[8]	Van Damme D, Coutuer S, De Rycke R, et al. Somatic cytokinesis and pollen maturation in Arabidopsis depend on TPLATE, which has domains similar to coat proteins [J]. Plant Cell, 2006, 18(12): 3502-3518.
[9]	Larson RT, Dacks JB, Barlow LD. Recent gene duplications dominate evolutionary dynamics of adaptor protein complex subunits in embryophytes [J]. Traffic, 2019, 20(12): 961-973.
[10]	Gadeyne A, Sánchez-Rodríguez C, Vanneste S, et al. The TPLATE adaptor complex drives clathrin-mediated endocytosis in plants [J]. Cell, 2014, 156(4): 691-704.
[11]	Yperman K, Wang J, Eeckhout D, et al. Molecular architecture of the endocytic TPLATE complex [J]. Sci Adv, 2021, 7(9): eabe7999.
[12]	Hirst J, Schlacht A, Norcott JP, et al. Characterization of TSET, an ancient and widespread membrane trafficking complex [J]. eLife, 2014, 3: e02866.
[13]	Zhang Y, Persson S, Hirst J, et al. Change your TPLATE, change your fate: plant CME and beyond [J]. Trends Plant Sci, 2015, 20(1): 41-48.
[14]	Yperman K, Papageorgiou AC, Merceron R, et al. Distinct EH domains of the endocytic TPLATE complex confer lipid and protein binding [J]. Nat Commun, 2021, 12(1): 3050.
[15]	Wang J, Yperman K, Grones P, et al. Conditional destabilization of the TPLATE complex impairs endocytic internalization [J]. Proc Natl Acad Sci USA, 2021, 118(15): e2023456118.
[16]	Grones P, De Meyer A, Pleskot R, et al. The endocytic TPLATE complex internalizes ubiquitinated plasma membrane cargo [J]. Nat Plants, 2022, 8(12): 1467-1483.
[17]	Wang J, Jiang QH, Pleskot R, et al. TPLATE complex-dependent endocytosis attenuates CLAVATA1 signaling for shoot apical meristem maintenance [J]. EMBO Rep, 2023, 24(9): e54709.
[18]	Bashline L, Li SD, Zhu XY, et al. The TWD40-2 protein and the AP2 complex cooperate in the clathrin-mediated endocytosis of cellulose synthase to regulate cellulose biosynthesis [J]. Proc Natl Acad Sci USA, 2015, 112(41): 12870-12875.
[19]	Dragwidge JM, Wang YN, Brocard L, et al. Biomolecular condensation orchestrates clathrin-mediated endocytosis in plants [J]. Nat Cell Biol, 2024, 26(3): 438-449.
[20]	Wang PW, Pleskot R, Zang JZ, et al. Plant AtEH/Pan1 proteins drive autophagosome formation at ER-PM contact sites with actin and endocytic machinery [J]. Nat Commun, 2019, 10(1): 5132.
[21]	Winkler J, De Meyer A, Mylle E, et al. Nanobody-dependent delocalization of endocytic machinery in Arabidopsis root cells dampens their internalization capacity [J]. Front Plant Sci, 2021, 12: 538580.
[22]	Wang J, Mylle E, Johnson A, et al. High temporal resolution reveals simultaneous plasma membrane recruitment of TPLATE complex subunits [J]. Plant Physiol, 2020, 183(3): 986-997.
[23]	Platre MP, Noack LC, Doumane M, et al. A combinatorial lipid code shapes the electrostatic landscape of plant endomembranes [J]. Dev Cell, 2018, 45(4): 465-480.e11.
[24]	Sánchez-Rodríguez C, Shi YY, Kesten C, et al. The cellulose synthases are cargo of the TPLATE adaptor complex [J]. Mol Plant, 2018, 11(2): 346-349.
[25]	曹梦醒, 王昊, 韩晓, 等. TPLATE复合体亚基TML调控拟南芥花粉发育的研究 [J]. 中国细胞生物学学报, 2018, 40(10): 1670-1676.
	Cao MX, Wang H, Han X, et al. TML, one subunit of TPLATE complex, regulates pollen development in Arabidopsis [J]. Chin J Cell Biol, 2018, 40(10): 1670-1676.
[26]	Russo G, Carotenuto G, Fiorilli V, et al. Ectopic activation of cortical cell division during the accommodation of arbuscular mycorrhizal fungi [J]. New Phytol, 2019, 221(2): 1036-1048.
[27]	Van Damme D, Gadeyne A, Vanstraelen M, et al. Adaptin-like protein TPLATE and clathrin recruitment during plant somatic cytokinesis occurs via two distinct pathways [J]. Proc Natl Acad Sci USA, 2011, 108(2): 615-620.
[28]	Dragwidge JM, VAN Damme D. Visualising endocytosis in plants: past, present, and future [J]. J Microsc, 2020, 280(2): 104-110.
[29]	Sigismund S, Lanzetti L, Scita G, et al. Endocytosis in the context-dependent regulation of individual and collective cell properties [J]. Nat Rev Mol Cell Biol, 2021, 22(9): 625-643.
[30]	Bar M, Aharon M, Benjamin S, et al. AtEHDs, novel Arabidopsis EH-domain-containing proteins involved in endocytosis [J]. Plant J, 2008, 55(6): 1025-1038.
[31]	Bar M, Sharfman M, Schuster S, et al. The coiled-coil domain of EHD2 mediates inhibition of LeEix2 endocytosis and signaling [J]. PLoS One, 2009, 4(11): e7973.
[32]	Dahhan DA, Reynolds GD, Cárdenas JJ, et al. Proteomic characterization of isolated Arabidopsis clathrin-coated vesicles reveals evolutionarily conserved and plant-specific components [J]. Plant Cell, 2022, 34(6): 2150-2173.
[33]	Johnson A, Dahhan DA, Gnyliukh N, et al. The TPLATE complex mediates membrane bending during plant clathrin-mediated endocytosis [J]. Proc Natl Acad Sci USA, 2021, 118(51): e2113046118.
[34]	Henry GD, Corrigan DJ, Dineen JV, et al. Charge effects in the selection of NPF motifs by the EH domain of EHD1 [J]. Biochemistry, 2010, 49(16): 3381-3392.
[35]	Wang C, Hu TW, Yan X, et al. Differential regulation of clathrin and its adaptor proteins during membrane recruitment for endocytosis [J]. Plant Physiol, 2016, 171(1): 215-229.
[36]	Lamark T, Johansen T. Mechanisms of selective autophagy [J]. Annu Rev Cell Dev Biol, 2021, 37: 143-169.
[37]	Tang J, Bassham DC. Autophagy during drought: function, regulation, and potential application [J]. Plant J, 2022, 109(2): 390-401.
[38]	Wang PW, Hawes C, Hussey PJ. Plant endoplasmic reticulum-plasma membrane contact sites [J]. Trends Plant Sci, 2017, 22(4): 289-297.
[39]	张红红, 方晓峰. 相分离调控植物胁迫感知和应答的研究进展 [J]. 生物技术通报, 2023, 39(11): 44-53.
	Zhang HH, Fang XF. Advances in the regulation of stress sensing and responses by phase separation in plants [J]. Biotechnol Bull, 2023, 39(11): 44-53.
[40]	Donaldson JG. Phospholipase D in endocytosis and endosomal recycling pathways [J]. Biochim Biophys Acta, 2009, 1791(9): 845-849.
[41]	Ory S, Ceridono M, Momboisse F, et al. Phospholipid scramblase-1-induced lipid reorganization regulates compensatory endocytosis in neuroendocrine cells [J]. J Neurosci, 2013, 33(8): 3545-3556.
[42]	何世明, 王实, 吝易. 生物大分子相分离领域的研究进展回顾与展望 [J]. 科学通报, 2024, 69(30): 4486-4499.
	He SM, Wang S, Lin Y. Biomolecular phase separation research: milestones, insights, and future trajectories [J]. Chin Sci Bull, 2024, 69(30): 4486-4499.
[43]	Day KJ, Kago G, Wang LP, et al. Liquid-like protein interactions catalyse assembly of endocytic vesicles [J]. Nat Cell Biol, 2021, 23(4): 366-376.
[44]	Kozak M, Kaksonen M. Condensation of Ede1 promotes the initiation of endocytosis [J]. eLife, 2022, 11: e72865.
[45]	Fan LS, Li RL, Pan JW, et al. Endocytosis and its regulation in plants [J]. Trends Plant Sci, 2015, 20(6): 388-397.
[46]	Dahhan DA, Bednarek SY. Advances in structural, spatial, and temporal mechanics of plant endocytosis [J]. FEBS Lett, 2022, 596(17): 2269-2287.
[47]	Thompson B, Petrić Howe N. Alphafold 3.0: the AI protein predictor gets an upgrade [J]. Nature, 2024. DOI: 10.1038/d41586-024-01385-x .
[48]	Nomburg J, Doherty EE, Price N, et al. Birth of protein folds and functions in the virome [J]. Nature, 2024, 633(8030): 710-717.

亚基名称 Names of subunits	基因编号 Gene ID	突变体表型 Mutant phenotype	亚细胞定位 Subcellular localization	结构域 Structural domain	参与的运输途径 Involved in trafficking pathways	参考文献 References
TPLATE	At3g01780	花粉致死；下调导致幼苗根尖长度变短；根尖扭曲生长；部分功能缺失突变体对CLV3小肽表现出较强的敏感性	质膜	a-Solenoid， appendage， anchor， armadillo	（1）调控网格蛋白介导的质膜蛋白从质膜到反式高尔基体网络/早期内吞体的运输；（2）参与自噬途径	［10， 15， 17］
TML	At5g57460	花粉致死；下调导致幼苗根尖长度变短；根尖扭曲生长	质膜	μ-Homology， longin	（1）调控网格蛋白介导的质膜蛋白从质膜到反式高尔基体网络/早期内吞体的运输	［10］
TASH3	At2g07360	花粉致死；SH3结构域功能缺失而获得nosh突变体，该突变并不能阻止TPC的形成，却严重损害了胞吞作用以及植物生长发育	质膜	α-Solenoid， SH3， armadillo	（1）调控网格蛋白介导的质膜蛋白从质膜到反式高尔基体网络/早期内吞体的运输	［10， 16］
TWD40-1	At3g50590	花粉致死	质膜	β-Propeller， a-solenoid， WD40	（1）调控网格蛋白介导的质膜蛋白从质膜到反式高尔基体网络/早期内吞体的运输	［10］
TWD40-2	At5g24710	花粉致死；基因下调突变体表现更短的下胚轴表型；活性弱等位基因突变体	质膜	β-Propeller， a-solenoid， WD40	（1）调控网格蛋白介导的质膜蛋白从质膜到反式高尔基体网络/早期内吞体的运输	［10， 18］
AtEH1/Pan1	At1g20760	花粉致死	质膜；自噬体	EH， coiled coil	（1）调控网格蛋白介导的质膜蛋白从质膜到反式高尔基体网络/早期内吞体的运输；（2）参与肌动蛋白介导的自噬途径；（3）参与液-液相分离过程	［10， 19-20］
AtEH2/Pan1	At1g21630	花粉致死	质膜；自噬体	EH， coiled coil	（1）调控网格蛋白介导的质膜蛋白从质膜到反式高尔基体网络/早期内吞体的运输；（2）参与肌动蛋白介导的自噬途径；（3）参与液-液相分离过程	［10， 19-20］
LOLITA	At1g15370	花粉致死	质膜	Longin	（1）调控网格蛋白介导的质膜蛋白从质膜到反式高尔基体网络/早期内吞体的运输	［10］

亚基名称 Names of subunits	基因编号 Gene ID	突变体表型 Mutant phenotype	亚细胞定位 Subcellular localization	结构域 Structural domain	参与的运输途径 Involved in trafficking pathways	参考文献 References
TPLATE	At3g01780	花粉致死；下调导致幼苗根尖长度变短；根尖扭曲生长；部分功能缺失突变体对CLV3小肽表现出较强的敏感性	质膜	a-Solenoid， appendage， anchor， armadillo	（1）调控网格蛋白介导的质膜蛋白从质膜到反式高尔基体网络/早期内吞体的运输；（2）参与自噬途径	［10， 15， 17］
TML	At5g57460	花粉致死；下调导致幼苗根尖长度变短；根尖扭曲生长	质膜	μ-Homology， longin	（1）调控网格蛋白介导的质膜蛋白从质膜到反式高尔基体网络/早期内吞体的运输	［10］
TASH3	At2g07360	花粉致死；SH3结构域功能缺失而获得nosh突变体，该突变并不能阻止TPC的形成，却严重损害了胞吞作用以及植物生长发育	质膜	α-Solenoid， SH3， armadillo	（1）调控网格蛋白介导的质膜蛋白从质膜到反式高尔基体网络/早期内吞体的运输	［10， 16］
TWD40-1	At3g50590	花粉致死	质膜	β-Propeller， a-solenoid， WD40	（1）调控网格蛋白介导的质膜蛋白从质膜到反式高尔基体网络/早期内吞体的运输	［10］
TWD40-2	At5g24710	花粉致死；基因下调突变体表现更短的下胚轴表型；活性弱等位基因突变体	质膜	β-Propeller， a-solenoid， WD40	（1）调控网格蛋白介导的质膜蛋白从质膜到反式高尔基体网络/早期内吞体的运输	［10， 18］
AtEH1/Pan1	At1g20760	花粉致死	质膜；自噬体	EH， coiled coil	（1）调控网格蛋白介导的质膜蛋白从质膜到反式高尔基体网络/早期内吞体的运输；（2）参与肌动蛋白介导的自噬途径；（3）参与液-液相分离过程	［10， 19-20］
AtEH2/Pan1	At1g21630	花粉致死	质膜；自噬体	EH， coiled coil	（1）调控网格蛋白介导的质膜蛋白从质膜到反式高尔基体网络/早期内吞体的运输；（2）参与肌动蛋白介导的自噬途径；（3）参与液-液相分离过程	［10， 19-20］
LOLITA	At1g15370	花粉致死	质膜	Longin	（1）调控网格蛋白介导的质膜蛋白从质膜到反式高尔基体网络/早期内吞体的运输	［10］

植物接头蛋白复合体TPC的研究进展

Research Progress in Adaptor Protein Complex TPC in Plants

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 2

参考文献 48

相关文章 1

编辑推荐

Metrics

本文评价