外源甲状腺激素对牙鲆IGF-I及其受体基因表达的影响

生物技术通报 ›› 2013, Vol. 0 ›› Issue (11): 117-123.

外源甲状腺激素对牙鲆IGF-I及其受体基因表达的影响

张俊玲, 施志仪, 程琦, 付元帅

上海海洋大学农业部淡水渔业水产种质资源重点实验室，上海 201306

收稿日期:2013-05-16 出版日期:2013-11-14 发布日期:2013-11-14
作者简介:张俊玲，女，博士，副教授，研究方向：鱼类发育生物学及遗传育种；E-mail：jlzhang@shou.edu.cn
基金资助:
国家自然科学基金项目(31172392，41306128)，上海市重点学科海洋生物学建设项目(J50701)，上海海洋大学博士启动基金项目(B-8812-11-0194)

The Effects of Exogenous Thyroid Hormone on Gene Expression of IGF-I and IGF-IRs in Paralichthys olivaceus

Zhang Junling, Shi Zhiyi, Cheng Qi, Fu Yuanshuai

(Key laboratory of Freshwater Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Agriculture, Shanghai 201306)

Received:2013-05-16 Published:2013-11-14 Online:2013-11-14

摘要/Abstract

摘要： 采用外源的甲状腺激素(thyroid hormone，TH)分别处理变态前牙鲆仔鱼和体外培养的牙鲆胚胎细胞，通过实时定量PCR检测了TH处理前后牙鲆胰岛素样生长因子I(insulin-like growth factor I，IGF-I)及其受体(IGF-I receptors，IGF-IRs)基因的表达变化。在体和离体试验结果显示，过高浓度的TH会降低牙鲆仔鱼和胚胎细胞中IGF-I mRNA的表达，通过添加TH抑制剂硫脲(thiourea，TU)使仔鱼体内IGF-I mRNA上调；两种不同的IGF-IR对TH的响应是不同的，外源的TH能促进IGF-IR-1 mRNA的表达，却使IGF-IR-2 mRNA的表达下调。结果表明在牙鲆的生长发育中TH和IGF系统是高度相关的。

关键词: 牙鲆, IGF-I, IGF-I受体, 甲状腺激素

Abstract: In Paralichthys olivaceus, the gene expressions of insulin-like growth factor I(IGF-I)and its receptors after thyroid hormone(TH)treatment were analyzed using real-time quantitative PCR, by administration of TH to premetamorphic larvae and cultured embryonic cells. The results in vivo and in vitro showed that the level of IGF-I mRNA dropped significantly after TH treatment, and it was up-regulated in thiourea(TU)treated premetamorphic larvae. The two IGF-IR mRNAs exhibited different expression patterns after TH treatment. The exogenous TH stimulated the expression of IGF-IR-1 mRNA, but obviously decreased the levels of IGF-IR-2 mRNA. These results reveal that TH and IGF systems are highly interrelated and provide the basic information to clarify the hormone regulation mechanisms of metamorphosis in Paralichthys olivaceus.

Key words: Paralichthys olivaceus, IGF-I, IGF-I receptors, Thyroid hormone

张俊玲, 施志仪, 程琦, 付元帅. 外源甲状腺激素对牙鲆IGF-I及其受体基因表达的影响[J]. 生物技术通报, 2013, 0(11): 117-123.

Zhang Junling, Shi Zhiyi, Cheng Qi, Fu Yuanshuai. The Effects of Exogenous Thyroid Hormone on Gene Expression of IGF-I and IGF-IRs in Paralichthys olivaceus[J]. Biotechnology Bulletin, 2013, 0(11): 117-123.

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