AFLP分子标记在拟穴青蟹子一代家系中的遗传规律分析

摘要/Abstract

摘要：

采用AFLP技术对拟穴青蟹(Scylla paramamosain)子一代家系进行遗传学分析，并探讨了AFLP分子标记的遗传规律。32对ALFP选择性引物在拟穴青蟹家系G₁代中共扩增出1 128个位点，其中不分离位点752个，多态性位点376个，多态位点比例为33.3%。在376个分离的位点中，符合孟德尔分离规律的位点共229个，占分离位点总数的60.9%；偏分离位点147个，占分离位点总数的39.1%。分离比为1:1的位点共259个，占分离位点数的68.9%，其中符合孟德尔规律的位点155个，占59.8%；分离比为3:1的位点共有117个，占分离位点数的31.1%，符合孟德尔分离规律的位点74个，占63.3%。32对引物组合检测到的有效等位基因个数在1.13-1.73之间；Shannon指数在0.10-0.56之间；遗传多样性指数在0.07-0.40之间。

关键词: 拟穴青蟹, AFLP, 遗传多样性, 孟德尔分离

Abstract:

In this study, the segregating pattern of AFLP(amplified fragment length polymorphism)and the genetic diversity of G₁ family of the mud crab(Scylla paramamosain)was investigated using 32 selective primer combinations. A total of 1 128 loci were produced, of which 376 loci were polymorphic(11.8%), and 752 loci were monomorphic. Among 376 segregating loci, 229 loci(60.9%)segregated in Mendelian ratio while 147 loci(39.1%)segregated in distortion estimated by Chi-square test(P=0.05)．There were 259(68.9%)and 117(31.1%)loci segregating in the ratios of 1:1 and 3:1, respectively. The number of loci segregated in Mendelian ratios were 155(59.8%)for 1:1 and 74(63.3%)for 3:1, respectively. Effective number of alleles, Shannon genetic diversity index and Nei’s gene diversity index were between 1.13 to 1.73, between 0.10 to 0.56, and between 0.07 to 0.40, respectively.

Key words: Scylla paramamosain, AFLP, Genetic diversity, Mendelian segregation

李淑娟, 马洪雨, 马春艳, 蒋伟, 冯娜娜, 徐真, 刘月星, 乔振国, 马凌波. AFLP分子标记在拟穴青蟹子一代家系中的遗传规律分析[J]. 生物技术通报, 2013, 0(11): 123-129.

Li Shujuan, Ma Hongyu, Ma Chunyan, Jiang Wei, Feng Nana, Xu Zhen, Liu Yuexing, Qiao Zhenguo, Ma Lingbo^{1. Segregating Pattern of AFLP Marker in G₁ Family of the Mud Crab(Scylla paramamosain)[J]. Biotechnology Bulletin, 2013, 0(11): 123-129.}

参考文献

[1] 林琪, 李少菁, 黎中宝, 等.中国东南沿海青蟹属(Scylla)的种类组成[J].水产学报, 2007, 31(2)：211- 219.
[2] 王伟继, 岳志芹, 孔杰, 等. AFLP 分子标记技术的发展及其在海洋生物中的应用[J]. 海洋水产研究, 2005, 26(1)：80-85.
[3] 路心平, 马凌波, 乔振国, 等.利用线粒体DNA标记分析中国东南沿海拟穴青蟹种群遗传结构[J].水产学报, 2009, 1：15-23.
[4] 孙奉玉, 宋忠魁, 赵鹏, 等.广西沿海及其邻近海区拟穴青蟹群体遗传多样性的RAPD分析[J].南方水产科, 2012, 8(2)：30-35.
[5] 崔海玉, 马洪雨, 马春艳, 等.利用微卫星标记比较分析拟穴青蟹不同家系的遗传多样性[J].海洋渔业, 2011, 33(3)：274-281.
[6] 马群群, 马洪雨, 马春艳, 等.拟穴青蟹线粒体COI基因序列克隆及SNPs位点分析[J].生物技术通报, 2011(7)：111-116.
[7] Vos P, Hogers R, Bleeker M, et al. AFLP：a new technique forDNA fingerprinting[J]. Nucleic Acids Res, 1995, 23(21)：4407-4414.
[8] Liu YG, Chen SL, Li BF, et al. Analysis ofgenetic variation in selected stocks of hatcheryflounder, Paralichthys olivaceus, using AFLP markers[J]. Biochemical Systematics and Ecology, 2005, 33：993-1005.
[9] Mickett K, Morton C, Feng J, et al. Assessinggenetic diversity of domestic populations of channel catfish(Ictalurus punctatus)in Alabama using AFLP markers[J]. Aquaculture, 2003, 228：91-105.
[10] 韩志强, 高天翔, 王志, 等.黄姑鱼群体遗传多样性的AFLP分析[J].水产学报, 2006, 30(5)：640-646.
[11] Matsuda M, Nagahama Y, Shinomiya A, et al. DMY is a Y-specific DM-domain gene requiredfor male development in the medaka fish[J]. Nature, 2002, 417：559-563.
[12] Congiu L, Dupanloup I, Patarnello T, et al. Identification of interspecific hybrids by amplified fragment length polymorphism：thecase of sturgeon[J]. Molecular Ecology, 2000, 10：2355-2359.
[13] Young WP, Wheeler PA, Coryell VH, et al. A detailed linkage map of rainbow trout produced using doubled haploids[J]. Genetics, 1998, 148(2)：839-850.
[14] Kocher TD, Lee WJ, Sobolewska H, et al. A genetic linkage map of a cichlid fish, the tilapia(Oreochromis niloticus)[J]. Genetics, 1998, 148(3)：1225-1232.
[15] Agresti JJ, Seki S, Cnaani A, et al. Breeding new strains of tilapia：development of an artificial center of origin and linkage map based on AFLP and microsatellite loci[J]. Aquaculture, 2000, 185：43-56.
[16] Liu Z, Karsi A, Li P, et al. An AFLP based genetic linkage map of channel catfish(Ictalurus punctatus)constructed by using an interspecific hybrid resource family[J]. Genetics, 2003, 165(2)：687-694.
[17] Maria RMC, Kazunobu K, Shinichiro K, et al. A genetic linkage map of the Japanese flounder Paralichthys olivaceus[J]. Aquac-ulture, 2003, 220：203-218.
[18] Ezaz MT, Harvey SC, Boonphakdee C, et al. Isolation and physical mapping of sexlinked AFLP markers in Nile tilapia(Oreochromis niloticus L.)[J]. Marine Biotechnology, 2004, 5：435-445.
[19] Watanabe T, Fujita H, Yamasaki K, et al. Preliminary study on linkage mapping based on microsatellite DNA and AFLP markers using homozygous clones fish in ayu(Plecoglossus altivelis)[J]. Marine Biotechnology, 2004, 4：327-334.
[20] Sun X, Liang L. A genetic linkage map of common carp(Cyprinus carpio L.)and mapping of a locus associated with cold tolerance[J]. Aquaculture, 2004, 238：165-172.
[21] Darrin PR, Cheryl-Anne S, Melissa R, et al. A genetic linkage map of atlantic halibut(Hippoglossus hippoglossus L.)[J]. Genetics, 2007, 177：1193-1205.
[22] Wilson K, Li Y, Whan V, et al. Genetic mapping of the black tiger shrimp Penaeus monodon with amplified fragment length polymorphism[J]. Aquaculture, 2002, 204：297-309.
[23] Li Y, Byrne K, Miggiano E, et al. Genetic mapping of the kuruma prawn Penaeus japonicus using AFLP markers[J]. Aquaculture, 2003, 219：143-156.
[24] Pérez F, Erazo C, Zhinaula M, et al. A sex-specific linkage map of the white shrimp Penaeus(Litopenaeus)vannamei based on AFLP markers[J]. Aquaculture, 2004, 242：105-118.
[25] 王伟继, 孔杰, 董世瑞, 等.中国明对虾AFLP分子标记遗传连锁图谱的构建[J].动物学报, 2006, 52(3)：575-584.
[26] Li Z, Li J, Wang Q, et al. AFLP-based genetic linkage map of marine shrimp Penaeus(Fenneropenaeus)chinensis[J]. Aquaculture, 2006, 261：463-472.
[27] Zhang L, Yang C, Zhang Y, et al. A genetic linkage map of Pacific white shrimp(Litopenaeus vannamei)：sex-linked microsatellite markers and high recombination rates[J]. Genetics, 2007, 131(1)：37-49.
[28] 罗云, 高保全, 刘萍, 等.三疣梭子蟹遗传连锁图谱的初步构建[J].渔业科学进展, 2010, 31(3)：56-65.
[29] Yu Z, Guo X. Genetic linkage map of the eastern oyster, Crassostrea virginica Gmelin[J]. The Biological Bulletin, 2003, 204：327-338.
[30] Li L, Guo X. AFLP-based genetic linkage maps of the Pacific oyster Crassostrea gigas, Thunberg[J]. Marine Biotechnology, 2004, 6：26-36.
[31] Wang S, Bao Z, Pan J, et al. AFLP linkage map of an intraspecific cross in Chlamys farreri[J]. Shellfish Research, 2004, 23(2)：491-499.
[32] Wang L, Song L, Chang Y, et al. A preliminary genetic map of Zhikong scallop(Chlamys farreri Jones et Preston 1904)[J]. Aquaculture Research, 2005, 36(7)：643-653.
[33] Li L, Xiang J, Liu X, et al. Construction of AFLP-based genetic linkage map for Zhikong scallop, Chlamys farreri Jones et Preston and mapping of sexlinked markers[J]. Aquaculture, 2005, 245：63-73.
[34] Liu X, Liu X, Guo X, et al. A preliminary genetic linkage map of the pacific abalone Haliotis discushannai Ino[J]. Marine Biotechnology, 2006, 8(4)：386-397.
[35] Lallias D, Lapegue S, Hecquet C, et al. AFLP-based genetic linkage maps of the blue mussel(Mytilus edulis)[J]. Animal Genetics, 2007, 38：340-349.
[36] Wang L, Song L, Zhang H, et al. Genetic linkage map of bay scallop, Argopecten irradians irradians(Lamarck 1819)[J]. Aquaculture Research, 2007, 38：409-419.
[37] Zhou Z, Bao Z, Dong Y, et al. AFLP linkage map of sea urchin constructed using an interspecific cross between Strongylocentrotus nudus(♀)and S. intermedius(♂)[J]. Aquaculture, 2006, 259(1-4)：56-65.
[38] 杨天燕, 郭焱, 孟玮, 等.塔里木裂腹鱼群体遗传多样性的AFLP分析[J].淡水渔业, 2012, 42(4)：83-87.
[39] 罗云, 高保全, 刘萍, 等.三疣梭子蟹莱州湾、舟山野生个体定向交配产生F2代家系的AFLP分析[J].渔业科学进展, 2009, 30(6)：48-55.
[40] 李三磊, 徐冬冬, 楼宝, 等.舟山近海条石鲷野生群体与人工放流群体遗传多样性的AFLP[J].分析海洋科学, 2012, 36(8)：21-27.
[41] 王小玉, 喻达辉, 黄桂菊, 等.合浦珠母贝印度家系F1代的AFLP分析[J].中国水产科学, 2007, 14(1)：52-57.
[42] 岳志芹, 王伟继, 孔杰, 等. AFLP分子标记构建中国对虾遗传连锁图谱的初步研究[J].高技术通讯, 2004, 5：88-93.
[43] 常玉梅, 孙效文.水产养殖动物遗传连锁图谱及QTL定位研究进展[J].动物学研究, 2006, 27(5)：533-540.
[44] Sandler L, Novitski E. Meioticdrive as an evolutionary force[J]. American Naturalist, 1957, 91：105-110.
[45] 吴俊, 束怀瑞, 张开春, 等.桃分子连锁图的构建与分析[J].园艺学报, 2004, 31(5)：593-597.
[46] 易克, 许勇, 卢向阳, 等.西瓜重组自交系群体的AFLP分子图谱构建[J].园艺学报, 2004, 31(1)：53-58.
[47] 房经贵, 章镇, 马正强, 等. AFLP标记在两个芒果品种间杂交F1代的多态性及分离方式[J].中国农业科学, 2000, 33(3)：19-24.
[48] 司马杨虎, 陈大霞, 赵爱春, 等.家蚕AFLP分子标记分离规律的研究[J].蚕业科学, 2003, 29(2)：131-135.