生物技术通报 ›› 2014, Vol. 0 ›› Issue (1): 75-82.

• 技术与方法 • 上一篇    下一篇

酵母双杂交及其衍生系统

黄欣媛1,范红波2   

  1. 1. 湖北工程学院特色果蔬质量安全控制湖北省重点实验室,孝感 432000
    2. 湖北职业技术学院,孝感 432000
  • 收稿日期:2013-08-03 出版日期:2014-01-23 发布日期:2014-01-23
  • 作者简介:黄欣媛, 女, 博士, 研究方向: 生物化学与分子生物学;E-mail: huangxycn@gmail.com
  • 基金资助:
    特色果蔬质量安全控制湖北省重点实验室开放基金项目(2013K04)

The Yeast Two-Hybrid System and Its Several Derived Systems

Huang Xinyuan1, Fan Hongbo2   

  1. 1. Hubei Key Laboratory of Quality Control Huang Xinyuan1 Fan Hongbo of Characteristic Fruits and Vegetables,Hubei Engineering University,Xiaogan 432000;2.Hubei Polytechnic Institute,Xiaogan 432000
  • Received:2013-08-03 Published:2014-01-23 Online:2014-01-23

摘要: 酵母双杂交系统是一种研究蛋白质相互作用的分子生物学方法,过去20 多年里,大量衍生系统的出现使得这套双#br#杂交技术体系更加完善和高效,成为研究蛋白质-配体相互作用的重要技术手段,广泛应用于功能基因组学、蛋白质组学、病理学#br#等研究领域。对酵母双杂交及其衍生系统的基本原理和应用进展进行综述。

关键词: 酵母双杂交系统, 蛋白质相互作用, 双杂交技术体系

Abstract: The Yeast two-hybrid(Y2H)system is a molecular biology approach to detect protein-protein interactions. A diverse series of technologies derived from it have emerged in the past 20 years, which makes this two-hybrid methodology system more complete and efficient. They have been among the most important and powerful tools to study ptotein-ligand interactions that widely used in functional genomics,proteomics and pathology study. This article provides an overview on the basic principles and application progress of Y2H system and some derived techniques.

Key words: Yeast two-hybrid(Y2H)system, Protein-protein interaction, Two-hybrid methodology system