利用基因芯片技术鉴别山羊绒和绵羊绒

生物技术通报 ›› 2014, Vol. 0 ›› Issue (1): 196-200.

• 研究报告 • 上一篇

利用基因芯片技术鉴别山羊绒和绵羊绒

吕雪峰,阿布力孜?阿布力米提,帕娜尔,陶卫东,邢巍婷,采复拉,郑文新

农业部种羊与羊毛羊绒质量监督检验测试中心，乌鲁木齐 830000

收稿日期:2013-10-07 出版日期:2014-01-23 发布日期:2014-01-23
作者简介:吕雪峰，男，中级，研究方向: 动物遗传育种；E-mail: lxf00700@163.com
基金资助:
新疆维吾尔自治区公益性科研院所基本科研业务经费

Identification of Cashmere and Sheep down by Microarray Technology

Lü Xuefeng, Abliz Ablimit Pa Naer, Tao Weidong, Xing Weiting, Cai Fula, Zheng Wenxin

Department of Agriculture-Breeding Sheep and Wool and Cashmere Quality Supervision Test Center，Urumqi 830000

Received:2013-10-07 Published:2014-01-23 Online:2014-01-23

摘要/Abstract

摘要： 为了建立山羊绒与绵羊绒的基因芯片鉴别方法，达到快速、高通量鉴别山羊绒与绵羊绒的目的，文章根据山羊和#br#绵羊遗传物质的特异性，选择线粒体基因组细胞色素b 基因(cyt b)为目的基因，在cyt b 基因通用引物区间设计出一对能鉴别绵#br#羊绒与山羊绒的探针，经过与含有不同比例绵羊源性的PCR 产物杂交，结果显示当山羊源性成分中绵羊源性含量为3% 时，仍然#br#可见杂交信号，说明基因芯片鉴别毛绒的方法具有非常高的特异性，能够定性的鉴别山羊绒和绵羊绒。

Abstract: This study is to explore the method for identification of cashmere and sheep down by gene chip, thereby enabling rapid, highthroughput identification of cashmere and sheep down. According to the specificity of the genetic material of cashmere and sheep down, cyt b was selected as the target gene, a pair of probes that can identify cashmere and sheep down was designed in the universal primers interval of cyt b gene. Then PCR products of different proportions derived from sheep were hybridized with probe. Results showed that in goat derived PCR products, when content of PCR products derived from sheep was 3%, hybridization signal was still visible. It indicates that the gene chip method can be used to qualitatively identify the cashmere and sheep down, and has a very high specificity.

Key words: Cashmere, Sheep down, Mitochondria DNA, Cyt b gene, Gene chip

吕雪峰,阿布力孜?阿布力米提,帕娜尔,陶卫东,邢巍婷,采复拉,郑文新. 利用基因芯片技术鉴别山羊绒和绵羊绒[J]. 生物技术通报, 2014, 0(1): 196-200.

Lü Xuefeng, Abliz Ablimit Pa Naer, Tao Weidong, Xing Weiting, Cai Fula, Zheng Wenxin. Identification of Cashmere and Sheep down by Microarray Technology[J]. Biotechnology Bulletin, 2014, 0(1): 196-200.

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利用基因芯片技术鉴别山羊绒和绵羊绒

Identification of Cashmere and Sheep down by Microarray Technology

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