猪圆环病毒2型三株贵州流行株全基因序列分析

生物技术通报 ›› 2014, Vol. 0 ›› Issue (3): 165-170.

猪圆环病毒2型三株贵州流行株全基因序列分析

李涛¹, 王开功^2,3, 程振涛^2,3, 周碧君^2,3, 文明^2,3, 崔亚兰³

(1.贵州省农业委员会，贵阳 550001；2.贵州省生猪质量安全工程技术研究中心，贵阳 550001；3.贵州大学动物科学学院，贵阳 550025)

收稿日期:2013-09-16 出版日期:2014-03-29 发布日期:2014-03-31
作者简介:李涛，男，研究方向:动物传染病与综合防控；E -mail:727670509@qq.com
基金资助:
贵州省生猪质量安全工程技术研究中心建设项目[黔科合农C字(2011)4022号]，贵州省农业委员会科技计划项(2013-06号)

Complete Gene Sequencing and Genetic Analysis of PCV2 Isolates from Guizhou Province

Li Tao¹, Wang Kaigong^2,3, Cheng Zhentao^2,3, Zhou Bijun^2,3, Wen Ming^2,3 , Cui Yalan³

(1. The Agricultrual Committee of Guizhou Province，Guiyang 550001；2.Guizhou Animal Disease Control Center，Guiyang 550001；3. College of Animal Science，Guizhou University，Guiyang 550025)

Received:2013-09-16 Published:2014-03-29 Online:2014-03-31

摘要/Abstract

摘要： 为掌握贵州省猪圆环病毒2型(PCV2)流行株的分子生物学资料，对已分离的3株PCV2贵州流行株(GZ-LZ1株、GZ-KY1株和GZ-PX1株)进行全基因克隆与测序，应用生物信息学软件将贵州PCV2流行株与参考株进行核苷酸同源性与系统进化树分析。结果显示，3株PCV2贵州流行株贵州全基因组大小均为1 767 bp，同源性为97.0%-98.3%，与国内外24株参考株同源性为77.5%-99.9%，与国产疫苗株同源性为95.5%-98.2%；系统进化树结果显示，3株PCV2贵州流行株归属PCV2d分支，其中GZ-LZ1株和GZ-PX1株与国内PCV2疫苗YM-SH株的进化关系较远，而GZ-KY1与四川SC-10株、浙江ZJ-38株和黑龙江HLJ-10株的进化关系较近。

关键词: 猪圆环病毒2型, 全基因序列, 分离株的遗传分析

Abstract: It was to get the molecular biology data of the PCV2 strains in Guizhou province, 3 isolations of PCV2(GZ-LZ1strain, GZ-KY1strain and GZ-PX1 strain)were cloned and sequenced. The sequences were then multiple-aligned with representative sequences published in GenBank, and phylogenetic analysis was performed, both using bioinfromatics software. Sequence analysis showed that complete gene of all the 3 PCV2 isolates were 1 767 bp, the accession numbers were, KC788503, KC788504and KC788505. In pairwise comparisons, they were closely relate to each other and to the strains of vaccine producted in China with high nucleotide sequence identity from 97.0% to 98.3% and 95.5%-98.2%, respectively. However, they were more divergent from those of other countries or orther regions of China with lower level of similarities from 77.5% to 99.9%. GZ-LZ1 and GZ-PX1 were closely relate to the the strainYM-SH of vaccine, the strain of GZ-KY1 Phylogenetic analysis revealed that all of the 3 isolates were in the branch of PCV2d, strains of was closely relate to the SC-10 strain, ZJ-38 strain and HLJ-10.

Key words: PCV2, Complete gene sequencing, Genetic analysis of isolates

李涛, 王开功, 程振涛, 周碧君, 文明, 崔亚兰. 猪圆环病毒2型三株贵州流行株全基因序列分析[J]. 生物技术通报, 2014, 0(3): 165-170.

Li Tao, Wang Kaigong, Cheng Zhentao, Zhou Bijun, Wen Ming , Cui Yalan. Complete Gene Sequencing and Genetic Analysis of PCV2 Isolates from Guizhou Province[J]. Biotechnology Bulletin, 2014, 0(3): 165-170.

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