基因组转录调控元件分析方法研究进展

摘要/Abstract

摘要： 真核生物的基因表达是一个非常复杂的过程,需要多种转录调控元件的协同作用精确调控。全基因组转录调控元件的筛选和鉴定对于基因表达调控机制和生物学功能研究具有重要意义。但转录调控元件的高效筛选及功能验证仍是研究人员面临的主要挑战。结合近年来转录调控元件的研究成果,对基因组中转录调控元件的预测、筛选及功能验证方法做一综述。

关键词: 转录调控元件, 高通量筛选, 标志物

Abstract: Expression of eukaryotic genes is a complex process, achieved through the coordinated action of transcription regulatory elements. The screening and identification of transcriptional regulatory elements in genome scale have crucial significance for understanding the mechanism and biological function of gene expression regulation. However, the effectively screening and function verification is still the major challenges for researchers. Herein, based on the latest research findings, we reviewed the methods of prediction, screening, function analysis of transcription regulatory elements in genome.

Key words: Transcriptional regulatory element, High throughput screening, Biomark

李文茂, 贾东方, 许嵘. 基因组转录调控元件分析方法研究进展[J]. 生物技术通报, 2014, 0(10): 64-70.

Li Wenmao, Jia Dongfang, Xu Rong. Research Advance in Genome-wide Identification of Transcription Regulatory Elements[J]. Biotechnology Bulletin, 2014, 0(10): 64-70.

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