细胞衰老相关基因MTMR2功能初探及相互作用蛋白的鉴定

摘要/Abstract

摘要： 过氧化氢（H₂O₂）诱导的细胞衰老在肿瘤形成中有重要作用,为进一步研究其内在机制,通过全基因组shRNA文库筛选出与之相关的MTMR2（Myotubularin-related protein 2）基因,并通过β-半乳糖苷酶染色试验证实其在H₂O₂诱导的细胞衰老中发挥重要作用。通过克隆质粒、表达纯化并结合质谱分析,发现了MTMR2的一个可能的重要相互作用蛋白14-3-3,并通过生化方法对两者的相互作用进行确认。

关键词: 过氧化氢, 细胞衰老, MTMR2, shRNA文库筛选, shRNA

Abstract: H₂O₂-induced cell senescence plays an important role in tumorigenesis. To further study the underlying mechanism, we identified five relevant members through a genome wide shRNA screening and took MTMR2（Myotubularin-related protein 2） as an example to figure out its function in H₂O₂-induced cell senescence. By plasmid construction and expression in combination with purification and mass spectrometry, we found a significant interacting protein of MTMR2：14-3-3 proteins family, and their interaction was then confirmed using biochemical methods.

Key words: H₂O₂, Cell senescence, MTMR2 , shRNA pool screening , shRNA

李超群,陈亚丽,肖冬光,裴华东. 细胞衰老相关基因MTMR2功能初探及相互作用蛋白的鉴定[J]. 生物技术通报, 2014, 0(11): 201-205.

Li Chaoqun,Chen Yali,Xiao Dongguang,Pei Huadong. MTMR2 Interacts with 14-3-3 and Regulates H₂O₂ Induced Cell Senescence[J]. Biotechnology Bulletin, 2014, 0(11): 201-205.

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