生物技术通报 ›› 2014, Vol. 0 ›› Issue (12): 121-127.doi: 10.13560/j.cnki.biotech.bull.1985.2014.12.020

• 研究报告 • 上一篇    下一篇

三种植物乙醇粗提物对松梢螟幼虫的生物活性研究

周洁尘, 闫瑞坤 ,王圣洁 ,何苑皞, 谭益明 ,周国英   

  1. 中南林业科技大学 经济林培育与保护省部共建教育部重点实验室,长沙 410004
  • 收稿日期:2014-04-21 出版日期:2014-12-08 发布日期:2014-12-12
  • 作者简介:周洁尘,女,硕士研究生,研究方向:病虫害无公害防治技术;E-mail:244756681@qq.com
  • 基金资助:
    林业公益性行业科研专项经费项目(201004014)

Bioactivities of the Alcohol Extracts from Three Plants Against Dioryctria splendidella Larvaes

Zhou Jiechen Yan Ruikun Wang Shengjie He Yuanhao Tan Yiming Zhou Guoying   

  1. (Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees(Central South University of Forestry and Technology), Ministry of Education, Changsha 410004)
  • Received:2014-04-21 Published:2014-12-08 Online:2014-12-12

摘要: 在室内测定了垂序商陆全草、柚果皮、博落回全草乙醇粗提物对松梢螟幼虫的生物活性,并测定了其林间防效。 结果显示,不同浓度 3 种植物乙醇粗提物对松梢螟幼虫都有一定的生物活性,且浓度越高作用效果越强。柚皮粗提物对松梢螟幼 虫的生物活性最强,100 mg/mL 的柚皮粗提物对松梢螟幼虫的最高毒杀校正死亡率、拒食率、生长抑制率分别为 80.00%、99.28% 和 96.32%,同时其林间防治效果为 55.70%,与对照药剂噻虫啉的防治效果在同一显著水平。垂序商陆粗提物对松梢螟幼虫的生物 活性最弱,但其高浓度粗提物校正死亡率仍在 70% 以上,拒食率以及生长抑制率也在 90% 以上。

关键词: 乙醇粗提物, 松梢螟, 毒杀作用, 拒食作用, 生长抑制作用

Abstract: In order to study the bioactivities of ethanol extracts from Phytolacca americana, Citrus maxima peels and Macleaya cordata against the larvaes of Dioryctria splendidella preliminarily in laboratory, contact toxicity, antifeedant activities and body weight gains of D. splendidella larvaes were determined after treated with 3 ethanol extracts. The results indicated that 3 ethanol extracts with different concentration had some bioactivities against the larvae of D. splendidella, and the higher the extracted concentrations, the more obvious of the bioactivities to the larvaes were. When the concentration of Citrus maxima peels extracts was 100 mg/mL, there were the strongest bioactivities. The contact toxicity corrected mortality, antifeedant rate and growth inhibiting rate reached to 80.00%, 99.28%, and 96.32%, respectively. The control effect in the forest was 55.70%, the same as the pesticides thiacloprid. The ethanol extracts from Phytolacca americana had the lowest bioactivities, but the corrected mortality of ethanol extracts being high concentration were more than 70%, antifeedant rates and growth inhibiting rates were more than 90%.

Key words: Ethanol extracts, Dioryctria splendidella, Contact toxicity, Antifeedant activities, Growth inhibition