液态烷烃氧载体对粘红酵母发酵产番茄红素的影响

doi:10.13560/j.cnki.biotech.bull.1985.2015.02.029

生物技术通报 ›› 2015, Vol. 31 ›› Issue (2): 196-201.doi: 10.13560/j.cnki.biotech.bull.1985.2015.02.029

液态烷烃氧载体对粘红酵母发酵产番茄红素的影响

李娜娜, 吴晓英, 吴振强

华南理工大学生物科学与工程学院，广州 510006

收稿日期:2014-08-19 出版日期:2015-02-05 发布日期:2015-02-06
作者简介:李娜娜，女，硕士研究生，研究方向：生物活性物质的研究；E-mail：759365635@qq.com

A Effect of Liquid Alkane Oxygen-vectors on Rhodotorula Fermentations and Lycopene Production

Li Nana, Wu Xiaoying, Wu Zhenqiang

School of Bioscience＆Bioengineering, South China University of Techology, Guangzhou 510006

Received:2014-08-19 Published:2015-02-05 Online:2015-02-06

摘要/Abstract

摘要： 研究正己烷、正十二烷、正十六烷3种液态烷烃作为氧载体对粘红酵母生长和番茄红素合成的影响，发现氧载体不仅使菌体生物量提高，同时使单位细胞的番茄红素产率增大，从而提高粘红酵母合成番茄红素的能力。3种液态烷烃中正十二烷作为氧载体效果较好，试验结果表明，在发酵第0 h时添加4%的正十二烷，细胞生物量达到16.49 g/L，番茄红素合成量达到42.32 mg/L分别比对照组提高了26.2%和50.17%。

关键词: 粘红酵母, 番茄红素, 液态烷烃, 氧载体, 传递效率

Abstract: Addition of oxygen-vectors（n-hexane, n-dodecane, hexadecane）to fermentation medium was recognized as a method of enhancing oxygen transfer and promoting lycopene yield by Rhodotorula fermentation. n-dodecane as an oxygen-vector is the best in three kinds of liquid alkane. Experimental results show that the biomass of Rhodotorula attained 16.49 g/L and the yield of lycopene biosynthetic reached 42.32 mg/L by adding 4% n-dodecane in the 0 h, which was 26.2% and 50.17% higher than the control group, respectively.

Key words: Rhodotorula, lycopene, liquid alkane, oxygen-vectors, oxygen transfer

李娜娜, 吴晓英, 吴振强. 液态烷烃氧载体对粘红酵母发酵产番茄红素的影响[J]. 生物技术通报, 2015, 31(2): 196-201.

Li Nana, Wu Xiaoying, Wu Zhenqiang. A Effect of Liquid Alkane Oxygen-vectors on Rhodotorula Fermentations and Lycopene Production[J]. Biotechnology Bulletin, 2015, 31(2): 196-201.

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液态烷烃氧载体对粘红酵母发酵产番茄红素的影响

A Effect of Liquid Alkane Oxygen-vectors on Rhodotorula Fermentations and Lycopene Production

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