樟芝挥发油的微波辅助双液相萃取及其抗菌活性研究

doi:10.13560/j.cnki.biotech.bull.1985.2015.12.018

生物技术通报 ›› 2015, Vol. 31 ›› Issue (12): 122-130.doi: 10.13560/j.cnki.biotech.bull.1985.2015.12.018

樟芝挥发油的微波辅助双液相萃取及其抗菌活性研究

刘琳¹,陈慧黠²,郭立忠¹

1. 青岛农业大学生命科学学院山东省应用真菌重点实验室，青岛 266109；2. 大连海洋大学水产与生命学院，大连 116023

收稿日期:2015-03-28 出版日期:2015-12-19 发布日期:2015-12-19
作者简介:刘琳，女，博士，讲师，研究方向:微生物生物转化与分离；E-mail:liulin@qau.edu.cn
基金资助:
国家自然科学基金项目(31172012/C1506)，青岛农业大学高层次人才科研基金项目(6631112308)，辽宁省教育厅计划项目项目(L2012255)

The Microwave-assisted Two-phase Solvent Extraction of Essential Oil from Taiwanofungus camphoratus and Its Antifungal Activity

Liu Lin¹, Chen Huixia², Guo Lizhong¹

1. College of Life Science，Qingdao Agricultural University，Shandong Provincial Key Laboratory of Applied Mycology，Qingdao 266109；2. College of Life Science, Dalian Ocean University，Dalian 116023

Received:2015-03-28 Published:2015-12-19 Online:2015-12-19

摘要/Abstract

摘要： 采用微波辅助石油醚/乙醇双液相萃取技术提取樟芝发酵液中的挥发油，并考察了其对7种皮肤癣菌的最低抑制浓度(MIC)。优化的提取条件为:乙醇体积浓度56%，石油醚体积浓度30%，固液比1:50，微波功率380 W，微波时间90 s。此时，挥发油的提取率为0.69%，真菌MIC为5-20 mL/L。微波辅助双液相萃取挥发油耗时短，提取率高，且获得的挥发油抗皮肤癣菌效果显著。

关键词: 微波辅助双液相萃取, 挥发油, 樟芝, 最低抑制浓度(MIC), 皮肤癣菌

Abstract: In this study microwave-assisted petroleum ether/ethanol two-phase solvent extraction was applied to extract the essential oil from the fermentation broth of Taiwanofungus camphoratus, and the minimal inhibitory concentration(MIC)of the essential oil against 7 species of dermatophyte were determined. The optimized extraction conditions are as following:ethanol concentration 56%(v/v), petroleum ether concentration 30%(v/v), solid-to-liquid ratio 1:50, microwave power 380 W and microwave time 90 s. The highest yield of extracted essential oil was 0.69%, and the MIC against the tested fungi were 5-20 mL/L. This extraction technology is efficient and time saving, moreover, the produced essential oil shows significant inhibitory effect on the growth of the tested dermatophyte.

Key words: microwave-assisted two-phase solvent extraction, essential oil, Taiwanofungus camphoratus, minimal inhibitory concentration(MIC), dermatophyte

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刘琳,陈慧黠,郭立忠. 樟芝挥发油的微波辅助双液相萃取及其抗菌活性研究[J]. 生物技术通报, 2015, 31(12): 122-130.

Liu Lin, Chen Huixia, Guo Lizhong. The Microwave-assisted Two-phase Solvent Extraction of Essential Oil from Taiwanofungus camphoratus and Its Antifungal Activity[J]. Biotechnology Bulletin, 2015, 31(12): 122-130.

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樟芝挥发油的微波辅助双液相萃取及其抗菌活性研究

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[1]	郑焕, 林冬梅, 刘峻源, 张引莲, 林标声, 林占熺, 李晶. 基于LC-QTOF-MS代谢组学解析牛樟芝子实体和菌丝体中氨基酸代谢差异[J]. 生物技术通报, 2023, 39(5): 254-266.
[2]	李晶, 冯娜, 王升陽, 林占熺. 牛樟芝化学成分及其药理作用研究进展[J]. 生物技术通报, 2021, 37(11): 14-31.
[3]	严宽, 陈放, 魏琴, 冯瑞章, 周万海, 周敏. 内生真菌对油樟挥发油积累及生理生化特性的影响[J]. 生物技术通报, 2017, 33(3): 138-143.
[4]	王珊珊, 吴昊, 李梁. 花椒挥发油的提取及其抑菌抗虫活性测定[J]. 生物技术通报, 2017, 33(11): 101-105.
[5]	. 山香挥发油的化学组成和杀虫活性[J]. , 2004, 0(03): 58-58.
[6]	李思经;. 微繁殖薄荷、果树和欧洲越桔的田间试验[J]. , 1990, 0(06): 16-17.