饲喂Bt水稻的褐家鼠肠道中cry1Ab/Ac基因和蛋白的残留检测

doi:10.13560/j.cnki.biotech.bull.1985.2015.12.019

生物技术通报 ›› 2015, Vol. 31 ›› Issue (12): 131-137.doi: 10.13560/j.cnki.biotech.bull.1985.2015.12.019

饲喂Bt水稻的褐家鼠肠道中cry1Ab/Ac基因和蛋白的残留检测

徐灯^1,2,耿丽丽²,李宁²,刘晓辉²,卢凡¹,张杰²

1.湖北工业大学资源与环境学院，武汉 450068；
2.中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室，北京 100193

收稿日期:2015-04-26 出版日期:2015-12-19 发布日期:2015-12-19
作者简介:徐灯，男，硕士，研究方向:转基因生物安全评价；E-mail:xudg1230@163.com
基金资助:
转基因生物新品种培育重大专项(2012ZX08011001)

The Residue Detection of cry1Ab/Ac Gene and Protein in the Gut of Rattus norvegicus Feeding Transgenic Rice

Xu Deng^1,2, Geng Lili², Li Ning², Liu Xiaohui², Lu Fan¹, Zhang Jie²

1. School of Resource & Environmental Engineering，Hubei University of Technology，Wuhan 450068；
2. State Key Laboratory for Biology of Plant Diseases and Insect Pests，Institute of Plant Protection，Chinese Academy of Agricultural Sciences，Beijing 100193

Received:2015-04-26 Published:2015-12-19 Online:2015-12-19

摘要/Abstract

摘要：

分别用含有转cry1Ab/Ac基因水稻和亲本水稻的饲料饲喂野生褐家鼠，旨在检测外源cry1Ab/Ac基因和蛋白在褐家鼠雌鼠雄鼠空肠、回肠、盲肠中的残留情况。检测结果显示，饲喂转基因水稻50 d和110 d的雌雄褐家鼠肠道3个部位中没有检测到cry1Ab/Ac基因残留，在体外消化实验中过量外源基因全长及片段在4 h后开始逐步降解，约12 h全部降解。3个肠道部位提取的蛋白进行ELISA和Western blot分析，结果显示肠道内容物中无Cry1Ab/Ac蛋白残留。因此外源基因和蛋白不会在饲喂了转基因水稻的野生褐家鼠肠道中残留。

关键词: 转基因水稻, 褐家鼠, 基因、蛋白残留, 体外消化

Abstract:

The exogenous cry1Ab/Ac gene and protein residues in the jejunum, ileum, cecum of Rattus norvegicus feeding transgenic rice and parent rice were detected. The testing results showed that there were no residues of cry1Ab/Ac gene in 3 intestinal parts of R. norvegicus fed transgenic rice for 50 and 110 d, and overall length or fragment of exogenous cry1Ab/Ac gene gradually degraded after 4 h, and completely at 12 h in vitro. No residues of Cry1Ab/Ac protein were detected in contents of the above 3 parts of R. norvegicus using ELISA and Western blot method. In conclusion, the exogenous gene and protein of the transgenic rice were not remained in the intestines of R. norvegicus.

Key words: transgenic rice, Rattus norvegicus, gene and protein residues, digestion in vitro

徐灯,耿丽丽,李宁,刘晓辉,卢凡,张杰. 饲喂Bt水稻的褐家鼠肠道中cry1Ab/Ac基因和蛋白的残留检测[J]. 生物技术通报, 2015, 31(12): 131-137.

Xu Deng, Geng Lili, Li Ning, Liu Xiaohui, Lu Fan, Zhang Jie. The Residue Detection of cry1Ab/Ac Gene and Protein in the Gut of Rattus norvegicus Feeding Transgenic Rice[J]. Biotechnology Bulletin, 2015, 31(12): 131-137.

参考文献

[1] James C. Global status of commercialized biotech/GM crops:2014[J]. ISAAA Brief 47, 2015.
[2] 姜玉英, 曾娟, 陆明红. 2015年全国三大谷类作物重大病虫害发生趋势预报[J]. 植物保护, 2015(2):1-4.
[3] Tu JM, Zhang GA, Datta K, et al. Field performance of transgenic elite commercial hybrid rice expressing Bacillus thuringiensis delta-endotoxin[J]. Nature Biotechnology, 2000, 18(10):1101-1104.
[4] Lu C. The first approved transgenic rice in China[J]. GM Crops, 2010, 1(3):113-115.
[5] 江佳富, 吴晓明, 左曙青. 北京市褐家鼠种群遗传多态性及与汉坦病毒分布关系的研究[J]. 中国媒介生物学及控制杂志, 2005(5):30-35.
[6] Edwards GR, Crawley MJ. Rodent seed predation and seedling recruitment in mesic grassland[J]. Oecologia, 1999, 118(3):288-296.
[7] Pearson DE, Hierro JL, Chiuffo M, et al. Rodent seed predation as a biotic filter influencing exotic plant abundance and distribution[J]. Biological Invasions, 2014, 16(5):1185-1196.
[8] Wang ZH, Wang Y, Cui HR. Toxicological evaluation of transgenic rice flour with a synthetic crylAb gene from Bacillus thuringiensis[J]. Journal of the Science of Food and Agriculture, 2002, 82(7):738-744.
[9] Schr?der M, Poulsen M, Wilcks A, et al. A 90-day safety study of genetically modified rice expressing Cry1Ab protein(Bacillus thuringiensis toxin)in Wistar rats[J]. Food and Chemical Toxicology, 2007, 45(3):339-349.
[10] Song H, He X, Zou S, et al. A 90-day subchronic feeding study of genetically modified rice expressing Cry1Ab protein in Sprague-Dawley rats[J]. Transgenic Research, 2014:1-14.
[11] Kroghsbo S, Madsen C, Poulsen M, et al. Immunotoxicological studies of genetically modified rice expressing PHA-E lectin or Bt toxin in Wistar rats[J]. Toxicology, 2008, 245(1-2):24-34.
[12] Cao SS, He XY, Xu WT, et al. Safety assessment of Cry1C protein from genetically modified rice according to the national standards of PR China for a new food resource[J]. Regulatory Toxicology and Pharmacology, 2010, 58(3):474-481.
[13] Ding JY, Jia JW, Yang LT, et al. Validation of a rice specific gene, sucrose phosphate synthase, used as the endogenous reference gene for qualitative and real-time quantitative PCR detection of transgenes[J]. Journal of Agricultural and Food Chemistry, 2004, 52(11):3372-3377.
[14] Jeong SC, Pack IS, Cho EY, et al. Molecular analysis and quantitative detection of a transgenic rice line expressing a bifunctional fusion TPSP[J]. Food Control, 2007, 18(11):1434-1442.
[15] 徐灯, 耿丽丽, 张敏红. 转基因水稻cry1Ab/Ac基因及蛋白在肉鸡肠道中的残留检测[J]. 生物技术通报, 2015, 31(9):84-90.
[16] Martin-Orue SM, O'Donnell AG, Arino J, et al. Degradation of transgenic DNA from genetically modified soya and maize in human intestinal simulations[J]. British Journal of Nutrition, 2002, 87(6):533-542.
[17] Mills ENC, Madsen C, Shewry PR, et al. Food allergens of plant origin-their molecular and evolutionary relationships[J]. Trends in Food Science & Technology, 2003, 14(4):145-156.
[18] Tudisco R, Lombardi P, Bovera F, et al. Genetically modified soya bean in rabbit feeding:detection of DNA fragments and evaluation of metabolic effects by enzymatic analysis[J]. Animal Science, 2006, 82:193-199.
[19] Chowdhury EH, Shimada N, Murata H, et al. Detection of Cry1Ab protein in gastrointestinal contents but not visceral organs of genetically modified Bt11-fed calves[J]. Vet Hum Toxicol, 2003, 45(2):72-75.
[20] Einspanier R, Lutz B, Rief S, et al. Tracing residual recombinant feed molecules during digestion and rumen bacterial diversity in cattle fed transgene maize[J]. European Food Research and Technology, 2004, 218(3):269-273.
[21] Lutz B, Wiedemann S, Einspanier R, et al. Degradation of Cry1Ab protein from genetically modified maize in the bovine gastrointestinal tract[J]. J Agric Food Chem, 2005, 53(5):1453-1456.
[22] Yuan Y, Xu W, He X, et al. Effects of genetically modified T2A-1 rice on the GI health of rats after 90-day supplement[J]. Scientific Reports, 2013, 3(1962):srep01962.
[23] Swiatkiewicz M, Bednarek D, Markowski J, et al. Effect of feeding genetically modified maize and soybean meal to sows on their reproductive traits, haematological indices and offspring performance[J]. Bulletin of the Veterinary Institute in Pulawy, 2013, 57(3):413-418.
[24] Walsh MC, Buzoianu SG, Gardiner GE, et al. Fate of transgenic DNA from orally administered Bt MON810 maize and effects on immune response and growth in pigs[J]. PLoS One, 2011, 6(11):12.
[25] Furgal-Dierzuk I, Strzetelski J, Kwiatek K, et al. The effect of genetically modified maize(MON 810)and soyabean meal(Roundup Ready)on rearing performance and transfer of transgenic DNA to calf tissues[J]. Journal of Animal and Feed Sciences, 2014, 23(1):13-22.
[26] Jennings JC, Kolwyck DC, Kays SB, et al. Determining whether transgenic and endogenous plant DNA and transgenic protein are detectable in muscle from swine fed Roundup Ready soybean meal[J]. Journal of Animal Science, 2003, 81(6):1447-1455.

饲喂Bt水稻的褐家鼠肠道中cry1Ab/Ac基因和蛋白的残留检测

The Residue Detection of cry1Ab/Ac Gene and Protein in the Gut of Rattus norvegicus Feeding Transgenic Rice

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