基于氨基酸序列和模拟结构预测蛋白质稳定性的研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2017.04.011

摘要/Abstract

摘要： 稳定性是蛋白质重要性质之一,工业用蛋白质需要良好的稳定性,进化过程中蛋白质新功能的衍生也依赖于蛋白质的稳定性。提高蛋白质的稳定性,尤其是提高未知结构蛋白质的稳定性是一项非常具有挑战性的工作——传统的蛋白质改造方法如定向进化费时费力,传统的理性设计则难以被其他研究者复制。虽然目前有很多蛋白质稳定性预测工具,但这些预测工具大多都需要预先测定蛋白质的三维结构,因此结构未知的蛋白质的稳定性预测受到了限制。近年来,人们开发了一些利用蛋白质的氨基酸序列和模拟结构来预测突变对结构未知蛋白质稳定性影响的预测工具。介绍该方面的研究进展,以期为蛋白质工程研究提供参考。

关键词: 蛋白质稳定性, 预测工具, 氨基酸序列, 蛋白质结构模拟

Abstract: Stability is one of the important properties of protein. Protein’s stability is not only demanded in industry,but also the prerequisite for its deriving new functions in protein evolution. Improving the stability of protein,especially protein of unknown structure,is a very challenging task,i.e.,traditional protein modification methods such as directed evolution are time-consuming and laborious,while rational design is difficult to replicate by other researchers. At present,there are many reported predictors for protein stability. However,the majority of these tools require the pre-measurement of three-dimensional structure of protein,which restricts the stability prediction of protein in unknown structure. Recently,a number of tools to predict the effects of mutations on the stability of unknown proteins have been developed using amino acid sequences and simulated structures of proteins,here we introduce the research progress on them,aiming at providing guidance for protein engineering.

Key words: protein stability, predictors, amino acid sequence, protein structure simulation

易华伟唐晓峰. 基于氨基酸序列和模拟结构预测蛋白质稳定性的研究进展[J]. 生物技术通报, 2017, 33(4): 83-89.

YI Hua-Wei, TANG Xiao-Feng. Research Progress on the Prediction of Protein Stability Based on Amino Acid Sequence and Simulated Structure[J]. Biotechnology Bulletin, 2017, 33(4): 83-89.

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