基于同位素标记定量分析烟碱诱导SH-SY5Y细胞的差异表达蛋白

doi:10.13560/j.cnki.biotech.bull.1985.2017.04.012

生物技术通报 ›› 2017, Vol. 33 ›› Issue (4): 90-97.doi: 10.13560/j.cnki.biotech.bull.1985.2017.04.012

基于同位素标记定量分析烟碱诱导SH-SY5Y细胞的差异表达蛋白

朱贝贝, 李翔宇, 陈欢, 王红娟, 侯宏卫, 胡清源

国家烟草质量监督检验中心,郑州 450001

收稿日期:2016-10-19 出版日期:2017-04-25 发布日期:2017-04-25
作者简介:朱贝贝,女,硕士研究生,研究方向：吸烟与健康及生物标志物;E-mail：beibeizhus@126.com
基金资助:
中国烟草总公司科技重点项目（110201402037）

iTRAQ-based Quantitative Proteomic Analyses of Differentially Expressed Proteins in Nicotine-induced SH-SY5Y Cells

ZHU Bei-bei, LI Xiang-yu, CHEN Huan, WANG Hong-juan, HOU Hong-wei, HU Qing-yuan

China National Tobacco Quality Supervision & Test Centre,Zhengzhou 450001

Received:2016-10-19 Published:2017-04-25 Online:2017-04-25

摘要/Abstract

摘要： 以烟碱诱导后的人神经母瘤细胞株为材料,分析烟碱处理后细胞蛋白表达谱的变化,应用iTRAQ标记结合nano-LC-Q-TOF MS技术,鉴定烟碱诱导神经元细胞的差异表达蛋白质并对其进行生物信息学分析。结果显示,烟碱处理组与对照组相比共有132个蛋白差异表达（|ratio|≥1.5,P<0.05）,差异表达的蛋白主要参与基因表达、MAPK级联反应、GTP调节信号传导和神经营养因子TRK受体信号传导等生物学过程。其中烟碱可能通过改变蛋白酶体调节神经细胞的平衡性而产生烟碱依赖,MAPK级联反应和泛素化-蛋白酶体通路在这一过程中发挥重要作用。

关键词: 差异蛋白组学, iTRAQ, 烟碱, SH-SY5Y

Abstract: SH-SY5Y,induced by nicotine,was used to seek the effects of nicotine exposure on the alteration of proteins expression profile. The differentially expressed proteins in nicotine-induced neuronal cells were identified using the iTRAQ and nano-LC-Q-TOF MS proteomics,and their bioinformatics was also analyzed. The results showed that the levels of 132 proteins were differentially altered in response to nicotine（| ratio | ≥ 1.5,P<0.05）,including proteins involved in gene expression,MAPK cascade,GTP signal transduction and neurotrophin TRK receptor signaling. Together,our data suggested that the nicotine might elicit dependence by altering the neuronal balance of proteasome subunits,with the MAPK cascade and ubiquitin-proteasome pathway playing a pivotal role.

Key words: differential proteomics, iTRAQ, nicotine, neuronal SH-SY5Y

朱贝贝, 李翔宇, 陈欢, 王红娟, 侯宏卫, 胡清源. 基于同位素标记定量分析烟碱诱导SH-SY5Y细胞的差异表达蛋白[J]. 生物技术通报, 2017, 33(4): 90-97.

ZHU Bei-bei, LI Xiang-yu, CHEN Huan, WANG Hong-juan, HOU Hong-wei, HU Qing-yuan. iTRAQ-based Quantitative Proteomic Analyses of Differentially Expressed Proteins in Nicotine-induced SH-SY5Y Cells[J]. Biotechnology Bulletin, 2017, 33(4): 90-97.

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基于同位素标记定量分析烟碱诱导SH-SY5Y细胞的差异表达蛋白

iTRAQ-based Quantitative Proteomic Analyses of Differentially Expressed Proteins in Nicotine-induced SH-SY5Y Cells

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