长链非编码RNA AK043773在脂肪细胞分化中的作用

doi:10.13560/j.cnki.biotech.bull.1985.2017-0556

生物技术通报 ›› 2017, Vol. 33 ›› Issue (12): 156-161.doi: 10.13560/j.cnki.biotech.bull.1985.2017-0556

长链非编码RNA AK043773在脂肪细胞分化中的作用

张娟娟, 乔玲, 朱恩东

天津医科大学代谢病医院内分泌研究所卫生部激素与发育重点实验室天津市代谢性疾病重点实验室，天津 300070

收稿日期:2017-07-04 出版日期:2017-12-25 发布日期:2017-12-21
作者简介:张娟娟，硕士研究生，研究方向：脂肪与成骨细胞分化调控研究；E-mail：839973071@qq.com
基金资助:
国家自然科学基金项目（81501846），天津医科大学代谢病医院内分泌研究所科学基金（2014RC01）

Role of Long Non-coding RNA AK043773 in Adipocyte Differentiation

ZHANG Juan-juan, QIAO Ling, ZHU En-dong

Key Laboratory of Hormones and DevelopmentMinistry of Health，Tianjin Key Laboratory of Metabolic Diseases，Metabolic Diseases Hospital & Institute of Endocrinology，Tianjin Medical University，Tianjin 300070

Received:2017-07-04 Published:2017-12-25 Online:2017-12-21

摘要/Abstract

摘要： 探索长链非编码RNA（lncRNA）AK043773在脂肪细胞分化过程中的表达变化和调控作用。将小鼠前脂肪细胞成脂诱导，利用实时定量PCR技术检测成脂分化调控基因KLF7及AK043773的表达，构建AK043773过表达载体检测其对成脂分化的影响。经UCSC数据库检索发现AK043773位于KLF7基因4号外显子的3'端下游，骨髓基质细胞系ST2和前脂肪细胞系3T3-L1成脂诱导分化后，实时定量PCR检测结果显示AK043773及KLF7表达协同下调；构建pCDNA3.1-AK043773过表达载体，转染ST2细胞后能显著提高AK043773的表达，进行脂肪细胞分化条件诱导，通过油红O染色及OD520nm吸光值检测可见AK043773过表达显著抑制ST2细胞中脂滴产生。AK043773及KLF7在脂肪细胞分化过程中协同下调，上调AK043773的表达可显著抑制脂肪细胞分化。

关键词: 长链非编码RNA, AK043773, 脂肪细胞分化, KLF7

Abstract: This work is to investigate the expression changes and regulating role of long non-coding RNA（lncRNA）AK043773 in adipocyte differentiation. First，pre-adipocyte cells in mice were induced for adipogenesis. Then，the expressions of adipogenic differentiation regulating gene（KLF7）and AK043773 were measured by real-time quantitative PCR（RT-qPCR）. Next，the overexpression vector for AK043773 was constructed for detecting its effects on adipogenic differentiation. According to search in UCSC database，the locus of AK043773 was in the 3'-end downstream of KLF7’s exon 4. The expressions of KLF7 and AK043773 were down-regulated coordinately in adipogenesis-induced and differentiated bone marrow stromal cell line ST2 and pre-adipocyte cells 3T3-L1. Furthermore，the overexpression vector pCDNA3.1-AK043773 was constructed and transfected into ST2 cells，which significantly enhanced the expression of AK043773，but inhibited the adipogenesis in ST2 cells by oil red O staining and OD520nm absorbance test after the induction of adipocyte differentiation. All of results indicate that AK043773 and KLF7 coordinately down-regulate in adipocyte differentiation，and increasing the expression of AK043773 remarkably suppresses the differentiation of adipocyte.

Key words: lncRNA, AK043773, adipocyte differentiation, KLF7

张娟娟, 乔玲, 朱恩东. 长链非编码RNA AK043773在脂肪细胞分化中的作用[J]. 生物技术通报, 2017, 33(12): 156-161.

ZHANG Juan-juan, QIAO Ling, ZHU En-dong. Role of Long Non-coding RNA AK043773 in Adipocyte Differentiation[J]. Biotechnology Bulletin, 2017, 33(12): 156-161.

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长链非编码RNA AK043773在脂肪细胞分化中的作用

Role of Long Non-coding RNA AK043773 in Adipocyte Differentiation

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