植物长链非编码RNA调控发育与胁迫应答的研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2022-0053

摘要/Abstract

摘要：

长链非编码RNAs（long non-coding RNAs，lncRNAs）是一类长度大于200个核苷酸、缺乏明显开放阅读框、很少或者不具有蛋白编码潜能的内源性RNA。鉴于lncRNAs低表达和低保守性的特点，早期阶段认为其是转录副产物，在生物体内不发挥生物学功能。随着对非编码RNA的深入研究，lncRNAs被认为是一种调控其他类型RNA的重要基因组分，参与发育和胁迫应答生物学过程。本文主要阐述lncRNAs的来源及分类、作用机制、lncRNAs在植物发育和胁迫应答方面的生物学功能，为lncRNAs在作物生产育种中的应用研究提供参考。

关键词: 长链非编码RNAs, 植物, 发育, 胁迫应答

Abstract:

Long non-coding RNAs（lncRNAs）are a class of endogenous RNAs that are longer than 200 nucleotides, and lack apparent protein-coding capacity without open reading frame. Due to the low expression level and weak conservation, lncRNAs are considered to be transcription by-product, which play no biological functions in vivo. With the further research on non-coding RNAs, they are confirmed to be an important component regulating other type RNAs, which are involved in development and stress response biological processes. In this review, we summarize the generation and classification, mechanism, the biological functions of lncRNAs in development and stress response, aiming to provide reference for the application of lncRNAs in crop production and breeding.

Key words: lncRNAs, plant, development, stress response

李建建, 贺宸靖, 黄小平, 向太和. 植物长链非编码RNA调控发育与胁迫应答的研究进展[J]. 生物技术通报, 2023, 39(1): 48-58.

LI Jian-jian, HE Chen-jing, HUANG Xiao-ping, XIANG Tai-he. Research Progress in the Regulation of Development and Stress Response by Long Non-coding RNAs in Plants[J]. Biotechnology Bulletin, 2023, 39(1): 48-58.

图/表 2

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植物Plant	长链非编码RNAs（lncRNAs）	生物学功能Biological functions
拟南芥Arabidopsis thaliana	MAS^[3]、COLDAIR^[5]、COOLAIR^[23]、COLDWRAP^[24]、ASL^[25]、npc48^[26]、FLORE^[27]	开花Flowering
	ENOD40^[11]	侧根发育Lateral root development
	HID1^[20]	光形态发生Photomorphogenesis
	npc536^[26]	盐胁迫Salt stress
	asDOG1^[42]	种子萌发Seed germination
	IPS1^[8]、cis-NATPHO1；2^[44]	磷胁迫Phosphorus stress
	T5120^[48]	氮素胁迫Nitrogen stress
	HAL6^[51]、asHSFB2^[52]、SVALKA^[53]	温度胁迫Temperature stress
	lincRNA13853^[54]、At5NC056820^[55]、DRIR^[56]	干旱胁迫Drought stress
	AtR8^[58-59]	低氧和水杨酸胁迫low oxygen and SA stress
	TE-lincRNA11195^[61]	脱落酸应答Abscisic acid response
	APOLO^[62]	生长素应答Auxin response
	TAR-197/212^[70]、ELENA1^[71]、LINC-AP2^[72]	病原菌应答Pathogen response
水稻Oryza sativa	ENOD40^[13]	根发育Root development
	TL^[21]	叶形态发育Leaf morphological development
	LDMAR^[6]、PMS1T^[29]	光敏育性Photoperiod-sensitive male sterility
	XLOC_057324^[30]	开花Flowering
	EF-cd^[38]	籽粒成熟Grain maturation
	LAIR^[39]	水稻产量Rice yield
	MISSEN^[43]	胚乳发育Endosperm development
	ALEX1^[69]	病原菌应答Pathogen response
番茄Solanum lycopersicum	lncRNA1459^[36]、lncRNA1840^[37]	果实成熟Fruit ripening
	lncRNA16397^[63]、lncRNA23468^[64]、lncRNA33732^[65]、lncRNA39026^[66]、S-slylnc0957^[67]、SILNR1^[68]	病原菌应答Pathogen response
棉花Gossypium hirsutum	Ghir_A01G011040^[17]	纤维发育Fiber development
	XLOC_409583^[18]	株高Plant height
	LncRNA973^[47]	盐胁迫Salt stress
	GhlncNAT-ANX2、GhlncNAT-RLP7^[73]	疾病防御Disease resistance
杨树Populus L.	lnc12、lncWOX11^[10]	不定根发育Adventitious root development
白菜Brassica rapa	BcMF11^[31-32]、BrLMaP^[33]、Bra-eTM160-1/2^[34]	花粉发育Pollen development
大豆Glycine max	ENOD40^[12]	根发育Root development
小麦Triticum aestivum	VAS^[28]	开花Flowering
蒺藜苜蓿Medicago truncatula	ENOD40^[14-15]	根发育Root development
	Mt4^[45-46]	盐胁迫Salt stress
苹果Malus domestica	MSTRG.85814^[57]	营养胁迫Nutrition stress
玉米Zea mays	Zm401^[35]	花粉发育Pollen development
	GARR2^[60]	激素应答Hormone response
密瓜Cucumis melo	LNC-003610^[40]	果实成熟Fruit ripening
苎麻Boehmeria nivea	LTCONS_00034183^[16]	纤维发育Fiber development
柽柳Tamarix hispida	THSAIR6^[49]、THSAIR1-5^[50]	盐胁迫Salt stress

植物Plant	长链非编码RNAs（lncRNAs）	生物学功能Biological functions
拟南芥Arabidopsis thaliana	MAS^[3]、COLDAIR^[5]、COOLAIR^[23]、COLDWRAP^[24]、ASL^[25]、npc48^[26]、FLORE^[27]	开花Flowering
	ENOD40^[11]	侧根发育Lateral root development
	HID1^[20]	光形态发生Photomorphogenesis
	npc536^[26]	盐胁迫Salt stress
	asDOG1^[42]	种子萌发Seed germination
	IPS1^[8]、cis-NATPHO1；2^[44]	磷胁迫Phosphorus stress
	T5120^[48]	氮素胁迫Nitrogen stress
	HAL6^[51]、asHSFB2^[52]、SVALKA^[53]	温度胁迫Temperature stress
	lincRNA13853^[54]、At5NC056820^[55]、DRIR^[56]	干旱胁迫Drought stress
	AtR8^[58-59]	低氧和水杨酸胁迫low oxygen and SA stress
	TE-lincRNA11195^[61]	脱落酸应答Abscisic acid response
	APOLO^[62]	生长素应答Auxin response
	TAR-197/212^[70]、ELENA1^[71]、LINC-AP2^[72]	病原菌应答Pathogen response
水稻Oryza sativa	ENOD40^[13]	根发育Root development
	TL^[21]	叶形态发育Leaf morphological development
	LDMAR^[6]、PMS1T^[29]	光敏育性Photoperiod-sensitive male sterility
	XLOC_057324^[30]	开花Flowering
	EF-cd^[38]	籽粒成熟Grain maturation
	LAIR^[39]	水稻产量Rice yield
	MISSEN^[43]	胚乳发育Endosperm development
	ALEX1^[69]	病原菌应答Pathogen response
番茄Solanum lycopersicum	lncRNA1459^[36]、lncRNA1840^[37]	果实成熟Fruit ripening
	lncRNA16397^[63]、lncRNA23468^[64]、lncRNA33732^[65]、lncRNA39026^[66]、S-slylnc0957^[67]、SILNR1^[68]	病原菌应答Pathogen response
棉花Gossypium hirsutum	Ghir_A01G011040^[17]	纤维发育Fiber development
	XLOC_409583^[18]	株高Plant height
	LncRNA973^[47]	盐胁迫Salt stress
	GhlncNAT-ANX2、GhlncNAT-RLP7^[73]	疾病防御Disease resistance
杨树Populus L.	lnc12、lncWOX11^[10]	不定根发育Adventitious root development
白菜Brassica rapa	BcMF11^[31-32]、BrLMaP^[33]、Bra-eTM160-1/2^[34]	花粉发育Pollen development
大豆Glycine max	ENOD40^[12]	根发育Root development
小麦Triticum aestivum	VAS^[28]	开花Flowering
蒺藜苜蓿Medicago truncatula	ENOD40^[14-15]	根发育Root development
	Mt4^[45-46]	盐胁迫Salt stress
苹果Malus domestica	MSTRG.85814^[57]	营养胁迫Nutrition stress
玉米Zea mays	Zm401^[35]	花粉发育Pollen development
	GARR2^[60]	激素应答Hormone response
密瓜Cucumis melo	LNC-003610^[40]	果实成熟Fruit ripening
苎麻Boehmeria nivea	LTCONS_00034183^[16]	纤维发育Fiber development
柽柳Tamarix hispida	THSAIR6^[49]、THSAIR1-5^[50]	盐胁迫Salt stress